<abstract xml:lang="en" xmlns="http://www.w3.org/1999/xhtml">Two spectrophotometric methods for the determination of ceftazidime (CFZM) in either pure form or in its pharmaceutical formulations are described. The first method is based on the reaction of 3-methylbenzothiazolin-2-one hydrazone (MBTH) with ceftazidime in the presence of ferric chloride in acidic medium. The resulting blue complex absorbs at λmax 628 nm. The second method describes the reaction between the diazotized drug and <italic>N</italic>-(1-naphthyl)ethylenediamine dihydrochloride (NEDA) to yield a purple colored product with λmax at 567 nm. The reaction conditions were optimized to obtain maximum color intensity. The absorbance was found to increase linearly with increasing the concentration of CFZM; the systems obeyed the Beer's law in the range 2-10 and 10-50 μg mL-1 for MBTH and NEDA methods, resp. <italic>LOD, LOQ</italic> and correlation coefficient values were 0.15, 0.79 and 0.50, 2.61. No interference was observed from common excipients present in pharmaceutical formulations. The proposed methods are simple, sensitive, accurate and suitable for quality control applications.</abstract>

Two spectrophotometric methods for the determination of ceftazidime (CFZM) in either pure form or in its pharmaceutical formulations are described. The first method is based on the reaction of 3-methylbenzothiazolin-2-one hydrazone (MBTH) with ceftazidime in the presence of ferric chloride in acidic medium. The resulting blue complex absorbs at λmax 628 nm. The second method describes the reaction between the diazotized drug and N-(1-naphthyl)ethylenediamine dihydrochloride (NEDA) to yield a purple colored product with λmax at 567 nm. The reaction conditions were optimized to obtain maximum color intensity. The absorbance was found to increase linearly with increasing the concentration of CFZM; the systems obeyed the Beer's law in the range 2-10 and 10-50 μg mL-1 for MBTH and NEDA methods, resp. LOD, LOQ and correlation coefficient values were 0.15, 0.79 and 0.50, 2.61. No interference was observed from common excipients present in pharmaceutical formulations. The proposed methods are simple, sensitive, accurate and suitable for quality control applications.

Development and validation of spectrophotometric methods for determination of ceftazidime in pharmaceutical dosage forms

Department of Chemistry, Gulbarga University, Gulbarga-585106, India

Acta Pharmaceutica

Two spectrophotometric methods for the determination of ceftazidime (CFZM) in either pure form or in its pharmaceutical formulations are described. The first...

Development and validation of spectrophotometric methods for determination of ceftazidime in pharmaceutical dosage forms

Published Online: Dec 22, 2008

Page range: 275 - 285

DOI: https://doi.org/10.2478/v10007-008-0017-0

Keywords
ceftazidim, diazotacija, spektrofotometrija, farmaceutski pripravak

This content is open access.

Development and validation of spectrophotometric methods for determination of ceftazidime in pharmaceutical dosage forms

Published Online: Dec 22, 2008

Page range: 275 - 285

DOI: https://doi.org/10.2478/v10007-008-0017-0

Keywordsceftazidim, diazotacija, spektrofotometrija, farmaceutski pripravak

This content is open access.

Keywords
ceftazidim, diazotacija, spektrofotometrija, farmaceutski pripravak