<abstract xmlns="http://www.w3.org/1999/xhtml"><sec id="j_1905-7415.0903.397_s_001_w2aab3b7b3b1b6b1aab1c16b1Aa"><h3>Background</h3><p>Dermatophytosis is a superficial infection caused by filamentous fungi belonging to the following three genera: Microsporum, Trichophyton and Epidermophyton. Arbitrarily primed PCR (AP-PCR) is a rapid and sensitive procedure for the diagnosis of the fungal species.</p></sec><sec id="j_1905-7415.0903.397_s_002_w2aab3b7b3b1b6b1aab1c16b2Aa"><h3>Objectives</h3><p>To identify various dermatophyte species as rapidly and precisely as possible.</p></sec><sec id="j_1905-7415.0903.397_s_003_w2aab3b7b3b1b6b1aab1c16b3Aa"><h3>Methods</h3><p>Fifty-two clinical dermatophyte isolates from ten species were recovered from samples obtained the Department of Medical Mycology and patients in different parts of Iran. All 52 dermatophyte isolates tested belonged to any of Trichophyton, Microsporum, or Epidermophyton genera. Four random primers, OPAA11, OPU15, OPAA17, and OPD18, were used in this study.</p></sec><sec id="j_1905-7415.0903.397_s_004_w2aab3b7b3b1b6b1aab1c16b4Aa"><h3>Results</h3><p>The results indicated that all 10 dermatophyte species displayed distinct DNA band patterns after amplification with the random primers OPAA11 and OPU15. Nine species of dermatophytes were distinguished with the random primer OPAA17 using a different DNA band pattern. AP-PCR amplified different PCR products using primer OPD18.</p></sec><sec id="j_1905-7415.0903.397_s_005_w2aab3b7b3b1b6b1aab1c16b5Aa"><h3>Conclusions</h3><p>AP-PCR offers a convenient solution to the problems encountered in the recognition of morphological features of dermatophyte species in comparison with conventional morphological methods. This approach represents technological progress in identification of dermatophytosis.</p></sec></abstract>

BackgroundDermatophytosis is a superficial infection caused by filamentous fungi belonging to the following three genera: Microsporum, Trichophyton and Epidermophyton. Arbitrarily primed PCR (AP-PCR) is a rapid and sensitive procedure for the diagnosis of the fungal species.ObjectivesTo identify various dermatophyte species as rapidly and precisely as possible.MethodsFifty-two clinical dermatophyte isolates from ten species were recovered from samples obtained the Department of Medical Mycology and patients in different parts of Iran. All 52 dermatophyte isolates tested belonged to any of Trichophyton, Microsporum, or Epidermophyton genera. Four random primers, OPAA11, OPU15, OPAA17, and OPD18, were used in this study.ResultsThe results indicated that all 10 dermatophyte species displayed distinct DNA band patterns after amplification with the random primers OPAA11 and OPU15. Nine species of dermatophytes were distinguished with the random primer OPAA17 using a different DNA band pattern. AP-PCR amplified different PCR products using primer OPD18.ConclusionsAP-PCR offers a convenient solution to the problems encountered in the recognition of morphological features of dermatophyte species in comparison with conventional morphological methods. This approach represents technological progress in identification of dermatophytosis.

Identification of dermatophytes by arbitrarily primed PCR

Health Research Institute, Infectious and Tropical Diseases Research Center

Jundishapur University of Medical Sciences

Department of Medical Mycology, Medical School

Ahvaz Jundishapur University of Medical Sciences

Asian Biomedicine

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

{"article-title":"Identification of dermatophytes by arbitrarily primed PCR"}

BackgroundDermatophytosis is a superficial infection caused by filamentous fungi belonging to the following three genera: Microsporum, Trichophyton and...

Identification of dermatophytes by arbitrarily primed PCR

Article Category: Original article

Published Online: Jan 31, 2017

Page range: 291 - 298

DOI: https://doi.org/10.5372/1905-7415.0903.397

Keywords
AP-PCR, dermatophytosis, dermatophyte, random primer

© 2015 Majid Zarrin, Zahra Salehi, Ali Zarei Mahmoudabadi

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Identification of dermatophytes by arbitrarily primed PCR

Article Category: Original article

Published Online: Jan 31, 2017

Page range: 291 - 298

DOI: https://doi.org/10.5372/1905-7415.0903.397

KeywordsAP-PCR, dermatophytosis, dermatophyte, random primer

© 2015 Majid Zarrin, Zahra Salehi, Ali Zarei Mahmoudabadi

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Keywords
AP-PCR, dermatophytosis, dermatophyte, random primer