<abstract xmlns="http://www.w3.org/1999/xhtml"><p>Chronic lymphocytic leukaemia (CLL) manifests as clonal expansion of mature B lymphocytes, whose accumulation is primarily attributed to the dysregulation of apoptosis. Aberrant expression, as well as genetic alterations within various Bcl2 family members and central regulators of the intrinsic, mitochondria-mediated apoptotic pathway all hasve been observed in CLL. Here, we report the expression analysis of the anti-apoptotic Bcl2 gene in a cohort of 58 CLL patients. Quantitative reverse-transcriptase polymerase chain reaction (qRT-PCR) analysis revealed a significant overexpression of Bcl2 mRNA in CLL samples compared to control samples (p=&lt;0.001). Receiver operating characteristic (ROC) analysis showed that the level of Bcl2 expression exerts a high discriminatory power between patients and healthy subjects (A=0.98, 95% CI=0.95-1.009, p&lt;0.0001).</p></abstract>

Chronic lymphocytic leukaemia (CLL) manifests as clonal expansion of mature B lymphocytes, whose accumulation is primarily attributed to the dysregulation of apoptosis. Aberrant expression, as well as genetic alterations within various Bcl2 family members and central regulators of the intrinsic, mitochondria-mediated apoptotic pathway all hasve been observed in CLL. Here, we report the expression analysis of the anti-apoptotic Bcl2 gene in a cohort of 58 CLL patients. Quantitative reverse-transcriptase polymerase chain reaction (qRT-PCR) analysis revealed a significant overexpression of Bcl2 mRNA in CLL samples compared to control samples (p=<0.001). Receiver operating characteristic (ROC) analysis showed that the level of Bcl2 expression exerts a high discriminatory power between patients and healthy subjects (A=0.98, 95% CI=0.95-1.009, p<0.0001).

Expression Of The <italic>Bcl2</italic> Gene In Chronic Lymphocytic Leukaemia Patients

Department of Pharmacy, Faculty of Medical Sciences, University of Kragujevac, Kragujevac, Serbia

Department of Physiology, Faculty of Medical Sciences, University of Kragujevac, Kragujevac, Serbia

Institute of Molecular Genetics and Genetic Engineering, University of Belgrade, Belgrade, Serbia

Clinic for Haematology, Clinical Centre of Serbia, Belgrade, Serbia

Medical Faculty, University of Belgrade, Belgrade, Serbia

Experimental and Applied Biomedical Research (EABR)

This chapter is distributed under the terms of the Creative Commons Attribution 4.0 Public License.

Expression Of The  Gene In Chronic Lymphocytic Leukaemia Patients

Chronic lymphocytic leukaemia (CLL) manifests as clonal expansion of mature B lymphocytes, whose accumulation is primarily attributed to the dysregulation of...

Expression Of The Bcl2 Gene In Chronic Lymphocytic Leukaemia Patients

Online veröffentlicht: 14. Okt. 2015

Seitenbereich: 187 - 191

Eingereicht: 25. Dez. 2014

Akzeptiert: 20. Feb. 2015

DOI: https://doi.org/10.1515/sjecr-2015-0024

Schlüsselwörter
Chronic lymphocytic leukaemia, apoptosis, Bcl2, expression analysis

© 2015 Ksenija Vucicevic et al., published by De Gruyter Open

This chapter is distributed under the terms of the Creative Commons Attribution 4.0 Public License.

Expression Of The Bcl2 Gene In Chronic Lymphocytic Leukaemia Patients

Online veröffentlicht: 14. Okt. 2015

Seitenbereich: 187 - 191

Eingereicht: 25. Dez. 2014

Akzeptiert: 20. Feb. 2015

DOI: https://doi.org/10.1515/sjecr-2015-0024

SchlüsselwörterChronic lymphocytic leukaemia, apoptosis, Bcl2, expression analysis

© 2015 Ksenija Vucicevic et al., published by De Gruyter Open

This chapter is distributed under the terms of the Creative Commons Attribution 4.0 Public License.

Schlüsselwörter
Chronic lymphocytic leukaemia, apoptosis, Bcl2, expression analysis