<abstract xmlns="http://www.w3.org/1999/xhtml">

<p>As the test material mink feed with natural microflora was used. The analyses were conducted using Wrzosek and TPGY broth media, and Willis–Hobbs and Zeissler differential agar media. Wrzosek, Willis–Hobbs, and Zeissler media are described in Polish Standards approved by the National Standards Body in Poland and routinely used in detection of anaerobic bacteria in Poland. Detection and identification of <italic>C. botulinum</italic> was performed with a previously validated real-time PCR method based on <italic>ntnh</italic> gene detection, which is common in all <italic>C. botulinum</italic> toxotypes. The use of Wrzosek broth and Zeissler agar in routine analyses for detection and identification of <italic>C. botulinum</italic> was ineffective and limited. The obtained results showed the highest culturing process effectiveness in TPGY broth with 72 h incubation at 30°C and isolation on Willis–Hobbs agar. The real-time PCR method based on <italic>ntnh</italic> gene detection used in this study could be utilised as a supplementary tool to the mouse lethality assay.</p>
</abstract>

As the test material mink feed with natural microflora was used. The analyses were conducted using Wrzosek and TPGY broth media, and Willis–Hobbs and Zeissler differential agar media. Wrzosek, Willis–Hobbs, and Zeissler media are described in Polish Standards approved by the National Standards Body in Poland and routinely used in detection of anaerobic bacteria in Poland. Detection and identification of C. botulinum was performed with a previously validated real-time PCR method based on ntnh gene detection, which is common in all C. botulinum toxotypes. The use of Wrzosek broth and Zeissler agar in routine analyses for detection and identification of C. botulinum was ineffective and limited. The obtained results showed the highest culturing process effectiveness in TPGY broth with 72 h incubation at 30°C and isolation on Willis–Hobbs agar. The real-time PCR method based on ntnh gene detection used in this study could be utilised as a supplementary tool to the mouse lethality assay.

Optimisation of protocol for <italic>Clostridium botulinum</italic> detection in mink feed

Department of Hygiene of Animal Feedingstuffs

Journal of Veterinary Research

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Optimisation of protocol for  detection in mink feed

As the test material mink feed with natural microflora was used. The analyses were conducted using Wrzosek and TPGY broth media, and Willis–Hobbs and Zeissler...

Optimisation of protocol for Clostridium botulinum detection in mink feed

Online veröffentlicht: 30. Sept. 2015

Seitenbereich: 377 - 382

Eingereicht: 26. März 2015

Akzeptiert: 03. Sept. 2015

DOI: https://doi.org/10.1515/bvip-2015-0056

Schlüsselwörter
mink, feed, culture techniques, real-time PCR

© 2015 National Veterinary Research Institute in Pulawy

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Optimisation of protocol for Clostridium botulinum detection in mink feed

Online veröffentlicht: 30. Sept. 2015

Seitenbereich: 377 - 382

Eingereicht: 26. März 2015

Akzeptiert: 03. Sept. 2015

DOI: https://doi.org/10.1515/bvip-2015-0056

Schlüsselwörtermink, feed, culture techniques, real-time PCR

© 2015 National Veterinary Research Institute in Pulawy

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Schlüsselwörter
mink, feed, culture techniques, real-time PCR