<abstract xmlns="http://www.w3.org/1999/xhtml"><sec id="j_1905-7415.1004.502_s_001_w2aab3b7c10b1b6b1aab1c16b1Aa"><h3>Background</h3><p>Acute infection of pregnant women with <italic>Toxoplasma gondii</italic> can result in fetal transmission. When clinical abnormalities are evident by ultrasonography in late gestation, medical treatment may be attempted, but the data on outcomes are limited.</p></sec><sec id="j_1905-7415.1004.502_s_002_w2aab3b7c10b1b6b1aab1c16b2Aa"><h3>Objectives</h3><p>To present a case of congenital fetal toxoplasmosis from Thailand and review the literature.</p></sec><sec id="j_1905-7415.1004.502_s_003_w2aab3b7c10b1b6b1aab1c16b3Aa"><h3>Methods</h3><p>A woman presented at 31 weeks of gestation with severe bilateral ventriculomegaly, cardiomegaly, hepatosplenomegaly with intrahepatic hyperechogenicities, and polyhydramnios. Medical treatment with pyrimethamine, sulfadiazine and folinic acid was initiated. There was no improvement. Social, medical, ethical and legal issues were considered.</p></sec><sec id="j_1905-7415.1004.502_s_004_w2aab3b7c10b1b6b1aab1c16b4Aa"><h3>Results</h3><p>Delivery was indicated at 34<sup>+2</sup> weeks of gestation because of premature amniorrhexis. The neonate suffered from respiratory distress, meningitis, ventilator-associated pneumonia, and expired at age 11 days.</p></sec><sec id="j_1905-7415.1004.502_s_005_w2aab3b7c10b1b6b1aab1c16b5Aa"><h3>Conclusions</h3><p>When fetal congenital toxoplasmosis is diagnosed late in pregnancy with severe ultrasonographic abnormalities, the neonatal outcome is poor despite in utero treatment with antibiotics.</p></sec></abstract>

BackgroundAcute infection of pregnant women with Toxoplasma gondii can result in fetal transmission. When clinical abnormalities are evident by ultrasonography in late gestation, medical treatment may be attempted, but the data on outcomes are limited.ObjectivesTo present a case of congenital fetal toxoplasmosis from Thailand and review the literature.MethodsA woman presented at 31 weeks of gestation with severe bilateral ventriculomegaly, cardiomegaly, hepatosplenomegaly with intrahepatic hyperechogenicities, and polyhydramnios. Medical treatment with pyrimethamine, sulfadiazine and folinic acid was initiated. There was no improvement. Social, medical, ethical and legal issues were considered.ResultsDelivery was indicated at 34+2 weeks of gestation because of premature amniorrhexis. The neonate suffered from respiratory distress, meningitis, ventilator-associated pneumonia, and expired at age 11 days.ConclusionsWhen fetal congenital toxoplasmosis is diagnosed late in pregnancy with severe ultrasonographic abnormalities, the neonatal outcome is poor despite in utero treatment with antibiotics.


<div xmlns="http://www.w3.org/1999/xhtml"><p>Toxoplasmosis is caused by the parasite <italic>Toxoplasma gondii</italic>. Acute infection in pregnant women can result in vertical transmission to the fetus, causing multiple sequelae that can present during the prenatal and neonatal period [<a ref-type="bibr" href="#j_1905-7415.1004.502_ref_001_w2aab3b7c10b1b6b1ab1b8b1Aa">1</a>]. In France and Germany, routine screening is performed in pregnant women to detect seroconversion for <italic>T. gondii</italic> antibodies [<a ref-type="bibr" href="#j_1905-7415.1004.502_ref_002_w2aab3b7c10b1b6b1ab1b8b2Aa">2</a>]. In such circumstances, fetal infection is usually detected and treatment administered before ultrasonographic abnormalities develop. The majority of fetuses (98.4%) are then delivered without clinical symptoms of infection [<a ref-type="bibr" href="#j_1905-7415.1004.502_ref_003_w2aab3b7c10b1b6b1ab1b8b3Aa">3</a>].</p><p>Mothers are not universally screened in other countries [<a ref-type="bibr" href="#j_1905-7415.1004.502_ref_002_w2aab3b7c10b1b6b1ab1b8b2Aa">2</a>, <a ref-type="bibr" href="#j_1905-7415.1004.502_ref_004_w2aab3b7c10b1b6b1ab1b8b4Aa">4</a>]. Infected fetuses are then often diagnosed once clinical abnormalities are detected on routine ultrasonography. Therapeutic termination of pregnancy may be offered if the diagnosis is made before fetal viability. However, when the fetus is diagnosed during late gestation, counseling becomes challenging, as prognosis is poor. Yet data on outcomes following in utero treatment of clinically-affected fetuses remains limited. We report a case of severe congenital toxoplasmosis diagnosed in late pregnancy.</p><p>The report of this case was approved by the Human Research Ethics Committee, Faculty of Medicine Prince Songkla University (Rec No. 57-282-12-1). The patient provided written informed consent for this publication.</p><sec id="j_1905-7415.1004.502_s_006_w2aab3b7c10b1b6b1ab1b3Aa"><div>Case report</div><p>A 41-year-old Thai gravida 3, parity 2, at 31<sup>+1</sup> weeks of gestation, was referred for evaluation of abnormal cerebral ultrasound findings in her fetus. Her previous pregnancy history, antenatal history, and ultrasonographic findings at 17 weeks of gestation for amniocentesis were unremarkable. The fetal chromosomal study was normal. She did not own a cat, but had been feeding a stray cat near her home. She had never been screened for <italic>T. gondii</italic> antibodies.</p><p>Ultrasonography at 31<sup>+1</sup> weeks of gestation revealed severe bilateral ventriculomegaly with areas of hyperechogenicities in the periventricular region (<bold><a ref-type="fig" href="#j_1905-7415.1004.502_fig_001_w2aab3b7c10b1b6b1ab1b3b3Aa">Figure 1</a></bold>), cardiomegaly, hepatosplenomegaly with intrahepatic hyperechogenicites (<bold><a ref-type="fig" href="#j_1905-7415.1004.502_fig_002_w2aab3b7c10b1b6b1ab1b3b4Aa">Figure 2</a></bold>), and polyhydramnios. Fetal congenital infection was suspected and fetal blood sampling was performed and sent for serologic testing. The result was positive for <italic>T. gondii</italic> IgG and IgM antibodies.</p><figure id="j_1905-7415.1004.502_fig_001_w2aab3b7c10b1b6b1ab1b3b3Aa" position="float" fig-type="figure"><h2>Figure 1</h2><figCaption><p>Ultrasound image. Transventricular view of the fetal head shows severe dilatation of lateral ventricles with thinning of the cerebral cortex and periventricular calcification. The lateral ventricle measures 35.9 mm.</p></figCaption><img xmlns:xlink="http://www.w3.org/1999/xlink" xlink:href="graphic/j_1905-7415.1004.502_fig_001.jpg" src="https://sciendo-parsed.s3.eu-central-1.amazonaws.com/6470666183f1392090d68bca/j_1905-7415.1004.502_fig_001.jpg?X-Amz-Algorithm=AWS4-HMAC-SHA256&amp;X-Amz-Date=20240519T213324Z&amp;X-Amz-SignedHeaders=host&amp;X-Amz-Expires=18000&amp;X-Amz-Credential=AKIA6AP2G7AKP25APDM2%2F20240519%2Feu-central-1%2Fs3%2Faws4_request&amp;X-Amz-Signature=deca6283c2c5e687569688bb71584def2b33383838e08f2f7d3c7a642e6786dc" class="mw-100"/></figure><figure id="j_1905-7415.1004.502_fig_002_w2aab3b7c10b1b6b1ab1b3b4Aa" position="float" fig-type="figure"><h2>Figure 2</h2><figCaption><p>Ultrasound image. Cross-sectional view of the fetal abdomen shows liver enlargement with diffuse intrahepatic calcifications</p></figCaption><img xmlns:xlink="http://www.w3.org/1999/xlink" xlink:href="graphic/j_1905-7415.1004.502_fig_002.jpg" src="https://sciendo-parsed.s3.eu-central-1.amazonaws.com/6470666183f1392090d68bca/j_1905-7415.1004.502_fig_002.jpg?X-Amz-Algorithm=AWS4-HMAC-SHA256&amp;X-Amz-Date=20240519T213324Z&amp;X-Amz-SignedHeaders=host&amp;X-Amz-Expires=18000&amp;X-Amz-Credential=AKIA6AP2G7AKP25APDM2%2F20240519%2Feu-central-1%2Fs3%2Faws4_request&amp;X-Amz-Signature=216086a895f285a26ca72732a332b416ce523a99fa837ca8ff6627c04e3ff610" class="mw-100"/></figure><p>After counseling, the patient was started with written informed consent on pyrimethamine, sulfadiazine and folinic acid (PSF regimen) at 32<sup>+1</sup> weeks of gestation. No adverse effects related to the medications were observed during the treatment period. Ultrasonography was repeated at 34<sup>+2</sup> weeks of gestation and showed similar findings. On the same day, she developed premature amniorrhexis and went into labor.</p><p>The fetus was delivered by cesarean section because of previous section. The female neonate weighed 1,920 g, which was appropriate for her gestational age. The Apgar scores at 1 and 5 minutes were 7 and 8, respectively. She was intubated because of respiratory distress, which later was diagnosed as congenital pneumonia and respiratory distress syndrome. An initial examination revealed a normal head circumference of 30 cm, jaundice, and hepatosplenomegaly. An eye examination showed iris atrophy and vitreous haziness compatible with toxoplasmosis. The IgG test was positive in serum and cerebrospinal fluid. The placental showed chronic villitis with calcifications and encysted organisms in trophoblastic cells (<bold><a ref-type="fig" href="#j_1905-7415.1004.502_fig_003_w2aab3b7c10b1b6b1ab1b3b7Aa">Figure 3</a></bold>), findings compatible with toxoplasmosis.</p><figure id="j_1905-7415.1004.502_fig_003_w2aab3b7c10b1b6b1ab1b3b7Aa" position="float" fig-type="figure"><h2>Figure 3</h2><figCaption><p>Pathological image. Encysted organisms in the trophoblastic cells of the placenta (red arrow), hematoxylin and eosin stain. The size of the parasites can be estimated from the erythrocytes (approximately 7 μm diameter).</p></figCaption><img xmlns:xlink="http://www.w3.org/1999/xlink" xlink:href="graphic/j_1905-7415.1004.502_fig_003.jpg" src="https://sciendo-parsed.s3.eu-central-1.amazonaws.com/6470666183f1392090d68bca/j_1905-7415.1004.502_fig_003.jpg?X-Amz-Algorithm=AWS4-HMAC-SHA256&amp;X-Amz-Date=20240519T213324Z&amp;X-Amz-SignedHeaders=host&amp;X-Amz-Expires=18000&amp;X-Amz-Credential=AKIA6AP2G7AKP25APDM2%2F20240519%2Feu-central-1%2Fs3%2Faws4_request&amp;X-Amz-Signature=d71ab4655890ed65a0dca19b26a31bb3c9f8de711e234622f778e2adbab6d201" class="mw-100"/></figure><p>The PSF regimen was given to the newborn. Despite this treatment, her clinical course was complicated with neonatal sepsis, meningitis and ventilator-associated pneumonia. Death occurred at the age of 11 days.</p></sec><sec id="j_1905-7415.1004.502_s_007_w2aab3b7c10b1b6b1ab1b4Aa"><div>Discussion</div><p>We report a case of congenital toxoplasmosis diagnosed at late gestation when the fetus demonstrated multiple abnormalities on ultrasonography including ventriculomegaly, periventricular calcifications, cardiomegaly, hepatosplenomegaly with intrahepatic hyperechogenicities, and polyhydramnios.</p><p>Humans are usually infected via eating under-cooked infected meat, or ingesting food contaminated with toxoplasma oocysts. The seroprevalence of toxoplasmosis in women of a child-bearing age varies by geographical region, ranging from 0.8% in South Korea to 77% in Brazil [<a ref-type="bibr" href="#j_1905-7415.1004.502_ref_002_w2aab3b7c10b1b6b1ab1b8b2Aa">2</a>]. In Thailand, 19%–28.3% of pregnant women have been found to be seropositive for <italic>T. gondii</italic> antibodies [<a ref-type="bibr" href="#j_1905-7415.1004.502_ref_005_w2aab3b7c10b1b6b1ab1b8b5Aa">5</a>, <a ref-type="bibr" href="#j_1905-7415.1004.502_ref_006_w2aab3b7c10b1b6b1ab1b8b6Aa">6</a>].</p><p>When pregnant women become infected with <italic>T. gondii</italic>, most remain asymptomatic, yet parasites can be transmitted to their fetus across the placenta. Risk depends on timing of infection and is estimated to be 10%-25% in the first trimester and increases to 60%–90% in the third trimester. Conversely, clinical manifestations are more severe the earlier the infection occurs [<a ref-type="bibr" href="#j_1905-7415.1004.502_ref_001_w2aab3b7c10b1b6b1ab1b8b1Aa">1</a>]. Ultrasonographic findings include hydrops, ventricular dilatation, intracranial calcifications, intrahepatic calcifications, pleural effusion, pericardial effusion, and a thick, echogenic placenta. Abnormal intracranial findings indicate severe fetal infection and a poor prognosis [<a ref-type="bibr" href="#j_1905-7415.1004.502_ref_007_w2aab3b7c10b1b6b1ab1b8b7Aa">7</a>]. Fetal infection is usually diagnosed by fetal blood sampling for antibody detection or amniotic fluid polymerase chain reaction (PCR).</p><p>When the fetuses are severely affected, therapeutic termination of pregnancy is usually offered [<a ref-type="bibr" href="#j_1905-7415.1004.502_ref_008_w2aab3b7c10b1b6b1ab1b8b8Aa">8</a>, <a ref-type="bibr" href="#j_1905-7415.1004.502_ref_009_w2aab3b7c10b1b6b1ab1b8b9Aa">9</a>]. According to the International Federation of Gynecology and Obstetrics (FIGO), when termination of pregnancy following prenatal diagnosis is performed beyond 22 weeks of gestation, it must be preceded by feticide to ensure delivery of a stillborn fetus [<a ref-type="bibr" href="#j_1905-7415.1004.502_ref_010_w2aab3b7c10b1b6b1ab1b8c10Aa">10</a>].</p><p>In Thailand, abortion is legal if performed by a physician to protect the health of the mother and in pregnancy that resulted from sexual crimes. Pregnancy termination, when severe fetal anomalies are diagnosed, and in order to preserve the mothers’ mental health is acceptable according to new medical legislation published in the Royal Thai Government Gazette [<a ref-type="bibr" href="#j_1905-7415.1004.502_ref_011_w2aab3b7c10b1b6b1ab1b8c11Aa">11</a>]. However, for nonlethal anomalies, pregnancy termination is possible only before the pregnancy reaches the threshold of viability, usually before 22 to 24 weeks of gestation, depending on the institution. Feticide for fetal anomaly is not acceptable in Thailand and termination of pregnancy at late gestation for nonlethal anomaly is considered inappropriate because the fetus may be born alive and suffer additional complications from prematurity.</p><p>In the present case, the gestational age of the fetus at diagnosis was beyond the threshold of viability and the abnormalities found were nonlethal. These ethical and legal limitations were discussed with the patient and invasive fetal testing was recommended only to confirm the diagnosis and to guide whether further fetal treatment can be offered.</p><p>After the diagnosis of congenital toxoplasmosis was confirmed, the patient and her husband were informed about the results and that the fetus carried the risk of stillbirth and severe neurological deficits if she survived. Despite the poor prognosis, and after discussion with parents, we decided to administer antibiotics in the hope of minimizing morbidity. This management was based on few reported cases of clinically-affected fetuses that were treated. Two cases in 2005 and 2011 involved severely-affected fetuses, who had residual abnormalities in postnatal life despite treatment in utero [<a ref-type="bibr" href="#j_1905-7415.1004.502_ref_012_w2aab3b7c10b1b6b1ab1b8c12Aa">12</a>, <a ref-type="bibr" href="#j_1905-7415.1004.502_ref_013_w2aab3b7c10b1b6b1ab1b8c13Aa">13</a>]. However, Friedman, reported a case in 1999, of fetal hydrops caused by congenital toxoplasmosis, in which the fetus showed improvement after the initiation of the PSF regimen, and the neonate had only minimal abnormalities in postnatal life [<a ref-type="bibr" href="#j_1905-7415.1004.502_ref_014_w2aab3b7c10b1b6b1ab1b8c14Aa">14</a>]. A large retrospective analysis from Germany in 2010, also indicated effectiveness of prenatal treatment of toxoplasmosis, even in fetuses with abnormal prenatal findings [<a ref-type="bibr" href="#j_1905-7415.1004.502_ref_003_w2aab3b7c10b1b6b1ab1b8b3Aa">3</a>]. In that study, there were 3 cases of prenatally-diagnosed fetal ventriculomegaly; all of them experienced disease improvement, as evidenced via either subsequent prenatal ultrasonography or other postnatal imaging. The authors postulated that if the treatment was initiated as early as possible (not later than 12 weeks following maternal infection) it could be beneficial. The details of each case are summarized in <bold><a ref-type="table" href="#j_1905-7415.1004.502_tab_001_w2aab3b7c10b1b6b1ab1b4b8Aa">Table 1</a></bold>.</p><table-wrap id="j_1905-7415.1004.502_tab_001_w2aab3b7c10b1b6b1ab1b4b8Aa" position="float"><label>Table 1</label><caption><p>Clinical summary of patients with prenatal findings of congenital toxoplasmosis described in the literature and our patient</p></caption><table rules="groups"><thead><tr><th align="left">Author (year)</th><th align="left">Gestational age (GA) at diagnosis</th><th align="left">Diagnostic methods</th><th align="left">GA at treatment</th><th align="left">Age at delivery</th><th align="left">Treatment</th><th align="left">Prenatal findings</th><th align="left">Postnatal outcomes</th></tr></thead><tbody><tr><td align="left">Friedman et al. 1999 [<a ref-type="bibr" href="#j_1905-7415.1004.502_ref_014_w2aab3b7c10b1b6b1ab1b8c14Aa">14</a>]</td><td align="left">27 weeks</td><td align="left">IgM, polymerase chain reaction (PCR)</td><td align="left">28 weeks</td><td align="left">39 weeks</td><td align="left">Pyrimethamine, sulfadiazine, and folinic acid (PSF)</td><td align="left">Ventriculomegaly Ascites Pleural effusion Pericardial effusion</td><td align="left">Chorioretinitis Periventricular calcification Normal development at 4 months</td></tr><tr><td align="left">Nowokowska et al. 2005 [<a ref-type="bibr" href="#j_1905-7415.1004.502_ref_012_w2aab3b7c10b1b6b1ab1b8c12Aa">12</a>]20052005</td><td align="left">27 weeks</td><td align="left">IgG, IgM, IgG avidity, PCR</td><td align="left">28 weeks</td><td align="left">38 weeks</td><td align="left">Spiramycin then PSF Shunt placement at 35 wks</td><td align="left">Hydrocephalus Clubfoot</td><td align="left">Hydrocephalus Intracranial calcifications Seizure Chorioretinitis Alive</td></tr><tr><td align="left">Tamaru et al. 2011 [<a ref-type="bibr" href="#j_1905-7415.1004.502_ref_013_w2aab3b7c10b1b6b1ab1b8c13Aa">13</a>]012011</td><td align="left">23 weeks</td><td align="left">IgG, IgM</td><td align="left">25 weeks</td><td align="left">32 weeks</td><td align="left">Sulfadoxine, pyrimethamine, azithromycin, acetylspiramycin</td><td align="left">Ventriculomegaly Ascites Pericardial effusion Cardiomegaly Placentomegaly</td><td align="left">Ventriculomegaly Ascites Hepatosplenomegaly Chorioretinitis Meningitis Alive at 7 months</td></tr><tr><td align="left">Present case</td><td align="left">31 weeks</td><td align="left">IgG, IgM</td><td align="left">32 weeks</td><td align="left">34 weeks</td><td align="left">PSF</td><td align="left">Ventriculomegaly Cardiomegaly Hepatosplenomegaly Hydramnios</td><td align="left">Ventriculomegaly Hepatosplenomegaly Iris atrophy Meningitis Neonatal death</td></tr></tbody></table></table-wrap><p>For the present case, the duration of maternal infection could not be determined because the mother was asymptomatic and no <italic>T. gondii</italic> screening was conducted before presentation. The patient received only 2 weeks of medications before delivery. The poor perinatal outcome resulted not only from the toxoplasmosis infection, but also from the preterm delivery and superimposed complications of prematurity, such as respiratory distress syndrome. However, multiple clinical manifestations of congenital toxoplasmosis were demonstrated in the newborn and in the placental histology, indicating poor response to prenatal medical therapy. We conclude that late detection of the infection, severe clinical manifestations in the fetus, late treatment, and the short duration of treatment-to-delivery time contributed to the poor outcome.</p></sec><sec id="j_1905-7415.1004.502_s_008_w2aab3b7c10b1b6b1ab1b5Aa"><div>Conclusion</div><p>From our experience, when fetal congenital toxoplasmosis is diagnosed late in pregnancy with severe ultrasonographic abnormalities, the neonatal outcome is likely to be poor despite in utero treatment with antibiotics. Premature delivery and short treatment duration may worsen the prognosis for the fetus.</p></sec></div>

Prenatal diagnosis and in utero treatment of severe congenital toxoplasmosis: a case report

Asian Biomedicine

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

BackgroundAcute infection of pregnant women with Toxoplasma gondii can result in fetal transmission. When clinical abnormalities are evident by ultrasonography...

Prenatal diagnosis and in utero treatment of severe congenital toxoplasmosis: a case report

Article Category: Clinical report

Published Online: Jan 31, 2017

Page range: 387 - 391

DOI: https://doi.org/10.5372/1905-7415.1004.502

Keywords
Congenital infection, congenital toxoplasmosis, prenatal diagnosis, social and ethical considerations, therapy of fetus, ultrasonography

© 2016 Chusana Petpichetchian, Thitima Suntharasaj, Ounjai Kor-Anantakul

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Figure 1

Figure 2

Figure 3

Clinical summary of patients with prenatal findings of congenital toxoplasmosis described in the literature and our patient

Author (year)	Gestational age (GA) at diagnosis	Diagnostic methods	GA at treatment	Age at delivery	Treatment	Prenatal findings	Postnatal outcomes
Friedman et al. 1999 [14]	27 weeks	IgM, polymerase chain reaction (PCR)	28 weeks	39 weeks	Pyrimethamine, sulfadiazine, and folinic acid (PSF)	Ventriculomegaly Ascites Pleural effusion Pericardial effusion	Chorioretinitis Periventricular calcification Normal development at 4 months
Nowokowska et al. 2005 [12]20052005	27 weeks	IgG, IgM, IgG avidity, PCR	28 weeks	38 weeks	Spiramycin then PSF Shunt placement at 35 wks	Hydrocephalus Clubfoot	Hydrocephalus Intracranial calcifications Seizure Chorioretinitis Alive
Tamaru et al. 2011 [13]012011	23 weeks	IgG, IgM	25 weeks	32 weeks	Sulfadoxine, pyrimethamine, azithromycin, acetylspiramycin	Ventriculomegaly Ascites Pericardial effusion Cardiomegaly Placentomegaly	Ventriculomegaly Ascites Hepatosplenomegaly Chorioretinitis Meningitis Alive at 7 months
Present case	31 weeks	IgG, IgM	32 weeks	34 weeks	PSF	Ventriculomegaly Cardiomegaly Hepatosplenomegaly Hydramnios	Ventriculomegaly Hepatosplenomegaly Iris atrophy Meningitis Neonatal death

Prenatal diagnosis and in utero treatment of severe congenital toxoplasmosis: a case report

Article Category: Clinical report

Published Online: Jan 31, 2017

Page range: 387 - 391

DOI: https://doi.org/10.5372/1905-7415.1004.502

KeywordsCongenital infection, congenital toxoplasmosis, prenatal diagnosis, social and ethical considerations, therapy of fetus, ultrasonography

© 2016 Chusana Petpichetchian, Thitima Suntharasaj, Ounjai Kor-Anantakul

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Figure 1

Figure 2

Figure 3

Clinical summary of patients with prenatal findings of congenital toxoplasmosis described in the literature and our patient

Keywords
Congenital infection, congenital toxoplasmosis, prenatal diagnosis, social and ethical considerations, therapy of fetus, ultrasonography