<abstract xml:lang="en" xmlns="http://www.w3.org/1999/xhtml">Non small cell lung cancer (NSCLC) is a highly aggressive malignancy with survival rates limited to some patients in early stages (I and II). Apoptosis resistance is a hallmark of solid tumors that is tightly concerned with their biology. We analyzed the expression of 84 apoptosis-related genes in a group of Bulgarian patients with early-stage NSCLC.RNA samples extracted from 12 early-stage NSCLC patients [five squamous cell carcinomas (SCC) and seven adenocarcinomas (AC)] and eight adjacent non neoplastic pulmonary tissues were used for gene expression analysis. We applied pathway-focused expression profiling of 84 apoptosis-related genes using real-time PCR.Apoptosis-related genes down regulated in NSCLC compared to non tumor lung tissue (<italic>p</italic> &lt;0.05) included representatives of the tumor necrosis factor (TNF) ligand family [TNF superfamily 8 (TNFSF8)], caspase cascade (CASP8 and CASP10) and caspase recruitment domain (CARD) family (BCL10), the positive apoptosis regulator DAPK1 and BCL2 family member MCL1. The potential of apoptosis-related genes as prognostic and predictive markers should be validated in future studies.</abstract>

Non small cell lung cancer (NSCLC) is a highly aggressive malignancy with survival rates limited to some patients in early stages (I and II). Apoptosis resistance is a hallmark of solid tumors that is tightly concerned with their biology. We analyzed the expression of 84 apoptosis-related genes in a group of Bulgarian patients with early-stage NSCLC.RNA samples extracted from 12 early-stage NSCLC patients [five squamous cell carcinomas (SCC) and seven adenocarcinomas (AC)] and eight adjacent non neoplastic pulmonary tissues were used for gene expression analysis. We applied pathway-focused expression profiling of 84 apoptosis-related genes using real-time PCR.Apoptosis-related genes down regulated in NSCLC compared to non tumor lung tissue (p <0.05) included representatives of the tumor necrosis factor (TNF) ligand family [TNF superfamily 8 (TNFSF8)], caspase cascade (CASP8 and CASP10) and caspase recruitment domain (CARD) family (BCL10), the positive apoptosis regulator DAPK1 and BCL2 family member MCL1. The potential of apoptosis-related genes as prognostic and predictive markers should be validated in future studies.

Apoptosis Gene Expression Profile in Early-Stage non Small Cell Lung Cancer

Department of Medical Genetics, Medical University - Sofia, Sofia, Bulgaria

Department of Internal Medicine, Medical University - Sofia, Sofia, Bulgaria

Section for Medical Informatics and Biostatistics, University Department of Social Medicine and Healthcare Management, Medical University - Sofia, Sofia, Bulgaria

Department of Thoracic Surgery, St. Sofia Hospital, Sofia, Bulgaria

Balkan Journal of Medical Genetics

{"article-title":"Apoptosis Gene Expression Profile in Early-Stage non Small Cell Lung Cancer"}

Non small cell lung cancer (NSCLC) is a highly aggressive malignancy with survival rates limited to some patients in early stages (I and II). Apoptosis...

Apoptosis Gene Expression Profile in Early-Stage non Small Cell Lung Cancer

Published Online: Jan 20, 2011

Page range: 47 - 52

DOI: https://doi.org/10.2478/v10034-010-0026-4

Keywords
Apoptosis, Early-stage, Non small cell lung cancer (NSCLC), Expression

This content is open access.

Apoptosis Gene Expression Profile in Early-Stage non Small Cell Lung Cancer

Published Online: Jan 20, 2011

Page range: 47 - 52

DOI: https://doi.org/10.2478/v10034-010-0026-4

KeywordsApoptosis, Early-stage, Non small cell lung cancer (NSCLC), Expression

This content is open access.

Keywords
Apoptosis, Early-stage, Non small cell lung cancer (NSCLC), Expression