Evaluation of shRNA-mediated gene silencing by electroporation in LPB fibrosarcoma cells

Rols MP, Delteil C, Golzio M, Dumond P, Cros S, Teissie J. In vivo electrically mediated protein and gene transfer in murine melanoma. Nat Biotechnol 1998; 16(2): 168-71.10.1038/nbt0298-168Search in Google Scholar

Wells JM, Li LH, Sen A, Jahreis GP, Hui SW. Electroporation-enhanced gene delivery in mammary tumors. Gene Ther 2000; 7(7): 541-7.10.1038/sj.gt.3301141Search in Google Scholar

Bettan M, Ivanov MA, Mir LM, Boissiere F, Delaere P, Scherman D. Efficient DNA electrotransfer into tumors. Bioelectrochemistry 2000; 52(1): 83-90.10.1016/S0302-4598(00)00087-8Search in Google Scholar

Cemazar M, Sersa G, Wilson J, Tozer GM, Hart SL, Grosel A et al. Effective gene transfer to solid tumors using different nonviral gene delivery techniques: electroporation, liposomes, and integrin-targeted vector. Cancer Gene Ther 2002; 9(4): 399-406.10.1038/sj.cgt.770045411960291Search in Google Scholar

Cemazar M, Wilson I, Dachs GU, Tozer GM, Sersa G. Direct visualization of electroporation-assisted in vivo gene delivery to tumors using intravital microscopy - spatial and time dependent distribution. BMC Cancer 2004; 4(81.)10.1186/1471-2407-4-8153410415546484Search in Google Scholar

Izquierdo M. Short interfering RNAs as a tool for cancer gene therapy. Cancer Gene Ther 2005; 12(3): 217-27.10.1038/sj.cgt.770079115550938Search in Google Scholar

Scherer L, Rossi JJ. Recent applications of RNAi in mammalian systems. Curr Pharm Biotechnol 2004; 5(4): 355-60.10.2174/138920104337672415320766Search in Google Scholar

Pai SI, Lin YY, Macaes B, Meneshian A, Hung CF, Wu TC. Prospects of RNA interference therapy for cancer. Gene Ther 2006; 13(6): 464-77.10.1038/sj.gt.330269416341059Search in Google Scholar

Dykxhoorn DM, Palliser D, Lieberman J. The silent treatment: siRNAs as small molecule drugs. Gene Ther 2006; 13(6): 541-52.10.1038/sj.gt.330270316397510Search in Google Scholar

McAnuff MA, Rettig GR, Rice KG. Potency of siRNA versus shRNA mediated knockdown in vivo. J Pharm Sci 2007; 96(11): 2922-30.10.1002/jps.2096817518360Search in Google Scholar

Gottesman MM. Cancer gene therapy: an awkward adolescence. Cancer Gene Ther 2003; 10(7): 501-8.10.1038/sj.cgt.770060212833130Search in Google Scholar

Meyer M, Wagner E. Recent developments in the application of plasmid DNA-based vectors and small interfering RNA therapeutics for cancer. Hum Gene Ther 2006; 17(11): 1062-76.10.1089/hum.2006.17.106217032153Search in Google Scholar

Grassi G, Maccaroni P, Meyer R, Kaiser H, D'Ambrosio E, Pascale E et al. Inhibitors of DNA methylation and histone deacetylation activate cytomegalovirus promoter-controlled reporter gene expression in human glioblastoma cell line U87. Carcinogenesis 2003; 24(10): 1625-35.10.1093/carcin/bgg11812869421Search in Google Scholar

Krishnan M, Park JM, Cao F, Wang D, Paulmurugan R, Tseng JR et al. Effects of epigenetic modulation on reporter gene expression: implications for stem cell imaging. FASEB J 2006; 20(1): 106-8.10.1096/fj.05-4551fje362542416246867Search in Google Scholar

Liu HS, Jan MS, Chou CK, Chen PH, Ke NJ. Is green fluorescent protein toxic to the living cells? Biochem Biophys Res Commun 1999; 260(3): 712-7.10.1006/bbrc.1999.095410403831Search in Google Scholar

Jana S, Chakraborty C, Nandi S, Deb JK. RNA interference: potential therapeutic targets. Appl Microbiol Biotechnol 2004; 65(6): 649-57.10.1007/s00253-004-1732-115372214Search in Google Scholar

Bantounas I, Phylactou LA, Uney JB. RNA interference and the use of small interfering RNA to study gene function in mammalian systems. J Mol Endocrinol 2004; 33(3): 545-57.10.1677/jme.1.0158215591019Search in Google Scholar

Behlke MA. Progress towards in vivo use of siRNAs. Mol Ther 2006; 13(4): 644-70.10.1016/j.ymthe.2006.01.001710628616481219Search in Google Scholar

Birmingham A, Anderson E, Sullivan K, Reynolds A, Boese Q, Leake D et al. A protocol for designing siRNAs with high functionality and specificity. Nat Protoc 2007; 2(9): 2068-78.10.1038/nprot.2007.27817853862Search in Google Scholar

Paddison PJ, Caudy AA, Bernstein E, Hannon GJ, Conklin DS. Short hairpin RNAs (shRNAs) induce sequence-specific silencing in mammalian cells. Genes Dev 2002; 16(8): 948-58.10.1101/gad.98100215235211959843Search in Google Scholar

Brummelkamp TR, Bernards R, Agami R. A system for stable expression of short interfering RNAs in mammalian cells. Science 2002; 296(5567): 550-3.10.1126/science.1068999Search in Google Scholar

Cullen BR. Induction of stable RNA interference in mammalian cells. Gene Ther 2006; 13(6): 503-8.10.1038/sj.gt.3302656Search in Google Scholar

McManus MT, Petersen CP, Haines BB, Chen J, Sharp PA. Gene silencing using micro-RNA designed hairpins. RNA 2002; 8(6): 842-50.10.1017/S1355838202024032Search in Google Scholar

Xie FY, Woodle MC, Lu PY. Harnessing in vivo siRNA delivery for drug discovery and therapeutic development. Drug Discov Today 2006; 11(1-2): 67-73.10.1016/S1359-6446(05)03668-8Search in Google Scholar

Russ V, Wagner E. Cell and tissue targeting of nucleic acids for cancer gene therapy. Pharm Res 2007; 24(6): 1047-57.10.1007/s11095-006-9233-917387604Search in Google Scholar

Sersa G, Cemazar M, Miklavcic D, Rudolf Z. Electrochemotherapy of tumours. Radiol Oncol 2006; 40(3): 163-74.Search in Google Scholar

Heller LC, Heller R. In vivo electroporation for gene therapy. Hum Gene Ther 2006; 17(9): 890-7.10.1089/hum.2006.17.89016972757Search in Google Scholar

Tamura T, Sakata T. Application of in vivo electroporation to cancer gene therapy. Curr Gene Ther 2003; 3(1): 59-64.10.2174/156652303334746212553536Search in Google Scholar

Cemazar M, Sersa G. Electrotransfer of therapeutic molecules into tissues. Curr Opin Mol Ther 2007; 9(6): 554-62.Search in Google Scholar

Fan Y, Xin XY, Chen BL, Ma X. Knockdown of RAB25 expression by RNAi inhibits growth of human epithelial ovarian cancer cells in vitro and in vivo. Pathology 2006; 38(6): 561-7.Search in Google Scholar

Nakai N, Kishida T, Shin-Ya M, Imanishi J, Ueda Y, Kishimoto S et al. Therapeutic RNA interference of malignant melanoma by electrotransfer of small interfering RNA targeting Mitf. Gene Ther 2007; 14(4): 357-65.10.1038/sj.gt.330286817024102Search in Google Scholar

Takei Y, Nemoto T, Mu P, Fujishima T, Ishimoto T, Hayakawa Y et al. In vivo silencing of a molecular target by short interfering RNA electroporation: tumor vascularization correlates to delivery efficiency. Mol Cancer Ther 2008; 7(1): 211-21.10.1158/1535-7163.MCT-07-031918202023Search in Google Scholar

Golzio M, Mazzolini L, Ledoux A, Paganin A, Izard M, Hellaudais L et al. In vivo gene silencing in solid tumors by targeted electrically mediated siRNA delivery. Gene Ther 2007; 14(9): 752-9.10.1038/sj.gt.330292017344906Search in Google Scholar

Takahashi Y, Nishikawa M, Kobayashi N, Takakura Y. Gene silencing in primary and metastatic tumors by small interfering RNA delivery in mice: quantitative analysis using melanoma cells expressing firefly and sea pansy luciferases. J Control Release 2005; 105(3): 332-43.10.1016/j.jconrel.2005.04.012Search in Google Scholar

Takahashi Y, Nishikawa M, Takakura Y. Suppression of tumor growth by intratumoral injection of short hairpin RNA-expressing plasmid DNA targeting beta-catenin or hypoxia-inducible factor 1alpha. J Control Release 2006; 116(1): 90-5.10.1016/j.jconrel.2006.09.002Search in Google Scholar

Rejiba S, Wack S, Aprahamian M, Hajri A. K-ras oncogene silencing strategy reduces tumor growth and enhances gemcitabine chemotherapy efficacy for pancreatic cancer treatment. Cancer Sci 2007; 98(7): 1128-36.10.1111/j.1349-7006.2007.00506.xSearch in Google Scholar

Chiu YL, Rana TM. RNAi in human cells: basic structural and functional features of small interfering RNA. Mol Cell 2002; 10(3): 549-61.10.1016/S1097-2765(02)00652-4Search in Google Scholar

Devi GR. siRNA-based approaches in cancer therapy. Cancer Gene Ther 2006; 13(9): 819-29.10.1038/sj.cgt.770093116424918Search in Google Scholar

Thomas M, Lu JJ, Ge Q, Zhang C, Chen J, Klibanov AM. Full deacylation of polyethylenimine dramatically boosts its gene delivery efficiency and specificity to mouse lung. Proc Natl Acad Sci U S A 2005; 102(16): 5679-84.10.1073/pnas.050206710255630215824322Search in Google Scholar

Grayson AC, Doody AM, Putnam D. Biophysical and structural characterization of polyethylenimine-mediated siRNA delivery in vitro. Pharm Res 2006; 23(8): 1868-76.10.1007/s11095-006-9009-216845585Search in Google Scholar

Merkerova M, Klamova H, Brdicka R, Bruchova H. Targeting of gene expression by siRNA in CML primary cells. Mol Biol Rep 2007; 34(1): 27-33.10.1007/s11033-006-9006-x17094012Search in Google Scholar

Bartlett DW, Davis ME. Insights into the kinetics of siRNA-mediated gene silencing from live-cell and live-animal bioluminescent imaging. Nucleic Acids Res 2006; 34(1): 322-33.10.1093/nar/gkj439133199416410612Search in Google Scholar

Corish P, Tyler-Smith C. Attenuation of green fluorescent protein half-life in mammalian cells. Protein Eng 1999; 12(12): 1035-40.10.1093/protein/12.12.103510611396Search in Google Scholar

Agami R. RNAi and related mechanisms and their potential use for therapy. Curr Opin Chem Biol 2002; 6(6): 829-34.10.1016/S1367-5931(02)00378-2Search in Google Scholar

Caplen NJ. Gene therapy progress and prospects. Downregulating gene expression: the impact of RNA interference. Gene Ther 2004; 11(16): 1241-8.10.1038/sj.gt.330232415292914Search in Google Scholar

Ryther RC, Flynt AS, Phillips JA, III, Patton JG. siRNA therapeutics: big potential from small RNAs. Gene Ther 2005; 12(1): 5-11.10.1038/sj.gt.330235615496962Search in Google Scholar