<abstract xml:lang="en" xmlns="http://www.w3.org/1999/xhtml"><p>Uncinaria spp. parasites were collected from two Tsushima leopard cats found on Tsushima Island, Nagasaki prefecture, Japan. One Uncinaria sp. was observed in the large intestines, and the other was observed in the small intestines. The nematode found in the large intestines was determined to be Uncinaria felidis by morphological identification. The other Uncinaria sp. found in the small intestines was morphologically different from U. felidis in the short-length of prevulvar flap. After isolation of genomic DNA of these worms, a second internal transcribed spacer (ITS2) of ribosomal DNA was amplified and sequenced using PCR techniques. The ITS2 region of U. felidis had 222 nucleotide sequences in length. The alignments of ITS2 sequence for Uncinaria felidis and Uncinaria sp. showed one nucleotide (0.45 %) replacement. These differrences may be regarded as intraspecific variation.</p></abstract>

Uncinaria spp. parasites were collected from two Tsushima leopard cats found on Tsushima Island, Nagasaki prefecture, Japan. One Uncinaria sp. was observed in the large intestines, and the other was observed in the small intestines. The nematode found in the large intestines was determined to be Uncinaria felidis by morphological identification. The other Uncinaria sp. found in the small intestines was morphologically different from U. felidis in the short-length of prevulvar flap. After isolation of genomic DNA of these worms, a second internal transcribed spacer (ITS2) of ribosomal DNA was amplified and sequenced using PCR techniques. The ITS2 region of U. felidis had 222 nucleotide sequences in length. The alignments of ITS2 sequence for Uncinaria felidis and Uncinaria sp. showed one nucleotide (0.45 %) replacement. These differrences may be regarded as intraspecific variation.

rDNA ITS sequences of Uncinaria spp. from Tsushima leopard cat (Prionailurus bengalensis euptilura)

Department of Veterinary Pathology, Kagoshima University, 1-21-24 Korimoto, Kagoshima, Japan, 890-0065

Department of Veterinary Microbiology, Faculty of Agriculture, Kagoshima University, 1-21-24 Korimoto, Kagoshima, Japan, 890-0065

Department of Microbiology II, Azabu University, 1-17-71, Fuchinobe, Sagamihara, Kanagawa, Japan, 252-5201

Department of Parasitology, School of Veterinary Medicine, Azabu University, 1-17-71, Fuchinobe, Sagamihara, Kanagawa, Japan, 252-5201

Helminthologia

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Uncinaria spp. parasites were collected from two Tsushima leopard cats found on Tsushima Island, Nagasaki prefecture, Japan. One Uncinaria sp. was observed in...

rDNA ITS sequences of Uncinaria spp. from Tsushima leopard cat (Prionailurus bengalensis euptilura)

Published Online: Aug 03, 2012

Page range: 123 - 127

DOI: https://doi.org/10.2478/s11687-012-0026-x

Keywords
Tsushima leopard, Uncinaria, Genomic DNA, ITS2

© 2012 Institute of Parasitology, SAS, Košice, Slovakia

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

rDNA ITS sequences of Uncinaria spp. from Tsushima leopard cat (Prionailurus bengalensis euptilura)

Published Online: Aug 03, 2012

Page range: 123 - 127

DOI: https://doi.org/10.2478/s11687-012-0026-x

KeywordsTsushima leopard, Uncinaria, Genomic DNA, ITS2

© 2012 Institute of Parasitology, SAS, Košice, Slovakia

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Keywords
Tsushima leopard, Uncinaria, Genomic DNA, ITS2