<abstract xml:lang="en" xmlns="http://www.w3.org/1999/xhtml">Our results show that a subset (miRNA cluster 1) of grapevine (<italic>Vitis vinifera</italic> L., cv. Limberger) microRNAs (miRNAs) can be strongly induced by different apoptosis inducers including methyl jasmonate (MeJA), botrycin, cinerein and/or H2O2. We report here that the expression of endogenous miRNA-g1 and miRNA-g7 can be efficiently silenced in grapevine protoplasts using artificial miRNA (amiRNA) technology. Furthermore, we demonstrate that the ectopic expression of amiRNAs (anti-mir miRNA-g1 and pre-mir miRNA-g1) designed to target a mature miRNA-g1 directs jasmonate-induced silencing against DAD1 protein (defender against death 1). These collective results strongly support the idea that a sub-population of grapevine miRNAs induced by apoptosis may function in one of the most critical defense systems for structural and mechanical fitness.</abstract>

Our results show that a subset (miRNA cluster 1) of grapevine (Vitis vinifera L., cv. Limberger) microRNAs (miRNAs) can be strongly induced by different apoptosis inducers including methyl jasmonate (MeJA), botrycin, cinerein and/or H2O2. We report here that the expression of endogenous miRNA-g1 and miRNA-g7 can be efficiently silenced in grapevine protoplasts using artificial miRNA (amiRNA) technology. Furthermore, we demonstrate that the ectopic expression of amiRNAs (anti-mir miRNA-g1 and pre-mir miRNA-g1) designed to target a mature miRNA-g1 directs jasmonate-induced silencing against DAD1 protein (defender against death 1). These collective results strongly support the idea that a sub-population of grapevine miRNAs induced by apoptosis may function in one of the most critical defense systems for structural and mechanical fitness.

Post-Transcriptional Gene Silencing Conferred by the Ectopic Expression of the Grapevine miRNA-g1 and Inhibition of the Response by Anti-miRNA-g1 Inhibitor

Plant Production Research Center Piešťany

Slovak University of Technology Bratislava

Agriculture (Pol'nohospodárstvo)

Our results show that a subset (miRNA cluster 1) of grapevine (Vitis vinifera L., cv. Limberger) microRNAs (miRNAs) can be strongly induced by different...

Post-Transcriptional Gene Silencing Conferred by the Ectopic Expression of the Grapevine miRNA-g1 and Inhibition of the Response by Anti-miRNA-g1 Inhibitor

Published Online: Dec 25, 2011

Page range: 137 - 143

DOI: https://doi.org/10.2478/v10207-011-0014-z

Keywords
L., small RNA, gene silencing, protoplast, cell death, methyl jasmonate

This content is open access.

Post-Transcriptional Gene Silencing Conferred by the Ectopic Expression of the Grapevine miRNA-g1 and Inhibition of the Response by Anti-miRNA-g1 Inhibitor

Published Online: Dec 25, 2011

Page range: 137 - 143

DOI: https://doi.org/10.2478/v10207-011-0014-z

KeywordsL., small RNA, gene silencing, protoplast, cell death, methyl jasmonate

This content is open access.

Keywords
L., small RNA, gene silencing, protoplast, cell death, methyl jasmonate