Cell size dynamics and viability of cells exposed to hypotonic treatment and electroporation for electrofusion optimization

Chang DC, Chassy BM, Saunders JA, Sowers AE. Guide to Electroporation and Electrofusion. San Diego: Academic Press; 1992.10.1016/B978-0-12-168040-4.50004-3Search in Google Scholar

Hui SW, Stenger DA. Electrofusion of cells: Hybridoma production by electrofusion and polyethylene glycol. Method Enzymol 1993; 220: 212-27.10.1016/0076-6879(93)20084-GSearch in Google Scholar

Zimmermann U, Friedrich U, Mussauer H, Gessner P, Hämel K, Sukhorukov V. Electromanipulation of mammalian cells: Fundamentals and application. IEEE IEEE Trans Plasma Sci 2000; 28: 72-82.10.1109/27.842868Search in Google Scholar

Zimmermann U. Electric field-mediated fusion and related electrical phenomena. Biochim Biophys Acta 1982; 694: 227-77.10.1016/0304-4157(82)90007-7Search in Google Scholar

Teissie J, Rols MP. Fusion of mammalian cells in culture is obtained by creating the contact between cells after their electropermeabilization. Biochem Biophys Res Commun 1986; 140: 258-66.10.1016/0006-291X(86)91084-3Search in Google Scholar

Neumann E, Kakorin S. Digression on membrane electroporation and electroporative delivery of drugs and genes. Radiol Oncol 1998; 32: 7-17.Search in Google Scholar

Rudolf Z, Stabuc R, Cemazar M, Miklavcic D, Vodovnik L, Sersa G. Electrochemotherapy with bleomycin. The first clinical experience in malignant melanoma patients. Radiol Oncol 1995; 29: 229-35.Search in Google Scholar

Cemazar M, Miklavcic D, Vodovnik L, Jarm T, Rudolf Z, Stabuc R, et al. Improved therapeutic effect of electrochemotherapy with cisplatin by intratumoral drug administration and changing of electrode orientation for electropermeabilization on EAT tumor model in mice. Radiol Oncol 1995; 29: 121-7.Search in Google Scholar

Sersa G, Cemazar M, Miklavcic D. Tumor blood flow modifying effects of electrochemotherapy: a potential vascular targeted mechanism. Radiol Oncol 2003; 37: 43-8.Search in Google Scholar

Sersa G, Cemazar M, Miklavcic D, Rudolf Z. Electrochemotherapy of tumours. Radiol Oncol 2006; 40: 163-74.Search in Google Scholar

Pavselj N, Miklavcic D. Numerical modeling in electroporation-based biomedical applications. Radiol Oncol 2008; 42: 159-68.10.2478/v10019-008-0008-2Search in Google Scholar

Zupanic A, Corovic S, Miklavcic D. Optimization of electrode position and electric pulse amplitude in electrochemotherapy. Radiol Oncol 2008; 42: 93-101.10.2478/v10019-008-0005-5Search in Google Scholar

Tsong TY. Electroporation of cell membranes. Biophys J 1991; 60: 297-306.10.1016/S0006-3495(91)82054-9Search in Google Scholar

Teissié J, Rols M. An experimental evaluation of the critical potential difference inducing cell membrane electropermeabilization. Biophys J 1993; 65: 409-413.10.1016/S0006-3495(93)81052-XSearch in Google Scholar

Weaver JC, Chizmadzhev YA. Theory of electroporation: A review. Bioelectrochem Bioener 1996; 41: 135-60.10.1016/S0302-4598(96)05062-3Search in Google Scholar

Neumann E, Sowers AE, Jordan CA. Electroporation and electrofusion in cell biology. New York: Plenum Press; 1989.10.1007/978-1-4899-2528-2Search in Google Scholar

Cemazar M, Jarm T, Miklavcic D, Macek-Lebar A, Ihan A, Kopitar NA, et al. Effect of electric-field intensity on electropermeabilization and electrosensitivity of various tumor-cell lines in vitro. Electro Magnetobiol 1998; 17: 263-72.10.3109/15368379809022571Search in Google Scholar

Canatella PJ, Karr JF, Petros JA, Prausnitz MR. Quantitative study of electroporation-mediated molecular uptake and cell viability. Biophys J 2001; 80: 755-64.10.1016/S0006-3495(01)76055-9Search in Google Scholar

Macek-Lebar A, Miklavcic D. Cell electropermeabilization to small molecules in vitro: control by pulse parameters. Radiol Oncol 2001; 35: 193-202.Search in Google Scholar

Cegovnik U, Novakovic S. Setting optimal parameters for in vitro electrotransfection of B16F1, SA1, LPB, SCK, L929 and CHO cells using predefined exponentially decaying electric pulses. Bioelectrochemistry 2004; 62: 73-82.10.1016/j.bioelechem.2003.10.009Search in Google Scholar

Kanduser M, Sentjurc M, Miklavcic D. Cell membrane fluidity related to electroporation and resealing. Eur Biophys J 2006; 35: 196-204.10.1007/s00249-005-0021-ySearch in Google Scholar

Vienken J, Zimmermann U. An improved electrofusion technique for production of mouse hybridoma cells. FEBS Lett 1985; 182: 278-80.10.1016/0014-5793(85)80315-XSearch in Google Scholar

Mally MI, McKnight ME, Glassy MC. Protocols of electroporation and electrofusion for producing human hybridomas. In: Chang D, Chassy B, Saunders J, Sowers A, editors. Guide to Electroporation and Electrofusion. San Diego: Academic Press; 1992. p. 507-22.10.1016/B978-0-12-168040-4.50035-3Search in Google Scholar

Scott-Taylor TH, Pettengell R, Clarke I, Stuhler G, La Barthe MC, Walden P, et al. Human tumour and dendritic cell hybrids generated by electrofusion: potential for cancer vaccines. Biochim Biophys Acta 2000; 1500: 265-79.10.1016/S0925-4439(99)00108-8Search in Google Scholar

Hayashi T, Tanaka H, Tanaka J, Wang R, Averbook BJ, Cohen PA, et al. Immunogenicity and therapeutic efficacy of dendritic-tumor hybrid cells generated by electrofusion. Clin Immunol 2002; 104: 4-20.10.1006/clim.2002.522412139943Search in Google Scholar

Yu X, McGrawa PA, House FS, Crowe JE Jr. An optimized electrofusion-based protocol for generating virus-specific human monoclonal antibodies. J Immunol Methods 2008; 336: 142-51.10.1016/j.jim.2008.04.008251911718514220Search in Google Scholar

Ahkong QF, Lucy JA. Osmotic forces in artificially induced cell fusion. Biochim Biophys Acta 1986; 858: 206-16.10.1016/0005-2736(86)90308-1Search in Google Scholar

Schmitt JJ, Zimmermann U. Enhanced hybridoma production by electrofusion in strongly hypo-osmolar solutions. Biochim Biophys Acta 1989; 983: 42-50.10.1016/0005-2736(89)90378-7Search in Google Scholar

Zimmermann U, Gessner P, Schnettler R, Perkins S, Foung SK. Efficient hybridization of mouse-human cell lines by means of hypoosmolar electrofusion. J Immunol Methods 1990; 134: 43-50.10.1016/0022-1759(90)90110-HSearch in Google Scholar

Foung S, Perkins S, Kafadar K, Gessner P, Zimmermann U. Development of microfusion techniques to generate human hybridomas. J Immunol Methods 1990; 134: 35-42.10.1016/0022-1759(90)90109-9Search in Google Scholar

Rols MP, Teissié J. Modulation of electrically induced permeabilization and fusion of Chinese hamster ovary cells by osmotic pressure. Biochemistry 1990; 29: 4561-7.10.1021/bi00471a0092372540Search in Google Scholar

Rehman SMM, Perkins S, Zimmermann U, Foung SKH. Human hybridoma formation by hypo-osmolar electrofusion. In: Chang D, Chassy B, Saunders J, Sowers A, editors. Guide to Electroporation and Electrofusion. Academic Press; 1992. p. 523-33.10.1016/B978-0-12-168040-4.50036-5Search in Google Scholar

Sukhorukov VL, Reuss R, Zimmermann D, Held C, Müller KJ, Kiesel M, et al. Surviving high-intensity field pulses: Strategies for improving robustness and performance of electrotransfection and electrofusion. J Membr Biol 2005; 206: 187-201.10.1007/s00232-005-0791-216456714Search in Google Scholar

Sukhorukov VL, Reuss R, Endter JM, Fehrmann S, Katsen-Globa A, Geßner P, et al. A biophysical approach to the optimisation of dendritictumour cell electrofusion. Biochem Biophys Res Commun 2006; 346: 829-39.10.1016/j.bbrc.2006.05.19316780801Search in Google Scholar

Kiesel M, Reuss R, Zimmermann D, Zimmermann H, Shirakashi R, Bamberg E, et al. Biophys J 2006; 90: 4720-9.10.1529/biophysj.105.078725147185616565059Search in Google Scholar

Pavlin M, Kanduser M, Rebersek M, Pucihar G, Hart FX, Magjarevic R, et al. Effect of cell electroporation on the conductivity of a cell suspension. Biophys J 2005; 88: 4378-439.10.1529/biophysj.104.048975130566515792975Search in Google Scholar

Gillies RJ, Didier N, Denton M. Determination of cell number in monolayer cultures. Anal Biochem 1986; 159: 109-13.10.1016/0003-2697(86)90314-3Search in Google Scholar

Sarkadi B, Attisano L, Grinstein S, Buchwald M, Rothstein A. Volume regulation of Chinese hamster ovary cells in anisoosmotic buffer. Biochim Biophys Acta 1984; 774: 159-68.10.1016/0005-2736(84)90287-6Search in Google Scholar

Barrau C, Teissié J, Gabriel B. Osmotically induced membrane tension facilitates the triggering of living cell electropermeabilization. Bioelectrochemistry 2004; 63: 327-32.10.1016/j.bioelechem.2003.11.009Search in Google Scholar

Reuss R, Ludwig J, Shirakashi R, Ehrhart F, Zimmermann H, Schneider S, et al. Intracellular delivery of carbohydrates into mammalian cells through swelling-activated pathways. J Membr Biol 2004; 200: 67-81.10.1007/s00232-004-0694-7Search in Google Scholar

Zimmermann D, Terpitz U, Zhou A, Reuss R, Müller K, Sukhorukov VL, et al. Biophysical characterisation of electrofused giant HEK293-cells as a novel electrophysiological expression system. Biochem Biophys Res Commun 2006; 348: 673-81.10.1016/j.bbrc.2006.07.112Search in Google Scholar

Knutton S, Jackson D, Graham JM, Micklem KJ, Pasternak CA. Microvilli and cell swelling. Nature 1976; 262: 52-4.10.1038/262052a0Search in Google Scholar

Sukhorukov VL, Arnold WM, Zimmermann U. Hypotonically induced changes in the plasma membrane of cultured mammalian cells. J Membr Biol 1993; 132: 27-40.10.1007/BF00233049Search in Google Scholar

Evans EA, Parsegian VA. Thermal-mechanical fluctuations enhance repulsion between bimolecular layers. Proc Natl Acad Sci USA 1986; 83: 7132-6.10.1073/pnas.83.19.7132Search in Google Scholar

McIntosh TJ, Advani S, Burton RE, Zhelev DV, Needham D, Simon SA. Experimental test for protrusion and undulation pressures in phospholipid bilayers. Biochemistry 1995; 34: 8520-32.10.1021/bi00027a002Search in Google Scholar

McIntosh TJ, Kulkarni KG, Simon SA. Membrane fusion promoters and inhibitors have contrasting effects on lipid bilayer structure and undulations. Biophys J 1999; 76: 2090-8.10.1016/S0006-3495(99)77365-0Search in Google Scholar

Golzio M, Mora MP, Raynaud C, Delteil C, Teissié J, Rols MP. Control by osmotic pressure of voltage-induced permeabilization and gene transfer in mammalian cells. Biophys J 1998; 74: 3015-22.10.1016/S0006-3495(98)78009-9Search in Google Scholar

Siebens AW, Spring KR. A novel sorbitol transport mechanism in cultured renal papillary epithelial cells. Am J Physiol Cell Physiol 1989; 257(6 Pt 2): F937-46.10.1152/ajprenal.1989.257.6.F937Search in Google Scholar

Hall JA, Kirk J, Potts JR, Rae C Kirk K. Anion channel blockers inhibit swelling-activated anion, cation, and nonelectrolyte transport in HeLa cells. Am J Physiol Cell Physiol 1996; 271: 579-88.10.1152/ajpcell.1996.271.2.C579Search in Google Scholar

Neil GA, Zimmermann U. Electrofusion. Methods Enzymol 1993; 220: 174.10.1016/0076-6879(93)20082-ESearch in Google Scholar

Zimmermann U, Neil GA. Electromanipulation of Cells. Boca Raton: CRC Press; 1995.Search in Google Scholar

Kotnik T, Bobanovic F, Miklavcic D. Sensitivity of transmembrane voltage induced by applied electric fields - a theoretical analysis. Bioelectrochem Bioener 1997; 43: 285-91.10.1016/S0302-4598(97)00023-8Search in Google Scholar