A folic acid-induced rat model of renal injury to identify biomarkers of tubulointerstitial fibrosis from urinary exosomes
, , , , , and | Mar 31, 2017
About this article
Article Category: Technical report (Original)
Published Online: Mar 31, 2017
Page range: 491 - 502
DOI: https://doi.org/10.5372/1905-7415.1005.513
Keywords
© 2016 Panthip Rattanasinganchan, Kittipat Sopitthummakhun, Kent Doi, Xuzhen Hu, D. Michael Payne, Trairak Pisitkun, Asada Leelahavanichkul
This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.
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Annexin family proteins identified in rat urine exosomes
| RefSeq number | Protein name | Normalized spectral counts | ||
|---|---|---|---|---|
| Vehicle | 1 week post-FA | 2 weeks post-FA | ||
| Group 1: found in relatively moderate abundance in all 3 samples | ||||
| NP_001011918.1 | Annexin A11 | 56 | 91 | 111 |
| NP_077069.3 | Annexin A4 | 50 | 41 | 9 |
| NP_063970.1 | Annexin A2 | 18 | 74 | 29 |
| Group 2: found in low abundance and only in some samples | ||||
| XP_001071546.1 | PREDICTED: similar to Annexin A10 | 9 | 6 | 0 |
| NP_037036.1 | Annexin A1 | 6 | 6 | 0 |
| XP_001068572.1 | PREDICTED: similar to annexin A13 | 6 | 0 | 3 |
| NP_569100.1 | Annexin A7 | 2 | 13 | 2 |
| Group 3: found in moderate abundance only in exosome samples from FA-treated rats | ||||
| NP_037264.1 | Annexin A5 | 0 | 69 | 47 |
Comparison of the most abundant proteins (estimated from normalized spectral counts) identified in urine exosomal samples from vehicle-treated rats and rats 1 and 2 weeks after folic acid (FA) treatment.
| Ref. Sequence number | Protein name | Normalized spectral counts | ||
|---|---|---|---|---|
| Vehicle | 1 week post-FA | 2 weeks post-FA | ||
| NP_954887.1 | Cystatin-related protein 2 precursor | 602 | 216 | 403 |
| NP_061998.1 | Probasin precursor | 373 | 243 | 446 |
| NP_446292.1 | Gamma-glutamyltransferase 1 Membrane protein abundantly/predominantly expressed in the kidney | 282 | 308 | 192 |
| NP_598306.1 | Common salivary protein 1 | 195 | 132 | 333 |
| NP_037025.1 | Actin, gamma-enteric smooth muscle | 191 | 226 | 314 |
| NP_037347.1 | Aflatoxin B1 aldehyde reductase member 3 | 187 | 3 | 24 |
| NP036740.1 | Neprilysin Membrane protein abundantly/predominantly expressed in the kidney | 181 | 101 | 269 |
| NP_001002807.1 | Chloride intracellular channel protein 1 | 178 | 62 | 75 |
| NP_113902.1 | Ribonuclease UK114 | 175 | 51 | 66 |
| NP_036767.1 | Anionic trypsin-1 precursor | 175 | 362 | 443 |
| NP_058778.1 | Uromodulin precursor Membrane protein abundantly/predominantly expressed in the kidney | 160 | 70 | 318 |
| NP_112274.1 | Aminopeptidase N precursor Membrane protein abundantly/predominantly expressed in the kidney | 153 | 109 | 218 |
| NP_058912.1 | Neutral and basic amino acid transport protein rBAT Membrane protein abundantly/predominantly expressed in the kidney | 152 | 47 | 132 |
| NP_037315.1 | Meprin A subunit beta precursor | 115 | 17 | 80 |
| NP_997476.1 | Prostatic steroid-binding protein C2 | 112 | 20 | 531 |
| NP_036947.1 | Glutamate–cysteine ligase catalytic subunit | 105 | 0 | 17 |
| NP_001005889.2 | Radixin | 101 | 39 | 84 |
| NP_543180.1 | ADP-ribosylation factor 3 | 99 | 50 | 39 |
| NP_036850.1 | Cystatin-related protein 1 precursor | 97 | 97 | 608 |
| NP_059001.1 | Glutamate–cysteine ligase regulatory subunit | 95 | 7 | 18 |
| NP_071964.2 | Alpha-1-antiproteinase precursor Absent in control exosome sample, and present in both 1-week (inflammation) and 2-week (fibrosis) exosome samples from FA-treated rats | 0 | 75 | 178 |
| NP_037264.1 | Annexin A5 Absent in control exosome sample, and present in both 1-week (inflammation) and 2-week (fibrosis) exosome samples from FA-treated rats | 0 | 69 | 47 |
| XP_001055138.1 | PREDICTED: similar to putative breast adenocarcinoma marker | 0 | 32 | 0 |
| NP_444180.2 | Clusterin precursor Absent in control exosome sample, and present in both 1-week (inflammation) and 2-week (fibrosis) exosome samples from FA-treated rats | 0 | 31 | 22 |
| XP_001055585.1 | PREDICTED: similar to grancalcin | 0 | 23 | 0 |
| NP_01025081.1 | Programmed cell death 6 interacting protein (ALIX) | 0 | 21 | 10 |
| NP_476487.1 | Complement component C9 precursor | 0 | 16 | 0 |
| NP_068611.1 | Histone H2A type 4 | 0 | 15 | 8 |
| NP_599153.1 | Albumin | 0 | 15 | 13 |
| NP_620796.1 | Galectin-3-binding protein precursor | 0 | 14 | 7 |
| NP_058821.1 | CD63 antigen | 0 | 13 | 8 |
| NP_059055.2 | Ras-related protein Rab-10 Absent in control exosome sample, and present in both 1-week (inflammation) and 2-week (fibrosis) exosome samples from FA-treated rats | 0 | 10 | 25 |
| NP_001019475.1 | Glutathione S-transferase Mu 4 | 0 | 9 | 9 |
| NP_803435.1 | Prostatic steroid-binding protein C1 precursor Absent in control exosome sample, and present in both 1-week (inflammation) and 2-week (fibrosis) exosome samples from FA-treated rats | 0 | 9 | 18 |
| NP_001004236.1 | Tetraspanin-1 | 0 | 8 | 4 |
| NP_071605.2 | Complement decay-accelerating factor precursor | 0 | 8 | 5 |
| NP_073204.1 | Transglutaminase 4 (prostate) | 0 | 7 | 6 |
| NP_446145.1 | Cyclin-D-binding Myb-like transcription factor 1 | 0 | 7 | 0 |
| NP_036638.1 | Sodium/potassium-transporting ATPase subunit alpha-3 | 0 | 7 | 3 |
| NP_01013087.1 | PCTP-like protein | 0 | 7 | 0 |
| NP_071964.2 | Alpha-1-antiproteinase precursor Absent in control exosome sample, and present in both 1-week (inflammation) and 2-week (fibrosis) exosome samples from FA-treated rats | 0 | 75 | 178 |
| NP_037264.1 | Annexin A5 Absent in control exosome sample, and present in both 1-week (inflammation) and 2-week (fibrosis) exosome samples from FA-treated rats | 0 | 69 | 47 |
| XP_001070423.1 | PREDICTED: hypothetical protein | 0 | 0 | 45 |
| NP_788265.1 | S100 calcium-binding protein, ventral prostate | 0 | 0 | 33 |
| XP_01055098.1 | PREDICTED: similar to carbonyl reductase 3 | 0 | 4 | 29 |
| XP_001068139.1 | PREDICTED: hypothetical protein | 0 | 0 | 27 |
| NP_067599.1 | Myoglobin | 0 | 0 | 26 |
| NP_059055.2 | Ras-related protein Rab-10 Absent in control exosome sample, and present in both 1-week (inflammation) and 2-week (fibrosis) exosome samples from FA-treated rats | 0 | 10 | 25 |
| NP_445937.1 | Protein S100-A6 | 0 | 0 | 22 |
| NP_444180.2 | Clusterin precursor Absent in control exosome sample, and present in both 1-week (inflammation) and 2-week (fibrosis) exosome samples from FA-treated rats | 0 | 31 | 22 |
| NP_037245.1 | ATPase, Na+/K+ transporting, beta 1 polypeptide | 0 | 7 | 20 |
| NP_803435.1 | Prostatic steroid-binding protein C1 precursor Absent in control exosome sample, and present in both 1-week (inflammation) and 2-week (fibrosis) exosome samples from FA-treated rats | 0 | 9 | 18 |
| NP_001020832.1 | Protein crumbs homolog 3 precursor | 0 | 0 | 18 |
| NP_112313.1 | Macrophage migration inhibitory factor | 0 | 0 | 17 |
| XP_01060853.1 | PREDICTED: similar to ATPase, H+ transporting, lysosomal | |||
| Accessory protein 2 | 0 | 0 | 16 | |
| NP_036792.2 | Elongation factor 1-alpha 2 | 0 | 2 | 15 |
| NP_001013169.1 | Guanine nucleotide-binding protein subunit alpha-14 | 0 | 0 | 14 |
| NP_446316.1 | Transitional endoplasmic reticulum ATPase | 0 | 2 | 14 |
| NP_071636.1 | Brain acid soluble protein 1 | 0 | 0 | 14 |
| NP_068512.1 | Monocyte differentiation antigen CD14 precursor | 0 | 0 | 13 |