<abstract xml:lang="en" xmlns="http://www.w3.org/1999/xhtml"><p>Application of a sensitive and rapid flow injection analysis (FIA) method for determination of topiramate, piracetam, and levetiracetam in pharmaceutical formulations has been investigated. The method is based on the reaction with <italic>ortho</italic>-phtalaldehyde and 2-mercaptoethanol in a basic buffer and measurement of absorbance at 295 nm under flow conditions. Variables affecting the determination such as sample injection volume, pH, ionic strength, reagent concentrations, flow rate of reagent and other FIA parameters were optimized to produce the most sensitive and reproducible results using a quarter-fraction factorial design, for five factors at two levels. Also, the method has been optimized and fully validated in terms of linearity and range, limit of detection and quantitation, precision, selectivity and accuracy. The method was successfully applied to the analysis of pharmaceutical preparations.</p></abstract>

Application of a sensitive and rapid flow injection analysis (FIA) method for determination of topiramate, piracetam, and levetiracetam in pharmaceutical formulations has been investigated. The method is based on the reaction with ortho-phtalaldehyde and 2-mercaptoethanol in a basic buffer and measurement of absorbance at 295 nm under flow conditions. Variables affecting the determination such as sample injection volume, pH, ionic strength, reagent concentrations, flow rate of reagent and other FIA parameters were optimized to produce the most sensitive and reproducible results using a quarter-fraction factorial design, for five factors at two levels. Also, the method has been optimized and fully validated in terms of linearity and range, limit of detection and quantitation, precision, selectivity and accuracy. The method was successfully applied to the analysis of pharmaceutical preparations.

Optimized and validated flow-injection spectrophotometric analysis of topiramate, piracetam and levetiracetam in pharmaceutical formulations

Pharmaceutical Analytical Chemistry Department, Faculty of Pharmacy, University of Suez Canal, Ismailia 41522, Egypt

Pharmaceutical Analytical Chemistry Department, Faculty of Pharmacy, Misr International University, Egypt

Acta Pharmaceutica

Application of a sensitive and rapid flow injection analysis (FIA) method for determination of topiramate, piracetam, and levetiracetam in pharmaceutical...

Optimized and validated flow-injection spectrophotometric analysis of topiramate, piracetam and levetiracetam in pharmaceutical formulations

Published Online: Dec 25, 2011

Page range: 377 - 389

DOI: https://doi.org/10.2478/v10007-011-0038-y

Keywords
topiramat, piracetam, levetiracetam, protočna injekcijska analiza

This content is open access.

Optimized and validated flow-injection spectrophotometric analysis of topiramate, piracetam and levetiracetam in pharmaceutical formulations

Published Online: Dec 25, 2011

Page range: 377 - 389

DOI: https://doi.org/10.2478/v10007-011-0038-y

Keywordstopiramat, piracetam, levetiracetam, protočna injekcijska analiza

This content is open access.

Keywords
topiramat, piracetam, levetiracetam, protočna injekcijska analiza