<abstract xmlns="http://www.w3.org/1999/xhtml"> Japanese flowering cherry (Prunus subgenus Cerasus; Rosa­ceae) cultivars, which are characterized by beautiful flowers, have been generated through hybridization among wild Pru­nus taxa. The analysis of polymorphisms in the maternally inhe­rited chloroplast DNA is an effective complementary approach for tracing the origins of these cultivars. Thus, a set of novel chloroplast simple sequence repeat (cpSSR) markers was deve­loped for the Prunus taxa, and their utility in cross-species amp­lification was evaluated. Five markers were designed from the partial chloroplast genome sequences of thirteen Prunus taxa. In all, we found 19 haplotypes among the 311 individuals tes­ted. The number of haplotypes, haplotype richness and haplo­type diversity in each taxon ranged from 1 to 8, from 1 to 6.613 and from 0 to 0.758, respectively. The major proportion of the total diversity (HT = 0.831) was accounted for by intraspecific diversity (HS = 0.559), and the level of species subdivision, GST, was 0.327. These five cpSSR markers highly polymorphic, and they will be useful not only for tracing the origins of the culti­vars but also for studying the population structure, diversity, and phylogeography of Prunus.</abstract>

Japanese flowering cherry (Prunus subgenus Cerasus; Rosa­ceae) cultivars, which are characterized by beautiful flowers, have been generated through hybridization among wild Pru­nus taxa. The analysis of polymorphisms in the maternally inhe­rited chloroplast DNA is an effective complementary approach for tracing the origins of these cultivars. Thus, a set of novel chloroplast simple sequence repeat (cpSSR) markers was deve­loped for the Prunus taxa, and their utility in cross-species amp­lification was evaluated. Five markers were designed from the partial chloroplast genome sequences of thirteen Prunus taxa. In all, we found 19 haplotypes among the 311 individuals tes­ted. The number of haplotypes, haplotype richness and haplo­type diversity in each taxon ranged from 1 to 8, from 1 to 6.613 and from 0 to 0.758, respectively. The major proportion of the total diversity (HT = 0.831) was accounted for by intraspecific diversity (HS = 0.559), and the level of species subdivision, GST, was 0.327. These five cpSSR markers highly polymorphic, and they will be useful not only for tracing the origins of the culti­vars but also for studying the population structure, diversity, and phylogeography of Prunus.

Development and characterization of chloroplast simple sequence repeat markers for Prunus taxa (eleven Japanese native taxa and two foreign taxa)

Silvae Genetica

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 License.

Japanese flowering cherry (Prunus subgenus Cerasus; Rosa­ceae) cultivars, which are characterized by beautiful flowers, have been generated through...

Development and characterization of chloroplast simple sequence repeat markers for Prunus taxa (eleven Japanese native taxa and two foreign taxa)

Published Online: Dec 31, 2018

Page range: 124 - 126

DOI: https://doi.org/10.2478/sg-2018-0018

Keywords
Prunus, Cerasus, flowering cherry, cpDNA, SSR, genetic diversity

© 2018 Shuri Kato, et al., published by Sciendo

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 License.

Development and characterization of chloroplast simple sequence repeat markers for Prunus taxa (eleven Japanese native taxa and two foreign taxa)

Published Online: Dec 31, 2018

Page range: 124 - 126

DOI: https://doi.org/10.2478/sg-2018-0018

KeywordsPrunus, Cerasus, flowering cherry, cpDNA, SSR, gene­tic diversity

© 2018 Shuri Kato, et al., published by Sciendo

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 License.

Keywords
Prunus, Cerasus, flowering cherry, cpDNA, SSR, genetic diversity