<abstract xml:lang="en" xmlns="http://www.w3.org/1999/xhtml"><p>Authors compared the incidence of chromosomal aberrations (CAs) of workers occupationally exposed to cytostatics (group EXP1) or anaesthetics (group EXP2) in relationship to polymorphism of <italic>GSTM1, GSTP1</italic> and <italic>GSTT1</italic> genes. The cytogenetic analysis for chromosomal aberrations frequency and for polymorphisms of genes the PCR and PCR-RFLP method were used. Statistically higher frequency of total CAs was detected in both exposed groups: group EXP1 1.90±1.34%; Mann-Whitney U-test, <italic>p</italic>=0.001; group EXP2 2.53±1.46%, <italic>p</italic>=0.0008) as compared to control (1.26±0.93%). In group EXP2 was detected statistically higher frequency of aberrations CSA-type as compared to CTA-type. In xenobiotic metabolizing genes for GST higher frequency of total CAs and constituent types chromatid-type aberrations (CTAs) and chromosome-type aberrations (CSAs) of genes <italic>GSTM1</italic> and <italic>GSTT1</italic> with null genotype was detected. Statistically significant difference was detected only in CSA-type of aberrations in <italic>GSTT1</italic> gene. In gene <italic>GSTP1</italic> was not detected any difference in frequency of aberrations in presence of the variant allele. Presented results point out importance of individual susceptibility in evaluation of genotoxic agents of anaesthetics or cytostatics.</p></abstract>

Authors compared the incidence of chromosomal aberrations (CAs) of workers occupationally exposed to cytostatics (group EXP1) or anaesthetics (group EXP2) in relationship to polymorphism of GSTM1, GSTP1 and GSTT1 genes. The cytogenetic analysis for chromosomal aberrations frequency and for polymorphisms of genes the PCR and PCR-RFLP method were used. Statistically higher frequency of total CAs was detected in both exposed groups: group EXP1 1.90±1.34%; Mann-Whitney U-test, p=0.001; group EXP2 2.53±1.46%, p=0.0008) as compared to control (1.26±0.93%). In group EXP2 was detected statistically higher frequency of aberrations CSA-type as compared to CTA-type. In xenobiotic metabolizing genes for GST higher frequency of total CAs and constituent types chromatid-type aberrations (CTAs) and chromosome-type aberrations (CSAs) of genes GSTM1 and GSTT1 with null genotype was detected. Statistically significant difference was detected only in CSA-type of aberrations in GSTT1 gene. In gene GSTP1 was not detected any difference in frequency of aberrations in presence of the variant allele. Presented results point out importance of individual susceptibility in evaluation of genotoxic agents of anaesthetics or cytostatics.

Comparison of chromosomal aberrations frequency and polymorphism of GSTs genes in workers occupationally exposed to cytostatics or anaesthetics

Jessenius Faculty of Medicine in Martin, Institute of Medical Biology, Comenius University in Bratislava, Slovakia

Jessenius Faculty of Medicine in Martin, Clinic of Occupational Medicine and Toxicology, Comenius University in Bratislava, Slovakia

Jessenius Faculty of Medicine in Martin, Institute of Medical Biochemistry, Comenius University in Bratislava, Slovakia

National Institute of Public Health in Prague, Czech Republic

Institute of Experimental Medicine Academy of Sciences in Prague, Czech Republic

Jessenius Faculty of Medicine, Institute of Public Health, Comenius University in Bratislava, Slovakia

Interdisciplinary Toxicology

Authors compared the incidence of chromosomal aberrations (CAs) of workers occupationally exposed to cytostatics (group EXP1) or anaesthetics (group EXP2) in...

Comparison of chromosomal aberrations frequency and polymorphism of GSTs genes in workers occupationally exposed to cytostatics or anaesthetics

Published Online: Sep 24, 2009

Page range: 190 - 194

DOI: https://doi.org/10.2478/v10102-009-0016-0

Keywords
chromosomal aberrations, cytostatics, anaesthetics, occupational exposure, polymorphisms of GST genes

This content is open access.

Comparison of chromosomal aberrations frequency and polymorphism of GSTs genes in workers occupationally exposed to cytostatics or anaesthetics

Published Online: Sep 24, 2009

Page range: 190 - 194

DOI: https://doi.org/10.2478/v10102-009-0016-0

Keywordschromosomal aberrations, cytostatics, anaesthetics, occupational exposure, polymorphisms of GST genes

This content is open access.

Keywords
chromosomal aberrations, cytostatics, anaesthetics, occupational exposure, polymorphisms of GST genes