This paper presents considerations on selected methodological aspects of respirometric COD fractionation in industrial wastewater which may differ from more common applications of such methodology for municipal wastewater. This concerns: the presence of interfering substances; problems with the elimination of nitrification; the importance of recognising biomass metabolism; differences of growth coefficient; the inclusion of non-canonical COD fractions and toxic effects. The discussion is based on a literature review and the authors’ own experience. As a conclusion, a preparatory procedure is proposed to assure the correct performance of respirometric COD fractionation for industrial wastewater, especially for more complex chemical effluents.