<abstract xml:lang="en" xmlns="http://www.w3.org/1999/xhtml"><p>The effect of the instrumental insemination of honeybee queens after they performed their orientation flight or attempted to perform the flight, on the number of sperm in the spermatheca was observed. Naturally mated queens and instrumentally inseminated queens were examined. Queens were instrumentally inseminated under one of the following 4 circumstances: the instrumentally inseminated queens were either 7 day olds and had been given either a short or long-CO<sub>2</sub> treatment, or they were inseminated after the trial flight or after returning from the orientation flight. Queens from the various groups had a similar number of spermatozoa in their spermatheca (on average, from 4.7 to 5.3 million). The number of spermatozoa filling the spermatheca influenced both the color and the texture of spermathecae. Significant differences in the number of spermatozoa were stated. Instrumentally inseminated queens that did not lay eggs had significantly less spermatozoa in their spermathecae (3.9 mln) than egg laying queens (5.5 mln).</p></abstract>

The effect of the instrumental insemination of honeybee queens after they performed their orientation flight or attempted to perform the flight, on the number of sperm in the spermatheca was observed. Naturally mated queens and instrumentally inseminated queens were examined. Queens were instrumentally inseminated under one of the following 4 circumstances: the instrumentally inseminated queens were either 7 day olds and had been given either a short or long-CO2 treatment, or they were inseminated after the trial flight or after returning from the orientation flight. Queens from the various groups had a similar number of spermatozoa in their spermatheca (on average, from 4.7 to 5.3 million). The number of spermatozoa filling the spermatheca influenced both the color and the texture of spermathecae. Significant differences in the number of spermatozoa were stated. Instrumentally inseminated queens that did not lay eggs had significantly less spermatozoa in their spermathecae (3.9 mln) than egg laying queens (5.5 mln).

Instrumental Insemination of Honey Bee Queens During Flight Activity Predisposition Period 2. Number of Spermatozoa in Spermatheca

Apiculture Division, Research Institute of Horticulture, Kazimierska 2, 24-100 Puławy, Poland

Journal of Apicultural Science

The effect of the instrumental insemination of honeybee queens after they performed their orientation flight or attempted to perform the flight, on the number...

Instrumental Insemination of Honey Bee Queens During Flight Activity Predisposition Period 2. Number of Spermatozoa in Spermatheca

Published Online: Jun 19, 2012

Page range: 159 - 167

DOI: https://doi.org/10.2478/v10289-012-0016-8

Keywords
honeybee, number of sperms, spermatheca, instrumental insemination, flight activity, natural mating, texture and color of spermatheca

This content is open access.

Instrumental Insemination of Honey Bee Queens During Flight Activity Predisposition Period 2. Number of Spermatozoa in Spermatheca

Published Online: Jun 19, 2012

Page range: 159 - 167

DOI: https://doi.org/10.2478/v10289-012-0016-8

Keywordshoneybee, number of sperms, spermatheca, instrumental insemination, flight activity, natural mating, texture and color of spermatheca

This content is open access.

Keywords
honeybee, number of sperms, spermatheca, instrumental insemination, flight activity, natural mating, texture and color of spermatheca