<abstract xmlns="http://www.w3.org/1999/xhtml"><p>The aim of the present study is to determine and to compare the content of retinol, cholecalciferol and alpha-tocopherol in edible tissue of two Black sea fishes - Garfish (<italic>Belone belone</italic>) and Turbot (<italic>Psetta maxima</italic>). All-trans-retinol (vitamin A), cholecalciferol (vitamin D<sub>3</sub>) and alpha-tocopherol (vitamin E) were analyzed simultaneously using HPLC/UV/FL system (Thermo Scientific Spectra SYSTEM) equipped with RP analytical column. The mobile phase was composed of 97:3 = MeOH:H2O. Retinol and cholecalciferol were monitored by UV detection at lmax = 325 nm and lmax = 265 nm, respectively. Alpha-tocopherol was detected by fluorescence at lex=288 nm and lem=332 nm. The sample preparation procedure includes alkaline saponification, followed by liquid-liquid extraction. Quantities of all-trans-retinol and cholecalciferol were higher in garfish tissues while alpha-tocopherol content in turbot showed seven times higher values.</p></abstract>

The aim of the present study is to determine and to compare the content of retinol, cholecalciferol and alpha-tocopherol in edible tissue of two Black sea fishes - Garfish (Belone belone) and Turbot (Psetta maxima). All-trans-retinol (vitamin A), cholecalciferol (vitamin D3) and alpha-tocopherol (vitamin E) were analyzed simultaneously using HPLC/UV/FL system (Thermo Scientific Spectra SYSTEM) equipped with RP analytical column. The mobile phase was composed of 97:3 = MeOH:H2O. Retinol and cholecalciferol were monitored by UV detection at lmax = 325 nm and lmax = 265 nm, respectively. Alpha-tocopherol was detected by fluorescence at lex=288 nm and lem=332 nm. The sample preparation procedure includes alkaline saponification, followed by liquid-liquid extraction. Quantities of all-trans-retinol and cholecalciferol were higher in garfish tissues while alpha-tocopherol content in turbot showed seven times higher values.

Retinol, cholecalciferol and alpha-tocopherol contents of Bulgarian Black Sea fish species

Faculty of Pharmacy, Medical University of Varna, 55 Marin Drinov Str., 9002 Varna, Bulgaria

Ovidius University Annals of Chemistry

The aim of the present study is to determine and to compare the content of retinol, cholecalciferol and alpha-tocopherol in edible tissue of two Black sea...

Retinol, cholecalciferol and alpha-tocopherol contents of Bulgarian Black Sea fish species

Published Online: Mar 22, 2013

Page range: 31 - 34

DOI: https://doi.org/10.2478/v10310-012-0004-7

Keywords
retinol, cholecalciferol, alpha-tocopherol, turbot, garfish, HPLC

This content is open access.

Retinol, cholecalciferol and alpha-tocopherol contents of Bulgarian Black Sea fish species

Published Online: Mar 22, 2013

Page range: 31 - 34

DOI: https://doi.org/10.2478/v10310-012-0004-7

Keywordsretinol, cholecalciferol, alpha-tocopherol, turbot, garfish, HPLC

This content is open access.

Keywords
retinol, cholecalciferol, alpha-tocopherol, turbot, garfish, HPLC