<abstract xml:lang="en" xmlns="http://www.w3.org/1999/xhtml"><p>The differences in protein and enzymatic profiles of infective larvae (L3) of Trichostrongylus colubriformis, both induced and non-induced to hypobiosis, have been evaluated by means of SDS-PAGE and densitometric analysis as well as by semiquantitative micromethod API-ZYM (Bio-Mérieux, France). Quantitative differences were identified in protein levels between the induced and non-induced larvae, where the amount of two polypeptides (200–220 kDa) decreased in range 32.3–35.4 % and the amount of six polypeptides (20–28 kDa) increased in range 20.0–27.0 % in the samples of induced larvae. In contrast to non-induced larvae, on gelatin-substrate gel in L3
                in vitro released (IVR) proteases from larvae conditioned to hypobiosis, zones of proteolysis were observed between 21 and 34 kDa.</p></abstract>

The differences in protein and enzymatic profiles of infective larvae (L3) of Trichostrongylus colubriformis, both induced and non-induced to hypobiosis, have been evaluated by means of SDS-PAGE and densitometric analysis as well as by semiquantitative micromethod API-ZYM (Bio-Mérieux, France). Quantitative differences were identified in protein levels between the induced and non-induced larvae, where the amount of two polypeptides (200–220 kDa) decreased in range 32.3–35.4 % and the amount of six polypeptides (20–28 kDa) increased in range 20.0–27.0 % in the samples of induced larvae. In contrast to non-induced larvae, on gelatin-substrate gel in L3
                in vitro released (IVR) proteases from larvae conditioned to hypobiosis, zones of proteolysis were observed between 21 and 34 kDa.

Enzymatic and protein differences between infective larvae of Trichostrongylus colubriformis conditioned or not conditioned to hypobiosis

Department of Zoology and Fisheries, Czech University of Agriculture, Kamýcká 957, 165 21 Prague 6, Suchdol, Czech Republic

Department of Veterinary Sciences, Faculty of Agrobiology, Food and Natural Resources, Czech University of Agriculture, Kamýcká 957, 165 21 Prague 6, Suchdol, Czech Republic

Department of Laboratory Animals Breeding and Hygiene, Institute of Physiology, Academy of Sciences of the Czech Republic, Vídeňská 1083, 142 20, Prague 4, Czech Republic

Helminthologia

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

The differences in protein and enzymatic profiles of infective larvae (L3) of Trichostrongylus colubriformis, both induced and non-induced to hypobiosis, have...

Enzymatic and protein differences between infective larvae of Trichostrongylus colubriformis conditioned or not conditioned to hypobiosis

Published Online: Jun 01, 2006

Page range: 64 - 68

DOI: https://doi.org/10.2478/s11687-006-0013-1

Keywords
Trichostrongylus colubriformis, hypobiosis, protein, enzymes, SDS-PAGE, API-ZYM

© 2006 Institute of Parasitology, SAS, Košice, Slovakia

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Enzymatic and protein differences between infective larvae of Trichostrongylus colubriformis conditioned or not conditioned to hypobiosis

Published Online: Jun 01, 2006

Page range: 64 - 68

DOI: https://doi.org/10.2478/s11687-006-0013-1

KeywordsTrichostrongylus colubriformis, hypobiosis, protein, enzymes, SDS-PAGE, API-ZYM

© 2006 Institute of Parasitology, SAS, Košice, Slovakia

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Keywords
Trichostrongylus colubriformis, hypobiosis, protein, enzymes, SDS-PAGE, API-ZYM