<abstract xmlns="http://www.w3.org/1999/xhtml"><bold>Background</bold>: <italic>Campylobacter</italic> infections are typically self-limited, but in cases with severe enteritis, immuno-compromised system and bacteremia, an appropriate antimicrobial treatment is demanding. Our study aim was to determine the isolation rate of <italic>Campylobacter</italic> among patients with acute enteritis in the capital of North Macedonia and its antimicrobial susceptibility.<bold>Material and methods</bold>: A total number of 3820 patients clinically diagnosed as acute enteritis, were included in the study. Stool samples were collected and <italic>Campylobacter</italic> was isolated and identified by classical microbiological methods. Antimicrobial susceptibility of all isolates to Ceftriaxone, Amoxicillin-clavulonic acid, Erythromycin, Ciprofloxacin, Tetracycline and Gentamicin was determined by disc-diffusion technique. Additionally, minimal inhibitory concentrations of all <italic>Campylobacter</italic> isolates against erythromycin, ciprofloxacin and tetracycline were determined by Epsilon gradient tests.<bold>Results</bold>: <italic>Campylobacter</italic> species was isolated in 97 patients. Although the mean isolation rate of <italic>Campylobacter spp.</italic> during the whole study period was 2.53%, a statistically significant increase was detected in 2016 and 2017, in comparison with the data from previous four years of the study. The isolation rate of <italic>Campylobacter spp.</italic> didn’t reveal statistically significant difference between males and females (p > 0.05). 46.4 % of patients with <italic>Campylobacter</italic> enteritis were children at the age under 15 years. Forty-three <italic>C. jejuni</italic> isolates were susceptible to all six antibiotics, but the remaining 44 isolates revealed resistance to at least one antibiotic. <italic>C. coli</italic> isolates were resistant to 3 antibiotics simultaneously. Two <italic>C. coli</italic> isolates only, were susceptible to all 6 antibiotics. 40.90% of C. jejuni and 50% of <italic>C. coli</italic> isolates were resistant to beta-lactams, fluoroquinolones and tetracyclines, simultaneously.<bold>Conclusion</bold>: The increase of the isolation rate of <italic>Campylobacter</italic> from patients with acute enteritis indicates the need for permanent isolation and identification of <italic>Campylobacter</italic> from every clinically diagnosed patient, as acute enteritis. Erythromicin is the most effective antibiotic for treatment of <italic>Campylobacter</italic> enteritis in our patients. The high level of <italic>Campylobacter</italic> resistance to beta-lactams, fluoroquinolones and tetracyclines requires more rational approach in the treatment of <italic>Campylobacter</italic> enteritis</abstract>

Background: Campylobacter infections are typically self-limited, but in cases with severe enteritis, immuno-compromised system and bacteremia, an appropriate antimicrobial treatment is demanding. Our study aim was to determine the isolation rate of Campylobacter among patients with acute enteritis in the capital of North Macedonia and its antimicrobial susceptibility.Material and methods: A total number of 3820 patients clinically diagnosed as acute enteritis, were included in the study. Stool samples were collected and Campylobacter was isolated and identified by classical microbiological methods. Antimicrobial susceptibility of all isolates to Ceftriaxone, Amoxicillin-clavulonic acid, Erythromycin, Ciprofloxacin, Tetracycline and Gentamicin was determined by disc-diffusion technique. Additionally, minimal inhibitory concentrations of all Campylobacter isolates against erythromycin, ciprofloxacin and tetracycline were determined by Epsilon gradient tests.Results: Campylobacter species was isolated in 97 patients. Although the mean isolation rate of Campylobacter spp. during the whole study period was 2.53%, a statistically significant increase was detected in 2016 and 2017, in comparison with the data from previous four years of the study. The isolation rate of Campylobacter spp. didn’t reveal statistically significant difference between males and females (p > 0.05). 46.4 % of patients with Campylobacter enteritis were children at the age under 15 years. Forty-three C. jejuni isolates were susceptible to all six antibiotics, but the remaining 44 isolates revealed resistance to at least one antibiotic. C. coli isolates were resistant to 3 antibiotics simultaneously. Two C. coli isolates only, were susceptible to all 6 antibiotics. 40.90% of C. jejuni and 50% of C. coli isolates were resistant to beta-lactams, fluoroquinolones and tetracyclines, simultaneously.Conclusion: The increase of the isolation rate of Campylobacter from patients with acute enteritis indicates the need for permanent isolation and identification of Campylobacter from every clinically diagnosed patient, as acute enteritis. Erythromicin is the most effective antibiotic for treatment of Campylobacter enteritis in our patients. The high level of Campylobacter resistance to beta-lactams, fluoroquinolones and tetracyclines requires more rational approach in the treatment of Campylobacter enteritis

Antimicrobial Susceptibility of <italic>Campylobacter</italic> isolates in the Capital of North Macedonia

Institute of Microbiology and Parasitology, Faculty of Medicine

University Clinic of Infectious Diseases and Febrile Conditions

PRILOZI

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 License.

Antimicrobial Susceptibility of  isolates in the Capital of North Macedonia

Background: Campylobacter infections are typically self-limited, but in cases with severe enteritis, immuno-compromised system and bacteremia, an appropriate...

Antimicrobial Susceptibility of Campylobacter isolates in the Capital of North Macedonia

Online veröffentlicht: 11. Okt. 2019

Seitenbereich: 73 - 80

DOI: https://doi.org/10.2478/prilozi-2019-0017

Schlüsselwörter
acute enteritis, antimicrobial susceptibility

© 2019 Elena Trajkovska-Dokic et al., published by Sciendo

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 License.

Antimicrobial Susceptibility of Campylobacter isolates in the Capital of North Macedonia

Online veröffentlicht: 11. Okt. 2019

Seitenbereich: 73 - 80

DOI: https://doi.org/10.2478/prilozi-2019-0017

Schlüsselwörteracute enteritis, antimicrobial susceptibility

© 2019 Elena Trajkovska-Dokic et al., published by Sciendo

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 License.

Schlüsselwörter
acute enteritis, antimicrobial susceptibility