<abstract xml:lang="en" xmlns="http://www.w3.org/1999/xhtml">β-Human chorionic gonadotropin (β-hCG) was successively labeled with [67Ga]-gallium chloride after conjugation with freshly prepared diethylenetriaminepentaacetic acid dianhydride (ccDTPA). After solid phase purification of the radiolabeled hormone, high performance liquid chromatography showed radiochemical purity higher than 95 % under optimized conditions (specific activity = 22-23 TBq mM-1, labeling efficiency 80 %). Preliminary <italic>in vivo</italic> studies (ID g-1, %) in male wild-type rats showed marked gonadal uptake of the tracer after 240 minutes in agreement with the biodistribution studies and reported β-hCG receptors. Target to blood ratios were 5.1 and 15.2 after 3 and 24 hours, respectively, while target to muscle ratios were 35 and 40 after 3 and 24 hours, respectively.</abstract>

β-Human chorionic gonadotropin (β-hCG) was successively labeled with [67Ga]-gallium chloride after conjugation with freshly prepared diethylenetriaminepentaacetic acid dianhydride (ccDTPA). After solid phase purification of the radiolabeled hormone, high performance liquid chromatography showed radiochemical purity higher than 95 % under optimized conditions (specific activity = 22-23 TBq mM-1, labeling efficiency 80 %). Preliminary in vivo studies (ID g-1, %) in male wild-type rats showed marked gonadal uptake of the tracer after 240 minutes in agreement with the biodistribution studies and reported β-hCG receptors. Target to blood ratios were 5.1 and 15.2 after 3 and 24 hours, respectively, while target to muscle ratios were 35 and 40 after 3 and 24 hours, respectively.

Development of a radiolabeled β-human chorionic gonadotropin

Nuclear Medicine Research Group Agricultural Medical and Industrial, Research School (AMIRS-NSTRI), Karaj, Iran

Islamic Azad University, Science and Research, Branch P. O. Box 14155-775, Tehran, Iran

Islamic Azad University, Young Researcher Club Takestan, Branch, Iran

Acta Pharmaceutica

{"article-title":"Development of a radiolabeled β-human chorionic gonadotropin"}

β-Human chorionic gonadotropin (β-hCG) was successively labeled with [67Ga]-gallium chloride after conjugation with freshly prepared...

Development of a radiolabeled β-human chorionic gonadotropin

Published Online: Nov 16, 2009

Page range: 421 - 429

DOI: https://doi.org/10.2478/v10007-009-0035-6

Keywords
β-hCG, radiomarkiranje, biodistribucija, radiofarmaka

This content is open access.

Development of a radiolabeled β-human chorionic gonadotropin

Published Online: Nov 16, 2009

Page range: 421 - 429

DOI: https://doi.org/10.2478/v10007-009-0035-6

Keywordsβ-hCG, radiomarkiranje, biodistribucija, radiofarmaka

This content is open access.

Keywords
β-hCG, radiomarkiranje, biodistribucija, radiofarmaka