<abstract xmlns="http://www.w3.org/1999/xhtml"><p>The study of cell proliferation is a useful tool in the fields of toxicology, pathophysiology and pharmacology. Cell proliferation and its degree can be evaluated using 5-bromo-2′-deoxyuridine which is incorporated into the newly synthesized DNA. The aim of this study was the optimization of subcutaneous application of 5-bromo-2′-deoxyuridine implantation for continuous and persistent marking of proliferating cells in the rat forestomach. 3-<italic>tert</italic>-Butyl-4-hydroxyanisole was used as the agent that ensures cell proliferation. In order to determine the optimal dose for proliferating cells labeling, 5-bromo-2′-deoxyuridine doses of 50 mg, 100 mg, 200 mg or 350 mg were implemented 2 days prior to sacrifice by flat-faced cylindrical matrices. Immunohistochemical analysis using 5-bromo-2′-deoxyuridine <italic>in situ</italic> detection kit was performed for the detection of 5-bromo-2′-deoxyuridine labeled cells. The results showed that for adult rats, the optimum 5-bromo-2′-deoxyuridine dose is 200 mg per animal for subcutaneous application. The here described manner of 5-bromo-2′-deoxyuridine <italic>in vivo</italic> labeling provides a simple, efficient, and reliable method for cell labeling, and at the same minimizes stress to animals.</p></abstract>

The study of cell proliferation is a useful tool in the fields of toxicology, pathophysiology and pharmacology. Cell proliferation and its degree can be evaluated using 5-bromo-2′-deoxyuridine which is incorporated into the newly synthesized DNA. The aim of this study was the optimization of subcutaneous application of 5-bromo-2′-deoxyuridine implantation for continuous and persistent marking of proliferating cells in the rat forestomach. 3-tert-Butyl-4-hydroxyanisole was used as the agent that ensures cell proliferation. In order to determine the optimal dose for proliferating cells labeling, 5-bromo-2′-deoxyuridine doses of 50 mg, 100 mg, 200 mg or 350 mg were implemented 2 days prior to sacrifice by flat-faced cylindrical matrices. Immunohistochemical analysis using 5-bromo-2′-deoxyuridine in situ detection kit was performed for the detection of 5-bromo-2′-deoxyuridine labeled cells. The results showed that for adult rats, the optimum 5-bromo-2′-deoxyuridine dose is 200 mg per animal for subcutaneous application. The here described manner of 5-bromo-2′-deoxyuridine in vivo labeling provides a simple, efficient, and reliable method for cell labeling, and at the same minimizes stress to animals.

Cell proliferation assay – method optimisation for <italic>in vivo</italic> labeling of DNA in the rat forestomach

Vinča Institute of Nuclear Sciences, University of Belgrade, Mike Petrovica Alasa 12-14, 11001 Belgrade,

Institute for Medical Research, University of Belgrade, Dr. Subotića Street 4, 11129 Belgrade,

Faculty of Pharmacy, University of Belgrade, Vojvode Stepe 450, 11221 Belgrade,

Acta Veterinaria

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Cell proliferation assay – method optimisation for  labeling of DNA in the rat forestomach

The study of cell proliferation is a useful tool in the fields of toxicology, pathophysiology and pharmacology. Cell proliferation and its degree can be...

Cell proliferation assay – method optimisation for in vivo labeling of DNA in the rat forestomach

Online veröffentlicht: 30. März 2017

Seitenbereich: 1 - 10

Eingereicht: 30. Juni 2016

Akzeptiert: 18. Okt. 2016

DOI: https://doi.org/10.1515/acve-2017-0001

Schlüsselwörter
5-bromo-2′-deoxyuridine, 3--Butyl-4-hydroxyanisole, BrdU labeling, cell proliferation, rat forestomach

© 2017 Joksić Gordana et al., published by De Gruyter Open

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Cell proliferation assay – method optimisation for in vivo labeling of DNA in the rat forestomach

Online veröffentlicht: 30. März 2017

Seitenbereich: 1 - 10

Eingereicht: 30. Juni 2016

Akzeptiert: 18. Okt. 2016

DOI: https://doi.org/10.1515/acve-2017-0001

Schlüsselwörter5-bromo-2′-deoxyuridine, 3--Butyl-4-hydroxyanisole, BrdU labeling, cell proliferation, rat forestomach

© 2017 Joksić Gordana et al., published by De Gruyter Open

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Schlüsselwörter
5-bromo-2′-deoxyuridine, 3--Butyl-4-hydroxyanisole, BrdU labeling, cell proliferation, rat forestomach