Molecular confirmation of Xiphinema italiae Meyl, 1953 (Nematoda: Longidoridae) from the Slovak Republic
and | Jun 21, 2009
About this article
Published Online: Jun 21, 2009
Page range: 131 - 134
DOI: https://doi.org/10.2478/s11687-009-0025-8
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© 2009 Institute of Parasitology, SAS, Košice, Slovakia
This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.
Identification of the nematode Xiphinema italiae relies mainly on time-consuming morphological and morphometrical studies. A polymerase chain reaction protocol has been used for the reliable and specific identification of X. italiae. Moreover, four independently evolving molecular markers (cox1- cytochrome c oxidase subunit 1; ITS2-second internal transcribed spacer; 18S gene and D2/D3 expansion segments of 28S gene) were amplified and sequenced in both directions.