The species Longidorus profundorum Hooper, 1965 was for the first time recorded in the territory of Slovakia. It was observed in the rhizosphere of Fraxineto-Quercetum forest of riverine plain along Moravia River in south-western part of country, close to boundary with Austria. Morphometrics of females, males and four juvenile stages, morfological and molecular characteristic of Slovakian specimens are presented.