<abstract xmlns="http://www.w3.org/1999/xhtml"><p> The aim was to detect antimutagenic and DNA protective effects of a plant extract from bark of Magnolia officinalis adversing oxidative DNA damage. The ability to inhibit mutagenicity induced by tert-butyl hydroperoxide (t-BOOH) and hydrogen peroxide (H2O2) was determined with Ames test using Salmonella typhimurium His- TA102 bacterial strain. Inhibition values of 72.8 and 98.7 % were detected for t-BOOH and H2O2, respectively. The protective effect of the extract against DNA strand scission induced by hydroxyl radicals was studied with plasmid pBluescript II SK(-). The analysis of DNA strand breaks in the plasmid DNA proved a significant inhibition of DNA damage.</p></abstract>

The aim was to detect antimutagenic and DNA protective effects of a plant extract from bark of Magnolia officinalis adversing oxidative DNA damage. The ability to inhibit mutagenicity induced by tert-butyl hydroperoxide (t-BOOH) and hydrogen peroxide (H2O2) was determined with Ames test using Salmonella typhimurium His- TA102 bacterial strain. Inhibition values of 72.8 and 98.7 % were detected for t-BOOH and H2O2, respectively. The protective effect of the extract against DNA strand scission induced by hydroxyl radicals was studied with plasmid pBluescript II SK(-). The analysis of DNA strand breaks in the plasmid DNA proved a significant inhibition of DNA damage.

Antigenotoxic effects of a bark extract from Magnolia officinalis

Department of Biology & Ecology, Faculty of Science, University of Ostrava, Chittussiho 10, 710 00 Ostrava, Czech Republic

Acta Musei Silesiae, Scientiae Naturales

{"article-title":"Antigenotoxic effects of a bark extract from Magnolia officinalis"}

The aim was to detect antimutagenic and DNA protective effects of a plant extract from bark of Magnolia officinalis adversing oxidative DNA damage. The ability...

Antigenotoxic effects of a bark extract from Magnolia officinalis

Published Online: Apr 17, 2013

Page range: 273 - 278

DOI: https://doi.org/10.2478/v10210-012-0028-9

Keywords
Ames test, Antimutagenicity, DNA strand scission, Magnolia officinalis

This content is open access.

Antigenotoxic effects of a bark extract from Magnolia officinalis

Published Online: Apr 17, 2013

Page range: 273 - 278

DOI: https://doi.org/10.2478/v10210-012-0028-9

KeywordsAmes test, Antimutagenicity, DNA strand scission, Magnolia officinalis

This content is open access.

Keywords
Ames test, Antimutagenicity, DNA strand scission, Magnolia officinalis