Molecular epidemiology and the clinical impact of carbapenemase-producing Enterobacterales isolates among adult patients: aspects from a Romanian non-teaching hospital

1. Adeolu M, Alnajar S, Naushad S, Gupta RS. Genome-based phylogeny and taxonomy of the ‘Enterobacteriales’: proposal for Enterobacterales ord. nov.divided into the families Enterobacteriaceae, Erwiniaceae fam. nov., Pectobacteriaceae fam. nov., Yersiniaceae fam. nov., Hafniaceae fam. nov., Morganellaceae fam. nov., and Budviciaceae fam. nov. Int J Syst Evol Micr. 2016;66:5575-99. DOI: 10.1099/ijsem.0.00148510.1099/ijsem.0.00148527620848Search in Google Scholar

2. Russo TA, Johnson JR. Diseases caused by Gram-negative enteric bacilli. In: Kasper DL, Fauci AS, Hauser SL, Longo DL, Jameson JL, Loscalzo J, editors. Harrison’s infectious diseases, 3rd edition. USA: McGraw Hill Education 2017;58:507-20.Search in Google Scholar

3. Rodriguez-Bano J, Gutierrez-Gutierrez B, Machuca I, Pascuala A. Treatment of infections caused by extended-spectrum-beta-lactamase-, AmpC-, and carbapenemase-producing Enterobacteriaceae. Clin Microbiol Rev. 2018;31(2):e00079-17. DOI: 10.1128/CMR.00079-1710.1128/CMR.00079-17596768729444952Search in Google Scholar

4. European Centre for Disease Prevention and Control. Rapid Risk Assessment. Carbapenem-resistant Enterobacteriaceae, second update, 26 September 2019. Stockholm: ECDC; 2019. Available from: https://www.ecdc.europa.eu/sites/default/files/documents/carbapenem-resistant-enterobacteriaceae-risk-assessment-rev-2.pdf, accessed 24th May 2020.Search in Google Scholar

5. Magiorakos AP, Srinivasan A, Carey RB, Carmeli Y, Falagas ME, Giske CG et al. Multidrug-resistant, extensively drug-resistant and pandrug-resistant bacteria: an international expert proposal for interim standard definitions for acquired resistance. Clin Microbiol Infect. 2012;18:268-81. DOI: 10.1111/j.1469-0691.2011.03570.x10.1111/j.1469-0691.2011.03570.x21793988Search in Google Scholar

6. The United States Centers for Disease Control and Prevention. Antibiotic resistance threats in the United States 2019 (2019 AR Threats Report), Atlanta: CDC; 2019. Available from: https://www.cdc.gov/drugresistance/pdf/threats-report/2019-ar-threats-report-508.pdf, accessed 24th May 2020.Search in Google Scholar

7. Cui X, Zhang, Du H. Carbapenemases in Enterobacteriaceae: detection and antimicrobial therapy. Front Micro-biol. 2019;10:1823. DOI: 10.3389/fmicb.2019.0182310.3389/fmicb.2019.01823671083731481937Search in Google Scholar

8. Morrill HJ, Pogue JM, Kaye KS, LaPlante KL. Treatment options for carbapenem-resistant Enterobacteriaceae infections. Open Forum Infect Dis. 2015;2(2):ofv050. DOI: 10.1093/ofid/ofv05010.1093/ofid/ofv050446259326125030Search in Google Scholar

9. Bonomo RA, Burd EM, Conly J, Limbago BM, Poirel L, Segre JA et al. Carbapenemase-producing organisms: a global scourge. Clin Infect Dis. 2018;66:1290-7. DOI: 10.1093/cid/cix89310.1093/cid/cix893588473929165604Search in Google Scholar

10. van Duin D, Doi Y. The global epidemiology of carbapenemase-producing Enterobacte-riaceae. Virulence 2017;8(4):460-9. DOI: 10.1080/21505594.2016.122234310.1080/21505594.2016.1222343547770527593176Search in Google Scholar

11. European Centre for Disease Prevention and Control. Rapid Risk Assessment. Emergence of resistance to ceftazidime-avibactam in carbapenem-resistant Enterobacteriaceae, 12 June 2018. Stockholm: ECDC; 2018. Available from:https://www.ecdc.europa.eu/sites/default/files/documents/RRA-Emergence-of-resistance-to%20CAZ-AVI-in-CRE-Enterobacteriaceae.pdf, accessed 24th May 2020.Search in Google Scholar

12. Szekely E, Damjanova I, Janvari L, Vas KE, Molnar S, Bilca DV, et al. First description of blaNDM-1, blaOXA-48, blaOXA-181 producing Enterobacteriaceae strains in Romania. Int J of Med Microbiol. 2013;303(8):697-700. DOI: 10.1016/j. ijmm.2013.10.001Search in Google Scholar

13. Braun SD, Dorneanu OS, Vremera T, Reissig A, Monecke S, Ehricht R. Carbapenemase-producing Enterobacteriaceae: a 2-year surveillance in a hospital in Iaşi, Romania. Future Microbiol. 2016;11(3):391-401. DOI: 10.2217/fmb.15.14810.2217/fmb.15.14826974389Search in Google Scholar

14. Lixandru BE, Cotar AI, Straut M, Usein CR, Cristea D, Ciontea S et al. Carbapenemase-producing Klebsiella pneumoniae in Romania: A Six-Month Survey. PLoS One. 2015;10:e0143214. DOI: 10.1371/journal. pone.0143214Search in Google Scholar

15. Gheorghe I, Czobor I, Chifiriuc MC, Borcan E, Ghita C, Banu O et al. Molecular screening of carbapenemase-producing Gram-negative strains in Romanian intensive care units during a one year survey. J Med Microbiol. 2014;63:1303-10. DOI: 10.1099/jmm.0.074039-010.1099/jmm.0.074039-025060972Search in Google Scholar

16. Rafila A, Talapan D, Dorobat OM, Popescu GA, Pitigoi D, Florea D et al. Emergence of carbapenemase-producing Enterobacteriaceae, a public health threat: a Romanian Infectious Disease Hospital based study. Rev Romana Med Lab. 2015;23:295-301. DOI: 10.1515/rrlm-2015-002410.1515/rrlm-2015-0024Search in Google Scholar

17. Foldes A, Bilca DV, Szekely E. Phenotypic and molecular identification of carbapenemase-producing Enterobacteriaceae - challenges in diagnosis and treatment. Rev Romana Med Lab. 2018;26:221-30. DOI: 10.2478/rrlm-2018-001810.2478/rrlm-2018-0018Search in Google Scholar

18. Molnar S, Flonta MMM, Almas A, Buzea M, Licker M, Rus M et al. Dissemination of NDM-1 carbapenemase-producer Providencia stuartii strains in Romanian hospitals: a multicentre study. J Hosp Infect. 2019;103:165-9. DOI: 10.1016/j.jhin.2019.04.01510.1016/j.jhin.2019.04.015Search in Google Scholar

19. Dortet L, Flonta M, Boudehen YM, Creton E, Bernabeu S, Vogel A et al. Dissemination of carbapenemase producing Enterobacteriaceae and Pseudomonas aeruginosa in Romania. Antimicrob Agents Chemother 2015;59(11):7100-3. DOI: 10.1128/AAC.01512-1510.1128/AAC.01512-15Search in Google Scholar

20. Popescu C, Popescu GA, Dorobat O, Rafila A, Tanase D, Mikula C, et al. OXA-48-carbapenemase-producing Klebsiella pneumoniae infections - the first cases diagnosed in Romanian National Institute of Infectious Diseases. Rev Romana Med Lab. 2017;25(1):55-61. DOI: 10.1515/rrlm-2017-000610.1515/rrlm-2017-0006Search in Google Scholar

21. Predoiu G, Muntean MM, Muntean A, Popa MI, Sandu IT, Aurelian J et al. Phenotypic and molecular detection of carbapenemase-producing gram negative bacilli in a tertiary urology hospital. EurU-rolSuppl. 2018;17(12):e2736. DOI: 10.1016/S1569-9056(18)33573-510.1016/S1569-9056(18)33573-5Search in Google Scholar

22. Performance Standards for antimicrobial susceptibility testing, 27th informational supplement. CLSI document M100-S27. Clinical and Laboratory Standards Institute, 2017.Search in Google Scholar

23. van der Zwaluw K, de Haan A, Pluister GN, Boots-ma HJ, de Neeling AJ, Schouls LM - The carbapenem inactivation method (CIM), a simple and low-cost alternative for the Carba NP Test to assess phenotypic carbapenemase activity in Gram-negative rods. PLoS ONE. 2015;10(3):1-13. e0123690. DOI: 10.1371/journal.pone.012369010.1371/journal.pone.0123690437085225798828Search in Google Scholar

24. Standard Operating Procedure for PulseNet PFGE of Escherichia coli O157:H7, Escherichia coli non-O157 (STEC), Salmonella serotypes, Shigella sonnei and Shigella flexneri. Available from: https://www.cdc.gov/pulsenet/pdf/ecoli-shigella-salmonella-pfge-protocol-508c.pdf, accessed 25th May 2020.Search in Google Scholar

25. Tenover FC, Arbeit RD, Goering RV, Mickelsen PA, Murray BE, Persing DH et al. Interpreting chromosomal DNA restriction patterns produced by Pulsed-Field Gel Electrophoresis: criteria for bacterial strain typing. J Clin Microbiol. 1995:33(9):2233-9. DOI: 10.1128/JCM.33.9.2233-2239.199510.1128/jcm.33.9.2233-2239.19952283857494007Search in Google Scholar

26. World Health Organization. Global antimicrobial resistance surveillance system (GLASS) report: early implementation 2016-2017. Geneva: WHO; 2017. Available from:https://apps.who.int/iris/bitstream/handle/10665/259744/9789241513449-eng.pdf;jsessionid=F157BC737B4E5899BE88D4F414129581?sequence=1, accessed 26th May 2020.Search in Google Scholar

27. European Centre for Disease Prevention and Control. Surveillance Report. Surveillance of antimicrobial resistance in Europe 2018. Stockholm: ECDC; 2019. Available from: https://www.ecdc.europa.eu/sites/default/files/documents/surveillance-antimicrobial-resistance-Europe-2018.pdf, accessed 26th May 2020.Search in Google Scholar

28. European Centre for Disease Prevention and Control. Outbreak of carbapenemase-producing (NDM-1 and OXA-48) and colistin-resistant Klebsiellapneumoniae ST307, north-east Germany, 2019. 28 October 2019. Stockholm: ECDC; 2019. Available from: https://www.ecdc.europa.eu/sites/default/files/documents/Klebsiella-pneumoniae-resistance-Germany-risk-assessment.pdf, accessed 29th May 2020.Search in Google Scholar

29. Durante-Mangoni E, Andini R, Zampino R. Management of carbapenem-resistant Enterobacteriaceae infections. Clin Microbiol. Infect 2019;25:943-50. DOI: 10.1016/j.cmi.2019.04.01310.1016/j.cmi.2019.04.01331004767Search in Google Scholar

30. Jacobs DM, Courtney Safir M, Huang D, Minhaj F, Parker A, Rao GG. Triple combination antibiotic therapy for carbapenemase-producing Klebsiella pneumoniae: a systematic review. Ann Clin Microbiol Antimicrob. 2017;16(1):76. DOI: 10.1186/s12941-017-0249-210.1186/s12941-017-0249-2570208929178957Search in Google Scholar

31. Du X, He F, Shi Q, Zhao F, Xu J, Fu Y et al. The rapid emergence of tigecycline resistance in blaKPC2 harboring Klebsiella pneumoniae, as mediated in vivo by mutation in tetA during tigecycline treatment. Front Microbiol. 2018;9:648. DOI: 10.3389/fmicb.2018.0064810.3389/fmicb.2018.00648589564929675006Search in Google Scholar

32. Jayol A, Nordmann P, Lehours P, Poirel L, Dubois V. Comparison of methods for detection of plasmid-mediated and chromosomally encoded colistin resistance in Enterobacteriaceae. Clin Microbiol Infect. 2018;24:175-9. DOI: 10.1016/j.cmi.2017.06.00210.1016/j.cmi.2017.06.00228606644Search in Google Scholar

33. Cardoso T, Almeida M, Friedman ND, Aragao I, Costa-Pereira A, Sarmento AE et al. Classification of healthcare-associated infection: a systematic review 10 years after the first proposal. BMC Med. 2014;12:40. DOI: 10.1186/1741-7015-12-4010.1186/1741-7015-12-40401661224597462Search in Google Scholar

34. Kohler PP, Melano RG, Patel SN, Shafinaz S, Faheem A, Coleman BL et al. Emergence of carbapenemase-producing Enterobacteriaceae, South-Central Ontario, Canada. Emerg Infect Dis. 2018;24(9):1674-82. DOI: 10.3201/eid2409.18016410.3201/eid2409.180164610640730124197Search in Google Scholar

35. Safdar N, Maki DG. The commonality of risk factors for nosocomial colonization and infection with antimicrobial-resistant Staphylococcus aureus, Enterococcus, Gram-negative bacilli, Clostridium difficile, and Candida. Ann Intern Med. 2002;136:834-44. DOI: 10.7326/0003-4819-136-11-200206040-0001310.7326/0003-4819-136-11-200206040-0001312044132Search in Google Scholar

36. Alexander EL, Loutit J, Tumbarello M, Wunderink R, Felton T, Daikos G et al. Carbapenem-resistant Enterobacteriaceae infections: results from a retrospective series and implications for the design of prospective clinical trials. Open Forum Infect Dis. 2017;4(2):ofx063. DOI: 10.1093/ofid/ofx06310.1093/ofid/ofx063545166428584849Search in Google Scholar

37. Martin A, Fahrbach K, Zhao Q, Lodise T. Association between carbapenem resistance and mortality among adult, hospitalized patients with serious infections due to Enterobacteriaceae: results of a systematic literature review and meta-analysis. Open Forum Infect Dis. 2018;5(7):ofy150. DOI: 10.1093/ofid/ofy15010.1093/ofid/ofy150605422830046639Search in Google Scholar

38. Tischendorf J, Almeida de Avila R, Safdar N. Risk of infection following colonization with carbapenem-resistant Enterobactericeae: A systematic review. Am J Infect Control. 2016;44(5):539-43. DOI: 10.1016/j. ajic.2015.12.005Search in Google Scholar