<abstract xmlns="http://www.w3.org/1999/xhtml"><p>Flaky graphene oxide was activated with divinylsulfone followed by immobilization of the β-galactosidase enzyme. An active and stable preparation was obtained. β-galactosidase stability after immobilization was much higher than with the native enzyme. The half-life time of the immobilized enzyme was estimated as 165 hours, while for the native form, the estimate was only 5 hours. The developed procedure for the preparation of flaked graphene and its use in the chemical immobilization of enzymes can be used for any enzyme. A processing solution for continuous operation was proposed and verified using cow’s milk, with lactose as the hydrolysed substrate, as a dosing stream. Lactose, a milk sugar, was effectively hydrolysed. Product for allergy sufferers who cannot digest lactose has been obtained in this way.</p></abstract>

Flaky graphene oxide was activated with divinylsulfone followed by immobilization of the β-galactosidase enzyme. An active and stable preparation was obtained. β-galactosidase stability after immobilization was much higher than with the native enzyme. The half-life time of the immobilized enzyme was estimated as 165 hours, while for the native form, the estimate was only 5 hours. The developed procedure for the preparation of flaked graphene and its use in the chemical immobilization of enzymes can be used for any enzyme. A processing solution for continuous operation was proposed and verified using cow’s milk, with lactose as the hydrolysed substrate, as a dosing stream. Lactose, a milk sugar, was effectively hydrolysed. Product for allergy sufferers who cannot digest lactose has been obtained in this way.

Graphene oxide flake activation via divinylsulfone – a procedure for efficient β-galactosidase immobilization

Wroclaw University of Science and Technology

Department of Bioprocess and Biomedical Engineering

Polish Journal of Chemical Technology

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Flaky graphene oxide was activated with divinylsulfone followed by immobilization of the β-galactosidase enzyme. An active and stable preparation was obtained....

Graphene oxide flake activation via divinylsulfone – a procedure for efficient β-galactosidase immobilization

Published Online: Mar 16, 2019

Page range: 27 - 32

DOI: https://doi.org/10.2478/pjct-2019-0006

Keywords
graphene oxide, enzyme chemical immobilization, divinylsulfone, β-galactosidase, lactose-free product

© 2019 Anna Trusek, published by Sciendo

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Graphene oxide flake activation via divinylsulfone – a procedure for efficient β-galactosidase immobilization

Published Online: Mar 16, 2019

Page range: 27 - 32

DOI: https://doi.org/10.2478/pjct-2019-0006

Keywordsgraphene oxide, enzyme chemical immobilization, divinylsulfone, β-galactosidase, lactose-free product

© 2019 Anna Trusek, published by Sciendo

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Keywords
graphene oxide, enzyme chemical immobilization, divinylsulfone, β-galactosidase, lactose-free product