<abstract xmlns="http://www.w3.org/1999/xhtml">

<p> The objective of the study was to determine genotypic profiles of <italic>Bordetella bronchiseptica </italic>(<italic>Bbr</italic>) strains, based on the occurrence of genes encoding virulence factors, such as flagella (<italic>fla</italic>), dermonecrotoxin (<italic>dnt</italic>), and exogenous ferric siderophore receptor (<italic>bfrZ</italic>), using PCR. 209 tested <italic>Bbr </italic>strains were obtained from Polish swine herds with different health status (with progressive atrophic rhinitis - PAR, suspected for PAR, and unknown). In total, seven different <italic>Bbr </italic>genotypes were determined. In 39.2% of <italic>Bbr </italic>isolates all three genes were present. In 41.1% of the isolates only two genes were detected. The most common genotype <italic>dnt</italic>+<italic>bfrZ</italic>-<italic>fla</italic>+ was present in 60 (28.5%) <italic>Bbr </italic>strains, 65% of them were obtained from farms with PAR. Twenty five (12%) <italic>Bbr </italic>isolates were identified as <italic>dnt-bfrZ+fla+ </italic>genotype and, as above, they were more frequently isolated from clinical cases of disease (84%). Among 31 (14.8%) strains only <italic>fla </italic>gene was evident, and in nine (4.3%) only <italic>dnt </italic>gene was present. There were no <italic>Bbr </italic>strains with <italic>bfrZ </italic>gene only. These results confirm the heterogenicity among <italic>Bbr </italic>strains.</p>
</abstract>

The objective of the study was to determine genotypic profiles of Bordetella bronchiseptica (Bbr) strains, based on the occurrence of genes encoding virulence factors, such as flagella (fla), dermonecrotoxin (dnt), and exogenous ferric siderophore receptor (bfrZ), using PCR. 209 tested Bbr strains were obtained from Polish swine herds with different health status (with progressive atrophic rhinitis - PAR, suspected for PAR, and unknown). In total, seven different Bbr genotypes were determined. In 39.2% of Bbr isolates all three genes were present. In 41.1% of the isolates only two genes were detected. The most common genotype dnt+bfrZ-fla+ was present in 60 (28.5%) Bbr strains, 65% of them were obtained from farms with PAR. Twenty five (12%) Bbr isolates were identified as dnt-bfrZ+fla+ genotype and, as above, they were more frequently isolated from clinical cases of disease (84%). Among 31 (14.8%) strains only fla gene was evident, and in nine (4.3%) only dnt gene was present. There were no Bbr strains with bfrZ gene only. These results confirm the heterogenicity among Bbr strains.

Occurrence of Genes Encoding Virulence Factors in Bordetella Bronchiseptica Strains Isolated from Infected and Healthy Pigs

Journal of Veterinary Research

The objective of the study was to determine genotypic profiles of Bordetella bronchiseptica (Bbr) strains, based on the occurrence of genes encoding virulence...

Occurrence of Genes Encoding Virulence Factors in Bordetella Bronchiseptica Strains Isolated from Infected and Healthy Pigs

Published Online: Jan 17, 2013

Page range: 483 - 487

DOI: https://doi.org/10.2478/v10213-012-0085-2

Keywords
swine, atrophic rhinitis, Bordetella bronchiseptica, virulence factors, genes

This content is open access.

Occurrence of Genes Encoding Virulence Factors in Bordetella Bronchiseptica Strains Isolated from Infected and Healthy Pigs

Published Online: Jan 17, 2013

Page range: 483 - 487

DOI: https://doi.org/10.2478/v10213-012-0085-2

Keywordsswine, atrophic rhinitis, Bordetella bronchiseptica, virulence factors, genes

This content is open access.

Keywords
swine, atrophic rhinitis, Bordetella bronchiseptica, virulence factors, genes