<abstract xml:lang="en" xmlns="http://www.w3.org/1999/xhtml"><p>We extracted <italic>Fusarium solani</italic> antigens to evaluate specific anti-<italic>F. solani</italic> IgE in fifty-one patients with asthma (33 men and 18 women) and in 22 non-atopic healthy subjects (15 men and 7 women). <italic>F. solani</italic> strains were cultured in Sabouraud glucose agar and subjected to cell disruption using the freeze-and-thaw method. The obtained cytoplasmic extracts were analysed using sodium dodecyl sulphate-polyacrylamide gel electrophoresis (SDS-PAGE). Sensitisation to <italic>F. solani</italic> antigens has been evaluated in asthmatic patients using the immunoblotting assay. The SDS-PAGE identified 29 protein bands in the cytoplasmic extracts of <italic>F. solani</italic> isolates, with molecular weights ranging from 24 kDa to 112 kDa. Immunoblotting detected specific anti-<italic>F. solani</italic> IgE antibody in all asthma patients, but not in the control group. The predominant reactive allergens in patients corresponded to the bands with molecular weights of 24 kDa, 58.5 kDa, 64.5 kDa, 69 kDa, 72 kDa, and 97 kDa. Our results suggest that various allergenic components of <italic>F. solani</italic> may produce symptoms of asthma in susceptible individuals and they call for further research.</p></abstract>

We extracted Fusarium solani antigens to evaluate specific anti-F. solani IgE in fifty-one patients with asthma (33 men and 18 women) and in 22 non-atopic healthy subjects (15 men and 7 women). F. solani strains were cultured in Sabouraud glucose agar and subjected to cell disruption using the freeze-and-thaw method. The obtained cytoplasmic extracts were analysed using sodium dodecyl sulphate-polyacrylamide gel electrophoresis (SDS-PAGE). Sensitisation to F. solani antigens has been evaluated in asthmatic patients using the immunoblotting assay. The SDS-PAGE identified 29 protein bands in the cytoplasmic extracts of F. solani isolates, with molecular weights ranging from 24 kDa to 112 kDa. Immunoblotting detected specific anti-F. solani IgE antibody in all asthma patients, but not in the control group. The predominant reactive allergens in patients corresponded to the bands with molecular weights of 24 kDa, 58.5 kDa, 64.5 kDa, 69 kDa, 72 kDa, and 97 kDa. Our results suggest that various allergenic components of F. solani may produce symptoms of asthma in susceptible individuals and they call for further research.

Allergens from <italic>Fusarium solani</italic> Identified by Immunoblotting in Asthma Patients In Iran

Mycology Research Center, Faculty of Veterinary Medicine, University of Tehran, Tehran

Faculty of Veterinary Medicine, University of Mazandaran, Amol

Department of Mycology, Faculty of Medicine, University of Tarbiat Modares, Tehran, Iran

Archives of Industrial Hygiene and Toxicology

Allergens from  Identified by Immunoblotting in Asthma Patients In Iran

We extracted Fusarium solani antigens to evaluate specific anti-F. solani IgE in fifty-one patients with asthma (33 men and 18 women) and in 22 non-atopic...

Allergens from Fusarium solani Identified by Immunoblotting in Asthma Patients In Iran

Online veröffentlicht: 26. März 2012

Seitenbereich: 1 - 6

DOI: https://doi.org/10.2478/10004-1254-63-2012-2155

Schlüsselwörter
alergija na plijesni, IgE, SDS-PAGE, senzibilizacija na alergene, spore u zraku

This content is open access.