<abstract xmlns="http://www.w3.org/1999/xhtml"><p><bold>Aim:</bold> The aim of the study is to investigate the effect of Cynomorium songaricum (CS) on the damage caused by cyclophosphamide (CP) in SD rats. <bold>Methods:</bold> Rats with CP-induced oligoasthenospermia were treated with different concentration of CS. Testicle weight, epididymal sperm count (ESC), sperm motility, and serum testosterone were analyzed, and expression levels of Thy1, Oct4, PLZF, C-kit, and GDNF were detected in testis tissues. Transmission electron microscopy (TEM) was performed to observe the effect of CS on the spermatogenic damage by CP. <bold>Results:</bold> Compared with the CP group, there were significant differences in testicle weight, ESC, and sperm motility (p&lt;0.05) observed in all concentrations of CS and CP+VitE groups (p&lt;0.05). There were no significant differences in serum testosterone among the 6 groups (p>0.05). The qPCR results revealed a significant difference in Thy1, Oct4, PLZF and GDNF expression between the CP group and CS group (p &lt;0.05), but there was no significant difference in C-kit between the two groups (p>0.05). The damage of CP was cured by CS observed under TEM. <bold>Conclusion:</bold> CS can increase sperm counts in the epididymis and improve sperm motility and has a therapeutic effect on the spermatogenic damage caused by CP in SD rats.</p></abstract>

Aim: The aim of the study is to investigate the effect of Cynomorium songaricum (CS) on the damage caused by cyclophosphamide (CP) in SD rats. Methods: Rats with CP-induced oligoasthenospermia were treated with different concentration of CS. Testicle weight, epididymal sperm count (ESC), sperm motility, and serum testosterone were analyzed, and expression levels of Thy1, Oct4, PLZF, C-kit, and GDNF were detected in testis tissues. Transmission electron microscopy (TEM) was performed to observe the effect of CS on the spermatogenic damage by CP. Results: Compared with the CP group, there were significant differences in testicle weight, ESC, and sperm motility (p<0.05) observed in all concentrations of CS and CP+VitE groups (p<0.05). There were no significant differences in serum testosterone among the 6 groups (p>0.05). The qPCR results revealed a significant difference in Thy1, Oct4, PLZF and GDNF expression between the CP group and CS group (p <0.05), but there was no significant difference in C-kit between the two groups (p>0.05). The damage of CP was cured by CS observed under TEM. Conclusion: CS can increase sperm counts in the epididymis and improve sperm motility and has a therapeutic effect on the spermatogenic damage caused by CP in SD rats.

Cynomorium Songaricum may protect against spermatogenic damage caused by cyclophosphamide in SD rats

The third affiliated hospital of Soochow University

Revista Romana de Medicina de Laborator

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Aim: The aim of the study is to investigate the effect of Cynomorium songaricum (CS) on the damage caused by cyclophosphamide (CP) in SD rats. Methods: Rats with CP-induced oligoasthenospermia were treated with different concentration of CS. Testicle weight, epididymal sperm count (ESC), sperm motility, and serum testosterone were analyzed, and expression levels of Thy1, Oct4, PLZF, C-kit, and GDNF were detected in testis tissues. Transmission electron microscopy (TEM) was performed to observe the effect of CS on the spermatogenic damage by CP. Results: Compared with the CP group, there were significant differences in testicle weight, ESC, and sperm motility (p0.05). The qPCR results revealed a significant difference in Thy1, Oct4, PLZF and GDNF expression between the CP group and CS group (p 0.05). The damage of CP was cured by CS observed under TEM. Conclusion: CS can increase sperm counts in the epididymis and improve sperm motility and has a therapeutic effect on the spermatogenic damage caused by CP in SD rats.

Aim: The aim of the study is to investigate the effect of Cynomorium songaricum (CS) on the damage caused by cyclophosphamide (CP) in SD rats. Methods: Rats...

Cynomorium Songaricum may protect against spermatogenic damage caused by cyclophosphamide in SD rats

Published Online: Jul 30, 2019

Page range: 291 - 303

Received: Dec 19, 2018

Accepted: Jul 11, 2019

DOI: https://doi.org/10.2478/rrlm-2019-0031

Keywords
Cynomorium Songaricum, spermatogenic damage, cyclophosphamide, epididymal sperm count, sperm motility

© 2019 Xiaoxiao Han et al., published by Sciendo

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Cynomorium Songaricum may protect against spermatogenic damage caused by cyclophosphamide in SD rats

Published Online: Jul 30, 2019

Page range: 291 - 303

Received: Dec 19, 2018

Accepted: Jul 11, 2019

DOI: https://doi.org/10.2478/rrlm-2019-0031

KeywordsCynomorium Songaricum, spermatogenic damage, cyclophosphamide, epididymal sperm count, sperm motility

© 2019 Xiaoxiao Han et al., published by Sciendo

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Keywords
Cynomorium Songaricum, spermatogenic damage, cyclophosphamide, epididymal sperm count, sperm motility