<abstract xmlns="http://www.w3.org/1999/xhtml"><sec id="j_1905-7415.0904.423_s_001_w2aab3b7c10b1b6b1aab1c16b1Aa"><h3>Background</h3><p>Current data on the prevalence of hemoglobinopathies among Lahu in northern Thailand are limited.</p></sec><sec id="j_1905-7415.0904.423_s_002_w2aab3b7c10b1b6b1aab1c16b2Aa"><h3>Objective</h3><p>To investigate the prevalence of thalassemia carriers among Lahu hill tribe people in Chiang Rai Province, Thailand.</p></sec><sec id="j_1905-7415.0904.423_s_003_w2aab3b7c10b1b6b1aab1c16b3Aa"><h3>Materials and Methods</h3><p>We conducted a cross-sectional study in 2 phases. The first retrospectively analyzed data from antenatal clinics attended by Lahu women between January 2011 and June 2012. The second phase was prospective and included the husbands of Lahu women. In the second phase, 116 Lahu adults were administered a questionnaire and blood tests for osmotic fragility (OFT) and dichlorophenol indophenol precipitation (DCIP). The hemoglobin (Hb) type of those positive for either or both tests was identified by HPLC and PCR.</p></sec><sec id="j_1905-7415.0904.423_s_004_w2aab3b7c10b1b6b1aab1c16b4Aa"><h3>Results</h3><p>Data from 358 Lahu women in the first phase showed a mean age of 23.2 y (range 13-46 y, SD 6.83), 68.5% were primigravida. Fifty-eight had abnormal mean corpuscular volume (MCV), 87 positive OFT, 18 positive DCIP test, and 3.0% positive results in both tests. Eight of 83 participating husbands had abnormal MCV, 8 positive OFT and 2 positive DCIP test. In the second phase, 52.2% of 116 participants were women, mean age of 33.7 y (range 18-68 y, SD 11.2), 38 positive OFT, 10 positive DCIP test, and 3.7% positive results in both tests. Hb typing showed 5 participants with HbE, 1 β-thalassemic, 1 HbE homozygous, and no α-thalassemia-1 (SEA) was identified.</p></sec><sec id="j_1905-7415.0904.423_s_005_w2aab3b7c10b1b6b1aab1c16b5Aa"><h3>Conclusion</h3><p>Provision of a thalassemia screening in health care settings in remote areas of Thailand is an ongoing need.</p></sec></abstract>

BackgroundCurrent data on the prevalence of hemoglobinopathies among Lahu in northern Thailand are limited.ObjectiveTo investigate the prevalence of thalassemia carriers among Lahu hill tribe people in Chiang Rai Province, Thailand.Materials and MethodsWe conducted a cross-sectional study in 2 phases. The first retrospectively analyzed data from antenatal clinics attended by Lahu women between January 2011 and June 2012. The second phase was prospective and included the husbands of Lahu women. In the second phase, 116 Lahu adults were administered a questionnaire and blood tests for osmotic fragility (OFT) and dichlorophenol indophenol precipitation (DCIP). The hemoglobin (Hb) type of those positive for either or both tests was identified by HPLC and PCR.ResultsData from 358 Lahu women in the first phase showed a mean age of 23.2 y (range 13-46 y, SD 6.83), 68.5% were primigravida. Fifty-eight had abnormal mean corpuscular volume (MCV), 87 positive OFT, 18 positive DCIP test, and 3.0% positive results in both tests. Eight of 83 participating husbands had abnormal MCV, 8 positive OFT and 2 positive DCIP test. In the second phase, 52.2% of 116 participants were women, mean age of 33.7 y (range 18-68 y, SD 11.2), 38 positive OFT, 10 positive DCIP test, and 3.7% positive results in both tests. Hb typing showed 5 participants with HbE, 1 β-thalassemic, 1 HbE homozygous, and no α-thalassemia-1 (SEA) was identified.ConclusionProvision of a thalassemia screening in health care settings in remote areas of Thailand is an ongoing need.

Prevalence of thalassemia carriers among the Lahu hill tribe population, Chiang Rai, Thailand

Asian Biomedicine

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

BackgroundCurrent data on the prevalence of hemoglobinopathies among Lahu in northern Thailand are limited.ObjectiveTo investigate the prevalence of...

Charcteristic	n	%
Total	368	100.00
Age (years)
≤20	149	40.5
21–30	161	43.8
31–40	50	13.6
≥40	8	2.1
min = 13 y, max = 46 y, mean = 23.23 y, SD = 6.83 y
Gravidity
1	162	68.5
≥2	75	31.5
Mean corpuscular volume
Abnormal (<80 fL)	58	17.6
Normal (80–100 fL)	271	82.4
Hematocrit
Abnormal (<33%)	94	29.1
Normal (≥33%)	230	70.9
OFT
Positive	87	23.6
Negative	274	74.5
Unknown		1.9
DCIP test
Positive	18	5.0
Negative	342	95.0

Charcateristic	n	%
Total	83	100
Age (years)
≤2 0	7	19
21–30	20	56
31–40	7	19
≥4 0	2	6
MCV
Abnormal (<80 fL)	8	17
Normal (80-100 fL)	40	83
OFT
Positive	8	10
Negative	75	90
DCIP test
Positive	2	3
Negative	81	97

Charcateristic	n	%
Total	116	100
Osmotic fragility test
Positive	38	33
Negative	78	67
Dichlorophenol indophenol precipitation test
Positive	10	9
Negative	106	91
Typing total	38	100
A2A (Hb A2: 2.0%–3.1%) (normal type)	31	82
A2A (Hb A2: 4.9%) (b-thalassemia trait)	1	3
EA (HbE trait)	5	13
EE (HbE Homozygous)	1	3
α-Thalassemia 1 (SEA)	0	0

Prevalence of thalassemia carriers among the Lahu hill tribe population, Chiang Rai, Thailand

Article Category: Brief communication (Original)

Published Online: Jan 31, 2017

Page range: 527 - 533

DOI: https://doi.org/10.5372/1905-7415.0904.423

Keywords
Hill tribe, Lahu, pregnant women, prevalence, thalassemia carrier

© 2015 Tawatchai Apidechkul

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Osmotic fragility test (OFT) and dichlorophenol indophenol precipitation (DCIP) test results for thalassemia screening of pregnant Lahu women receiving antenatal care between January 2011 and June 2012

Osmotic fragility test (OFT) and dichlorophenol indophenol precipitation (DCIP) test for thalassemia screening of the husbands of pregnant women who were found positive for either or both of OFT and DCIP tests between January 2011 and June 2012

Thalassemia identification by HPLC and PCR techniques

Prevalence of thalassemia carriers among the Lahu hill tribe population, Chiang Rai, Thailand

Article Category: Brief communication (Original)

Published Online: Jan 31, 2017

Page range: 527 - 533

DOI: https://doi.org/10.5372/1905-7415.0904.423

KeywordsHill tribe, Lahu, pregnant women, prevalence, thalassemia carrier

© 2015 Tawatchai Apidechkul

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Osmotic fragility test (OFT) and dichlorophenol indophenol precipitation (DCIP) test results for thalassemia screening of pregnant Lahu women receiving antenatal care between January 2011 and June 2012

Osmotic fragility test (OFT) and dichlorophenol indophenol precipitation (DCIP) test for thalassemia screening of the husbands of pregnant women who were found positive for either or both of OFT and DCIP tests between January 2011 and June 2012

Thalassemia identification by HPLC and PCR techniques

Keywords
Hill tribe, Lahu, pregnant women, prevalence, thalassemia carrier