<abstract xmlns="http://www.w3.org/1999/xhtml"><p> Female ageing represents the biological process of structural and functional changes in endocrine cells and tissues, as well as in pituitary hormone-producing cells. In addition to the hypothalamic releasing hormones, estradiol plays a significant role in the regulation of the synthesis/secretion of pituitary hormones and is still used therapeutically for menopausal symptoms. The effects of ageing or ovariectomy and synthetic estradiol application under these circumstances were evaluated in pituitary hormone-producing cells of female rats (animal models of menopause); i.e., the following cells were observed: gonadotropes (FSH and LH), thyrotropes (TSH), somatotropes (GH), mammotropes (PRL) and corticotropes (ACTH). The cells were immunostained and histologically analysed. The ELISA method was used for hormonal analyses. Ageing was found to cause diverse, commonly reductive changes regarding the volume, number and secretion of menopausal rat pituitary hormone- producing cells, except for PRL cells that exhibit significantly increased numbers and intensified secretion. After the treatment of middle-aged female rats wiThestradiol, the absolute and relative pituitary weights significantly increased in comparison with the control females. Histological parameters such as the cell and volume density of PRL and ACThcells were significantly increased compared with the control values. The mentioned parameters of FSH, LH, GH, and occasionally TSH cells after estradiol treatment significantly decreased in comparison with the controls. The corresponding hormone levels followed the changes in the histological parameters. These data indicate that the application of estradiol to menopausal females may specifically, in two directions, modify the histological characteristics and secretory activities of different pituitary-hormone producing cells.. </p></abstract>

Female ageing represents the biological process of structural and functional changes in endocrine cells and tissues, as well as in pituitary hormone-producing cells. In addition to the hypothalamic releasing hormones, estradiol plays a significant role in the regulation of the synthesis/secretion of pituitary hormones and is still used therapeutically for menopausal symptoms. The effects of ageing or ovariectomy and synthetic estradiol application under these circumstances were evaluated in pituitary hormone-producing cells of female rats (animal models of menopause); i.e., the following cells were observed: gonadotropes (FSH and LH), thyrotropes (TSH), somatotropes (GH), mammotropes (PRL) and corticotropes (ACTH). The cells were immunostained and histologically analysed. The ELISA method was used for hormonal analyses. Ageing was found to cause diverse, commonly reductive changes regarding the volume, number and secretion of menopausal rat pituitary hormone- producing cells, except for PRL cells that exhibit significantly increased numbers and intensified secretion. After the treatment of middle-aged female rats wiThestradiol, the absolute and relative pituitary weights significantly increased in comparison with the control females. Histological parameters such as the cell and volume density of PRL and ACThcells were significantly increased compared with the control values. The mentioned parameters of FSH, LH, GH, and occasionally TSH cells after estradiol treatment significantly decreased in comparison with the controls. The corresponding hormone levels followed the changes in the histological parameters. These data indicate that the application of estradiol to menopausal females may specifically, in two directions, modify the histological characteristics and secretory activities of different pituitary-hormone producing cells..

Pituitary Hormone-producing Cells After Estradiol Application in Rat Models of Menopause

Department of Cytology, Institute for Biological Research “Siniša Stanković”, University of Belgrade 142 Despot Stefan Blvd., 11060 Belgrade, Serbia; Tel: +381-11-2078-304; Fax: +381-11-2761-433

Department of Cytology, Institute for Biological Research “Siniša Stanković”, University of Belgrade, 11060 Belgrade, Serbia

Experimental and Applied Biomedical Research (EABR)

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Female ageing represents the biological process of structural and functional changes in endocrine cells and tissues, as well as in pituitary hormone-producing...

Pituitary Hormone-producing Cells After Estradiol Application in Rat Models of Menopause

Published Online: Oct 10, 2014

Page range: 115 - 120

Received: May 15, 2014

Accepted: May 15, 2014

DOI: https://doi.org/10.2478/sjecr-2014-0015

© 2014

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.