Evaluation of the training program for p16/ Ki-67 dual immunocytochemical staining interpretation for laboratory staff without experience in cervical cytology and immunocytochemistry
, , , , , , and | Mar 26, 2020
About this article
Article Category: Research Article
Published Online: Mar 26, 2020
Page range: 201 - 208
Received: Jan 22, 2020
Accepted: Mar 04, 2020
DOI: https://doi.org/10.2478/raon-2020-0018
© 2020 Veronika Kloboves Prevodnik, Ziva Pohar Marinsek, Janja Zalar, Hermina Rozina, Nika Kotnik, Tine Jerman, Jerneja Varl, Urska Ivanus, published by Sciendo
This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.
Figure 1
Figure 2
Figure 3
Figure 4
Individual comparison of p16/Ki-67 agreement and performance between students, senior technologist and reference
| Training | Reviewers | OPA | McNemar’s test p | κ (cohen) (95% CI) |
|---|---|---|---|---|
| S1/Reference | 81.4% | 0.000 | 0.512 (CI: 0.329–0,696) | |
| S2/Reference | 81.4% | 0.006 | 0.527 (CI: 0.349–0,705) | |
| Initial training | SC (S1)/ Reference | 94.1% | 0.131 | 0.859 (CI: 0.758–0.960) |
| (N = 118) | SC (S2)/ Reference | 94.1% | 0.131 | 0.859 (CI: 0.758–0.960) |
| SC (S1)/SC (S2) | 100.0% | / | 1.000 (CI: /) | |
| S1/Reference | 78.3% | 0.000 | 0.556 (Cl: 0.472–0.641) | |
| S2/Reference | 87.2% | 0.775 | 0.713 (Cl: 0.638–0.788) | |
| Addtional (N = 383) | SC (S1)/Reference | 86.2% | 0.006 | 0.700 (Cl: 0.625–0,775) |
| SC (S2)/Reference | 86.4% | 0.004 | 0.707 (Cl: 0.632–0.781) | |
| SC (S1)/SC (S2) | 98.7% | 1.000 | 0.973 (CI: 0.949–0.996) |
p16/Ki-67 study results and CIN2+ outcome for students, senior cytotechnologist and reference
| Reviewer | Categories of p16/Ki67 dual staining result | Initial training (N = 118) | Additional training (N = 383) | ||
|---|---|---|---|---|---|
| p16/Ki67 dual staining result N, (%) | CIN2+ outcomes N (PV, %)* | p16/Ki67 dual staining result (N, %) | CIN2+ outcomes N (PV, %)* | ||
| positive | 91 (77.1) | 65 (71.4) | 171 (44.6) | 65 (38.0) | |
| S1 | suspicious | 7 (5.9) | 2 (28.6) | 7 (1.8) | 2 (28.6) |
| negative | 20 (16.9) | 2 (10.0) | 205 (53.5) | 10 (4.9) | |
| unsatisfactory | 0 (0.0) | 0 (0.0) | 0 (0.0) | 0 (0.0) | |
| positive | 92 (78.0) | 66 (71.7) | 129 (33.7) | 64 (49.6) | |
| S2 | suspicious | 2 (1.7) | 0 (0.0) | 1 (0.3) | 1 (100.0) |
| negative | 24 (20.3) | 3 (12.5) | 253 (66.1) | 12 (4.7) | |
| unsatisfactory | 0 (0) | 0 (0.0) | 0 (0.0) | 0 (0.0) | |
| positive | 83 (70.3) | 65 (78.3) | 143 (37.9) | 65 (45.5) | |
| suspicious | 2 (1.7) | 2 (100.0) | 5 (1.3) | 0 (0.0) | |
| SC (S1) | negative | 32 (27.1) | 2 (6.3) | 235 (61.4) | 12 (5.1) |
| unsatisfactory | 1 (0.8) | 0 (0.0) | 0 (0.0) | 0 (0.0) | |
| positive | 83 (70.3) | 65 (78.3) | 145 (37.9) | 64 (44.1) | |
| suspicious | 2 (1.7) | 2 (100.0) | 4 (1.0) | 0 (0.0) | |
| SC (S2) | negative | 32 (27.1) | 2 (6.3) | 234 (61.1) | 13 (5.6) |
| unsatisfactory | 1 (0.8) | 0 (0.0) | 0 (0.0) | 0 (0.0) | |
| positive | 78 (66.1) | 64 (82.1) | 114 (29.8) | 64 (56.1) | |
| Reference | suspicious | 2 (1.7) | 0 (0) | 13 (3.4) | 3 (23.1) |
| negative | 38 (32.2) | 5 (13.2) | 255 (66.6) | 10 (3.9) | |
| unsatisfactory | 0 (0.0) | 0 (0.0) | 1 (0.3) | 0 (0.0) | |