<abstract xmlns="http://www.w3.org/1999/xhtml">The real-time monitoring of alcoholic fermentation (sugar consumption) is very important in industrial processes. Several techniques (i.e., using a biosensor) have been proposed to realize this goal. In this work, we propose a new method to follow sugar yeast consumption. This novel method is based on the changes in the medium resistance (Rm) that are induced by the CO2 bubbles produced during a fermentative process. We applied a 50-mV and 700-Hz signal to 75 ml of a yeast suspension in a tripolar cell. A gold electrode was used as the working electrode, whereas an Ag/AgCl electrode and a stainless-steel electrode served as the reference and counter electrodes, respectively. We then added glucose to the yeast suspension and obtained a 700% increase in the Rm after 8 minutes. The addition of sucrose instead of glucose as the carbon source resulted in a 1200% increase in the Rm. To confirm that these changes are the result of CO2 bubbles in the fermentation medium, we designed a tetrapolar cell in which CO2 gas was insufflated at the bottom of the cell and concluded that the changes were due to CO2 bubbles produced during the fermentation. Consequently, this new method is a low-cost and rapid technology to follow the sugar consumption in yeast.</abstract>

The real-time monitoring of alcoholic fermentation (sugar consumption) is very important in industrial processes. Several techniques (i.e., using a biosensor) have been proposed to realize this goal. In this work, we propose a new method to follow sugar yeast consumption. This novel method is based on the changes in the medium resistance (Rm) that are induced by the CO2 bubbles produced during a fermentative process. We applied a 50-mV and 700-Hz signal to 75 ml of a yeast suspension in a tripolar cell. A gold electrode was used as the working electrode, whereas an Ag/AgCl electrode and a stainless-steel electrode served as the reference and counter electrodes, respectively. We then added glucose to the yeast suspension and obtained a 700% increase in the Rm after 8 minutes. The addition of sucrose instead of glucose as the carbon source resulted in a 1200% increase in the Rm. To confirm that these changes are the result of CO2 bubbles in the fermentation medium, we designed a tetrapolar cell in which CO2 gas was insufflated at the bottom of the cell and concluded that the changes were due to CO2 bubbles produced during the fermentation. Consequently, this new method is a low-cost and rapid technology to follow the sugar consumption in yeast.

Evaluation of sugar yeast consumption by measuring electrical medium resistance

Journal of Electrical Bioimpedance

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

{"article-title":"Evaluation of sugar yeast consumption by measuring electrical medium resistance"}

The real-time monitoring of alcoholic fermentation (sugar consumption) is very important in industrial processes. Several techniques (i.e., using a biosensor)...

Evaluation of sugar yeast consumption by measuring electrical medium resistance

Article Category: Articles

Published Online: Dec 10, 2013

Page range: 51 - 56

Received: Jul 29, 2013

DOI: https://doi.org/10.5617/jeb.664

Keywords
Yeast, sugar consumption, monitoring, CO, medium resistance

© 2013 Martín L. Zamora, Gabriel A. Ruiz, Carmelo J. Felice, published by Sciendo

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

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Evaluation of sugar yeast consumption by measuring electrical medium resistance

Article Category: Articles

Published Online: Dec 10, 2013

Page range: 51 - 56

Received: Jul 29, 2013

DOI: https://doi.org/10.5617/jeb.664

KeywordsYeast, sugar consumption, monitoring, CO, medium resistance

© 2013 Martín L. Zamora, Gabriel A. Ruiz, Carmelo J. Felice, published by Sciendo

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Fig. 1

Fig. 2

Fig. 3

Fig. 4

Fig. 5

Fig. 6

Fig. 7

Fig. 8

Fig. 9

Keywords
Yeast, sugar consumption, monitoring, CO, medium resistance