<abstract xmlns="http://www.w3.org/1999/xhtml"> Background: Tinospora crispa (TC) has been used for years as diabetic remedy in Thai-traditional medicine. It has recently been reported that the aqueous extract from TC’s dried stem enhances glucose transport of L6 myotubes.Objective: Investigate the effects of the water-ethanol extract of T. crispa (WETC) on glucose transport in a rat muscle cell-line L6 myotubes, and explore the mechanism of action of T. crispa in muscle cells.Materials and methods: Glucose uptake rate was determined with the [3H] 2-deoxyglucose uptake method and the cell viability was assessed by sulforhodamine B (SRB) assay. Expressions of glucose transporter 1 (GLUT1), GLUT4, extracellular signal regulated kinase 1/2 (ERK1/2), and AMP-activated protein kinase (AMPK) were measured by quantitative Reverse transcriptase-polymerase chain reaction (RT-PCR) and Western blot analysis.Results: At 400 μg/mL, WETC significantly stimulated glucose uptake into L6 myotubes after 24 hours incubation (246.1 0.1% above basal; p &lt;0.05). The enhancement of glucose transport by WETC was blocked by cycloheximide, a protein synthesis inhibitor. The up-regulation of GLUT1 protein was accompanied by a significant increased in GLUT1 mRNA expression. ERK1/2 was significantly activated in WETC-treated L6 myotubes. In addition, WETC treatment increased AMPK activity in L6 myotubes.Conclusion: T. crispa increased glucose transport activity of L6 myotubes by enhancing GLUT1 expression, a result of AMPK stimulation. </abstract>

Background: Tinospora crispa (TC) has been used for years as diabetic remedy in Thai-traditional medicine. It has recently been reported that the aqueous extract from TC’s dried stem enhances glucose transport of L6 myotubes.Objective: Investigate the effects of the water-ethanol extract of T. crispa (WETC) on glucose transport in a rat muscle cell-line L6 myotubes, and explore the mechanism of action of T. crispa in muscle cells.Materials and methods: Glucose uptake rate was determined with the [3H] 2-deoxyglucose uptake method and the cell viability was assessed by sulforhodamine B (SRB) assay. Expressions of glucose transporter 1 (GLUT1), GLUT4, extracellular signal regulated kinase 1/2 (ERK1/2), and AMP-activated protein kinase (AMPK) were measured by quantitative Reverse transcriptase-polymerase chain reaction (RT-PCR) and Western blot analysis.Results: At 400 μg/mL, WETC significantly stimulated glucose uptake into L6 myotubes after 24 hours incubation (246.1 0.1% above basal; p <0.05). The enhancement of glucose transport by WETC was blocked by cycloheximide, a protein synthesis inhibitor. The up-regulation of GLUT1 protein was accompanied by a significant increased in GLUT1 mRNA expression. ERK1/2 was significantly activated in WETC-treated L6 myotubes. In addition, WETC treatment increased AMPK activity in L6 myotubes.Conclusion: T. crispa increased glucose transport activity of L6 myotubes by enhancing GLUT1 expression, a result of AMPK stimulation.

Effect of Tinospora crispa on glucose uptake in skeletal muscle: role of glucose transporter 1 expression and extracellular signal-regulated kinase1/2 activation

Correspondence: Drug Delivery System Excellence Center, Faculty of Pharmaceutical Sciences, Prince of Songkla University, Songkhla 90110, Thailand

Faculty of Health and Sports Science, Thaksin University, Phatthalung 93110,

Department of Biomedical Sciences, Faculty of Medicine, Prince of Songkla University, Hat-Yai, Songkhla 90110,

Medicinal Plant Research Institute, Department of Medical Science, Ministry of Public Health, Nonthaburi 11000,

School of Medicine, Walailak University, Nakhonsithammarat 80160,

Asian Biomedicine

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 License.

Background: Tinospora crispa (TC) has been used for years as diabetic remedy in Thai-traditional medicine. It has recently been reported that the aqueous...

Effect of Tinospora crispa on glucose uptake in skeletal muscle: role of glucose transporter 1 expression and extracellular signal-regulated kinase1/2 activation

Article Category: Original article

Published Online: Feb 04, 2017

Page range: 361 - 369

DOI: https://doi.org/10.5372/1905-7415.0503.047

Keywords
AMPK, ERK1/2, Glucose uptake, GLUT1, GLUT4, Tinospora crispa

© 2017

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 License.

Effect of Tinospora crispa on glucose uptake in skeletal muscle: role of glucose transporter 1 expression and extracellular signal-regulated kinase1/2 activation

Article Category: Original article

Published Online: Feb 04, 2017

Page range: 361 - 369

DOI: https://doi.org/10.5372/1905-7415.0503.047

KeywordsAMPK, ERK1/2, Glucose uptake, GLUT1, GLUT4, Tinospora crispa

© 2017

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 License.

Keywords
AMPK, ERK1/2, Glucose uptake, GLUT1, GLUT4, Tinospora crispa