<abstract xml:lang="en" xmlns="http://www.w3.org/1999/xhtml">The aim of study was to determine the influence of mobile phone frequency radiation on the proliferation, cytoskeleton structure, and mitotic index of V79 cells after 1 h, 2 h, and 3 h of exposure. V79 cells were cultured in standard laboratory conditions and exposed to continuous-wave (CW) RF/MW radiation of 935 MHz, electric field strength of (8.2±0.3) V m-1, and specific absorption rate (SAR) of 0.12 W kg-1. To identify proliferation kinetics, the cells were counted for each hour of exposure 24 h, 48 h, 72 h, and 96 h after respective exposures. Microtubule proteins were determined using specific immunocytochemical methods. Cell smears were analysed under a fluorescent microscope. The study included negative and positive controls. Mitotic index was determined by estimating the number of dividing cells 24 h after exposure and dividing it with the total number of cells. In comparison to the controls, cell proliferation declined in cells exposed for three hours 72 h after irradiation (p&lt;0.05). Microtubule structure was clearly altered immediately after three hours of irradiation (p&lt;0.05). The mitotic index in RF/MW-exposed cells did not differ from negative controls. However, even if exposure did not affect the number of dividing cells, it may have slowed down cell division kinetics as a consequence of microtubule impairment immediately after exposure.</abstract>

The aim of study was to determine the influence of mobile phone frequency radiation on the proliferation, cytoskeleton structure, and mitotic index of V79 cells after 1 h, 2 h, and 3 h of exposure. V79 cells were cultured in standard laboratory conditions and exposed to continuous-wave (CW) RF/MW radiation of 935 MHz, electric field strength of (8.2±0.3) V m-1, and specific absorption rate (SAR) of 0.12 W kg-1. To identify proliferation kinetics, the cells were counted for each hour of exposure 24 h, 48 h, 72 h, and 96 h after respective exposures. Microtubule proteins were determined using specific immunocytochemical methods. Cell smears were analysed under a fluorescent microscope. The study included negative and positive controls. Mitotic index was determined by estimating the number of dividing cells 24 h after exposure and dividing it with the total number of cells. In comparison to the controls, cell proliferation declined in cells exposed for three hours 72 h after irradiation (p<0.05). Microtubule structure was clearly altered immediately after three hours of irradiation (p<0.05). The mitotic index in RF/MW-exposed cells did not differ from negative controls. However, even if exposure did not affect the number of dividing cells, it may have slowed down cell division kinetics as a consequence of microtubule impairment immediately after exposure.

Disturbance of Cell Proliferation in Response to Mobile Phone Frequency Radiation

Institute for Medical Research and Occupational Health, Zagreb, Croatia

Archives of Industrial Hygiene and Toxicology

The aim of study was to determine the influence of mobile phone frequency radiation on the proliferation, cytoskeleton structure, and mitotic index of V79...

Disturbance of Cell Proliferation in Response to Mobile Phone Frequency Radiation

Online veröffentlicht: 27. März 2009

Seitenbereich: 109 - 115

DOI: https://doi.org/10.2478/10004-1254-60-2009-1891

Schlüsselwörter
brzina stanične proliferacije, mitotski indeks, RF/MW 935 MHz, struktura mikrotubula, trajna kultura stanica

This content is open access.

Disturbance of Cell Proliferation in Response to Mobile Phone Frequency Radiation

Online veröffentlicht: 27. März 2009

Seitenbereich: 109 - 115

DOI: https://doi.org/10.2478/10004-1254-60-2009-1891

Schlüsselwörterbrzina stanične proliferacije, mitotski indeks, RF/MW 935 MHz, struktura mikrotubula, trajna kultura stanica

This content is open access.

Schlüsselwörter
brzina stanične proliferacije, mitotski indeks, RF/MW 935 MHz, struktura mikrotubula, trajna kultura stanica