<abstract xml:lang="en" xmlns="http://www.w3.org/1999/xhtml"><p>Gnathostomiasis is an emerging infectious disease, in which the agent Gnathostoma binucleatum is the only species known to infect humans in Mexico. Although the humoral immune response to G. binucleatum has been analyzed previously, here we evaluated the lymphoproliferative response of peripheral blood lymphocytic cells from patients clinically diagnosed with gnathostomiasis against a crude extract of G. binucleatum using the MTT method, and compared the response to that of healthy subjects. No differences in the response to concanavalin A were found between the groups; however, the response to the crude extract was statistically higher in patients compared to healthy controls (1.188 ± 0.135, and 1.000 ± 0.045, p = 0.0004). Patients at > 6 months after diagnosis showed a higher lymphoproliferative response than the group of healthy subjects, and those at &lt; 6 months after diagnosis (p = 0.0001, and 0.0164 respectively). These data suggest that the parasite induces non-protective immunological memory.</p></abstract>

Gnathostomiasis is an emerging infectious disease, in which the agent Gnathostoma binucleatum is the only species known to infect humans in Mexico. Although the humoral immune response to G. binucleatum has been analyzed previously, here we evaluated the lymphoproliferative response of peripheral blood lymphocytic cells from patients clinically diagnosed with gnathostomiasis against a crude extract of G. binucleatum using the MTT method, and compared the response to that of healthy subjects. No differences in the response to concanavalin A were found between the groups; however, the response to the crude extract was statistically higher in patients compared to healthy controls (1.188 ± 0.135, and 1.000 ± 0.045, p = 0.0004). Patients at > 6 months after diagnosis showed a higher lymphoproliferative response than the group of healthy subjects, and those at < 6 months after diagnosis (p = 0.0001, and 0.0164 respectively). These data suggest that the parasite induces non-protective immunological memory.

Gnathostoma binucleatum antigens induce peripheral blood lymphocytic cells proliferation

Unidad Académica de Ciencias Químico-Biológicas y Farmacéuticas. Cd de la Cultura Amado Nervo., Universidad Autónoma de Nayarit, CP 63190, Tepic Nayarit, México

Servicio de Dermatología, Secretaría de Salud de Nayarit, Hospital General de Tepic, Av. Enfermería s/n Colonia Centro, CP 63169, Tepic Nayarit, México

Helminthologia

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Gnathostomiasis is an emerging infectious disease, in which the agent Gnathostoma binucleatum is the only species known to infect humans in Mexico. Although...

Gnathostoma binucleatum antigens induce peripheral blood lymphocytic cells proliferation

Published Online: Aug 31, 2013

Page range: 138 - 141

DOI: https://doi.org/10.2478/s11687-013-0122-6

Keywords
Gnathostomiasis, Cellular immune response, G. binucleatum, non-protective immunity

© 2013 Institute of Parasitology, SAS, Košice, Slovakia

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Gnathostoma binucleatum antigens induce peripheral blood lymphocytic cells proliferation

Published Online: Aug 31, 2013

Page range: 138 - 141

DOI: https://doi.org/10.2478/s11687-013-0122-6

KeywordsGnathostomiasis, Cellular immune response, G. binucleatum, non-protective immunity

© 2013 Institute of Parasitology, SAS, Košice, Slovakia

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Keywords
Gnathostomiasis, Cellular immune response, G. binucleatum, non-protective immunity