Original article. A novel reverse transcription polymerase chain reaction reveals a high prevalence of Plasmodium vivax gametocyte carriage in an endemic area of Thailand

1. Hay SI, Guerra CA, Tatem AJ, Noor AM, Snow RW. The global distribution and population at risk of malaria: past, present and future. Lancet Infect Dis. 2004; 4:327-36.10.1016/S1473-3099(04)01043-6Open DOISearch in Google Scholar

2. Baird JK. Neglect of Plasmodium vivax malaria. Trends Parasitol. 2007; 23:533-9.10.1016/j.pt.2007.08.01117933585Open DOISearch in Google Scholar

3. Mendis K, Sina BJ, Marchesini P, Carter R. The neglected burden of Plasmodium vivax malaria. Am J Trop Med Hyg. 2001; 64:97-106.10.4269/ajtmh.2001.64.9711425182Search in Google Scholar

4. Aide P, Bassat Q, Alonso PL. Towards an effective malaria vaccine. Arch Dis Child. 2007; 92:476-9.10.1136/adc.2005.092551206617817515617Open DOISearch in Google Scholar

5. Cui L, Yan G, Sattabongkot J, Cao Y, Chen B, Chen X, et al. Malaria in the Greater Mekong subregion: Heterogeneity and complexity. Acta Trop. 2012; 121: 227-39.10.1016/j.actatropica.2011.02.016313257921382335Search in Google Scholar

6. Drakeley C, Sutherland C, Bousema JT, Sauerwein RW, Targett GA. The epidemiology of Plasmodium falciparum gametocytes: weapons of mass dispersion. Trends Parasitol. 2006; 22:424-30.10.1016/j.pt.2006.07.00116846756Open DOISearch in Google Scholar

7. Karl S, Davis TM, St. Pierre TG. A comparison of the sensitivities of detection of Plasmodium falciparum gametocytes by magnetic fractionation, thick blood film microscopy, and RT-PCR. Malar J. 2009; 8:98.10.1186/1475-2875-8-98268925519432971Search in Google Scholar

8. Dowling MA, Shute GT. A comparative study of thick and thin blood films in the diagnosis of scanty malaria parasitemia. Bull World Health Organ. 1966; 34:249-67.Search in Google Scholar

9. Bousema T, Drakeley C. Epidemiology and infectivity of Plasmodium falciparum and Plasmodium vivax gametocytes in relation to malaria control and elimination. Clin Microbiol Rev. 2011; 24:377-410.10.1128/CMR.00051-10312248921482730Open DOISearch in Google Scholar

10. Putaporntip C, Hongsrimuang T, Seethamchai S, Kobasa T, Limkittikul K, Cui L, et al. Differential prevalence of Plasmodium infections and cryptic Plasmodium knowlesi malaria in humans in Thailand. J Infect Dis. 2009; 199:1143-50.10.1086/597414881762319284284Search in Google Scholar

11. Jongwutiwes S, Buppan P, Kosuvin R, Seethamchai S, Pattanawong U, Sirichaisinthop J, et al. Plasmodium knowlesi malaria in humans and macaques, Thailand. Emerg Infect Dis. 2011; 17:1799-806.10.3201/eid1710.110349331067322000348Open DOISearch in Google Scholar

12. Putaporntip C, Buppan P, Jongwutiwes S. Improved performance with saliva and urine as alternative DNA sources for malaria diagnosis by mitochondrial DNA-based PCR assays. Clin Microbiol Infect. 2011; 17:1484-91.10.1111/j.1469-0691.2011.03507.xOpen DOISearch in Google Scholar

13. Baker DA. Malaria gametocytogenesis. Mol Biochem Parasitol. 2010; 172:57-65.10.1016/j.molbiopara.2010.03.019Search in Google Scholar

14. Pradel G. Proteins of the malaria parasite sexual stages: expression, function and potential for transmission blocking strategies. Parasitology. 2007; 134:1911-29.10.1017/S0031182007003381Search in Google Scholar

15. Lasonder E, Ishihama Y, Andersen JS, Vermunt AM, Pain A, Sauerwein RW, et al. Analysis of the Plasmodium falciparum proteome by high-accuracy mass spectrometry. Nature. 2002; 419:537-42.10.1038/nature01111Search in Google Scholar

16. Alano P, Billker O. Gametocytes and gametes. In Molecular approaches to malaria. 1st edition. Edited by Sherman IW. Washington DC: ASM Press; 2005: 191-219.10.1128/9781555817558.ch10Search in Google Scholar

17. Ou☐draogo AL, Bousema T, Schneider P, de Vlas SJ, Ilboudo-Sanogo E, Cuzin-Ouattara N, et al. Substantial contribution of submicroscopical Plasmodium falciparum gametocyte carriage to the infectious reservoir in an area of seasonal transmission. PLoS One. 2009; 4:e8410.10.1371/journal.pone.0008410Open DOISearch in Google Scholar

18. Schneider P, Bousema JT, Gouagna LC, Otieno S, van de Vegte-Bolmer M, Omar SA, et al. Submicroscopic Plasmodium falciparum gametocyte densities frequently result in mosquito infection. Am J Trop Med Hyg. 2007; 76:470-4.10.4269/ajtmh.2007.76.470Search in Google Scholar

19. Babiker HA, Abdel-Wahab A, Ahmed S, Suleiman S, Ranford-Cartwright L, Carter R, et al. Detection of low level Plasmodium falciparum gametocytes using reverse transcriptase polymerase chain reaction. Mol Biochem Parasitol. 1999; 9:143-8.10.1016/S0166-6851(98)00175-3Open DOISearch in Google Scholar

20. Bousema T, Okell L, Shekalaghe S, Griffin JT, Omar S, Sawa P, et al. Revisiting the circulation time of Plasmodium falciparum gametocytes: molecular detection methods to estimate the duration of gametocyte carriage and the effect of gametocytocidal drugs. Malar J. 2010; 9:136.10.1186/1475-2875-9-136288193820497536Search in Google Scholar

21. Malikul S. The current situation of the anti-malaria programme in Thailand. Southeast Asian J Trop Med Pub Health. 1988; 19:355-9.Search in Google Scholar

22. Bharti AR, Chuquiyauri R, Brouwer KC, Stancil J, Lin J, Llanos-Cuentas A, et al. Experimental infection of the neotropical malaria vector Anopheles darlingi by human patient-derived Plasmodium vivax in the Peruvian Amazon. Am J Trop Med Hyg. 2006; 75: 610-6.10.4269/ajtmh.2006.75.610Search in Google Scholar

23. Beurskens M, Mens P, Schallig H, Syafruddin D, Asih PB, Hermsen R, et al. Quantitative determination of Plasmodium vivax gametocytes by real-time quantitative nucleic acid sequence-based amplification in clinical samples. Am J Trop Med Hyg. 2009; 81: 366-9.10.4269/ajtmh.2009.81.366Search in Google Scholar

24. Sattabongkot J, Maneechai N, Rosenberg R. Plasmodium vivax: gametocyte infectivity of naturally infected Thai adults. Parasitology. 1991; 102:27-31.10.1017/S0031182000060303Open DOISearch in Google Scholar

25. Smalley ME, Brown J, Bassett NM. The rate of production of Plasmodium falciparum gametocytes during natural infections. Trans Roy Soc Trop Med Hyg. 1981; 75:318-9.10.1016/0035-9203(81)90349-7Search in Google Scholar

26. Nacher M, Carrara VI, McGready R, Ashley E, Nguen JV, Thwai KL, et al. Seasonal fluctuations in the carriage of Plasmodium vivax gametocytes in Thailand. Ann Trop Med Parasitol. 2004; 98:115-20.10.1179/000349804225003145Open DOISearch in Google Scholar

27. Nakazawa S, Culleton R, Maeno Y. In vivo and in vitro gametocyte production of Plasmodium falciparum isolates from Northern Thailand. Int J Parasitol. 2011; 41:317-23.10.1016/j.ijpara.2010.10.003Open DOISearch in Google Scholar

28. Maeno Y, Nakazawa S, Nagashima S, Sasaki J, Higo KM, Taniguchi K. Utility of the dried blood on filter paper as a source of cytokine mRNA for the analysis of immunoreactions in Plasmodium yoelii infection. Acta Trop. 2003; 87:295-300.10.1016/S0001-706X(03)00096-2Search in Google Scholar

29. Maeno Y, Nakazawa S, Dao le D, Yamamoto N, Giang ND, Van Hanh T, et al. A dried blood sample on filter paper is suitable for detecting Plasmodium falciparum gametocytes by reverse transcription polymerase chain reaction. Acta Trop. 2008; 107:121-7.10.1016/j.actatropica.2008.05.00118554563Search in Google Scholar