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Zeitschriften
Asian Biomedicine
Band 12 (2018): Heft 1 (February 2018)
Uneingeschränkter Zugang
Bioactivity of a sol–gel-derived hydroxyapatite coating on titanium implants in vitro and in vivo
Jintamai Suwanprateeb
Jintamai Suwanprateeb
,
Waraporn Suvannapruk
Waraporn Suvannapruk
,
Watchara Chokevivat
Watchara Chokevivat
,
Siripong Kiertkrittikhoon
Siripong Kiertkrittikhoon
,
Nara Jaruwangsanti
Nara Jaruwangsanti
und
Prakit Tienboon
Prakit Tienboon
| 31. Dez. 2018
Asian Biomedicine
Band 12 (2018): Heft 1 (February 2018)
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Article Category:
Brief communication (original)
Online veröffentlicht:
31. Dez. 2018
Seitenbereich:
35 - 44
DOI:
https://doi.org/10.1515/abm-2018-0029
Schlüsselwörter
bone–implant interface
,
bone remodeling
,
hydroxyapatites
,
osseointegration
,
sol–gel process
© 2018 Jintamai Suwanprateeb et al., published by Sciendo
This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 License.
Figure 1
Comparison of cell proliferation on the surfaces between 20 uncoated and 20 coated (gray bars) titanium samples by methyl thiazolyl tetrazolium (MTT) assay (error bars, standard deviation, n = 4 per type per time point). Absorbance by the MTT formazan chromophore at 570 nm, and D indicates the time of incubation in days. *P < 0.05
Figure 2
Comparison of cell mineralization on the surfaces of 20 uncoated and 20 coated (gray bars) titanium samples by Alizarin red S assay (error bars, standard deviation, n = 4 per type per time point). Absorbance by the Alizarin red S at 570 nm, and D indicates the time of incubation in days. *P < 0.05
Figure 3
Scanning electron microscopy (SEM) images of osteoblast morphology on uncoated and coated titanium samples. a) uncoated 1 day; b) coated 1 day; c) uncoated 3 days; d) coated 3 days; e) uncoated 7 days; f ) coated 7 days; g) uncoated 14 days; h) coated 14 days; i) uncoated 21 days; and j) coated 21 days (magnification ×1000). Scale bars represent 60 μm
Figure 4
Extraction torque from bone of 12 uncoated and 12 coated titanium screws at different implantation periods (wks, weeks) (error bars, standard deviation, n = 4 per type per time point). *P < 0.05
Figure 5
Scanning electron microscopy (SEM) image of uncoated and coated screws extracted from the bone. No detachment of the coating from the underlying screw is noted. Arrows indicate bone fragments (BFs). The amount and size of the BFs increased with increasing implantation periods. a) uncoated 7 weeks; b) coated 7 weeks; c) uncoated 12 weeks; d) coated 12 weeks; e) uncoated 24 weeks; and f ) coated 24 weeks (magnification ×100). Scale bars represent 1 mm
Figure 6
Histology of bone healing around the hydroxyapatite-coated and -uncoated screws in New Zealand white rabbits at 7 weeks, 12 weeks, and 24 weeks after surgery. Hematoxylin and eosin staining demonstrates bone remodeling comprising bone trabeculae with mixed woven bone and lamellar bone. Mature lamellar bone is only observed in coated sample at 24 weeks of implantation. a) uncoated 7 weeks; b) coated 7 weeks; c) uncoated 12 weeks; d) coated 12 weeks; e) uncoated 24 weeks; and f ) coated 24 weeks (magnification ×100; Ti, titanium; B, bone). Scale bars represent 200 μm
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