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The Influence of Spent Mushroom Substrate Fertilization on The Selected Properties of Arable Soil


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Selected properties of soil humus horizon after the first and second year of the experiment

Experimental objectspHKClHhCECVCorgN-totC:N
mmol(+)·kg−1%g·kg−1
After first year of cultivation
Control4.7920.667.676.66.020.619.84
SMS5.1122.590.380.37.650.799.68
After second year of cultivation
Control4.6124.064.572,96.090.5810.5
SMS4.6924.479.276,47.650.6312.1

The nitrogen in separate fractions by acid hydrolysis in the A horizon of the field experiment objects

Experimental objectsNOSNOEHNODHNONH
g·kg−1% N-totg·kg−1% N-totg·kg−1% N-totg·kg−1% N-tot
First year of cultivation
Control1.332.1812.019.719.632.128.146.1
SMS1.682.1314.518.425.432.237.447.3
Second year of cultivation
Control1.202.0711.519.816.828.928.549.1
SMS1.822.9012.019.020.532.528.244.8

Selected properties of spent mushroom substrate used in the experiment

Dry matter (DM)CorgN-totC:NpHH20
g·kg−1
30927823.311.97.25

Nitrogen forms and the methods of their recovery [Kalembasa, 1995; Becher, Pakuła, 2014]

Nitrogen form (operational)Method of recovery
N-NH4 – ammonium formdistillation from 0.25 M KCl extract after alkalization with MgO
N-NOx – nitrate forms (III and V): N-NO3 and N-NO2distillation from 0.25 M KCl extract after alkalization with N-NH4 and application of Devards reductive mixture
NMIN – nitrogen in mineral compoundsNMIN = N-NH4 +N-NOx
NORG – nitrogen in organic compoundsNORG =N-tot. – NMIN
NKClmeasured in 0.25 M KCl extract after mineralization of the solution
NOS – soluble organic nitrogenNOS =NKCl – NMIN
NOEH – easily hydrolysing organic nitrogenmeasured after hydrolysis in 0.25 M H2SO4 and mineralization of the solution
NODH – organic nitrogen difficult to hydrolysemeasured after hydrolysis in 2.5 M H2SO4 and mineralization of the solution
NONH – not hydrolysing organic nitrogenmeasured after post-extraction and mineralization of the residues concentrated H2SO4

Mineral forms of nitrogen in separate fractions from the A horizon of the field experimental objects

Experimental objectsN-NH4N-NO3 and N-NO2NMIN
g·kg−1% N-totg·kg−1% N-totg·kg−1% N-tot
First year of cultivation
Control1.682.751.191.952.874.70
SMS1.752.221.051.332.803.55
Second year of cultivation
Control1.542.650.981.692.524.34
SMS1.963.131.101.583.064.68

The coefficients of correlation between the nitrogen content in different fractions and selected properties of A horizon of the investigated soils

ParameterN-NH4N-NOxNOSNOEHNODHNONH
N-tot0.350.130.68*0.82*0.80*0.14
Corg0.500.200.78*0.390.540.13
C:N0.260.100.24−0.44−0.21−0.03
pHKCl−0.23−0.480.210.330.57−0.51
Hh0.360.310.310.20−0.25−0.71*
CEC0.58*0.200.67*0.72*0.70*−0.37
V0.25−0.020.380.430.73*−0.79*

The mineral nitrogen forms in the investigated spent mushroom substrate

N-NH4N-NO3 and N-NO2NMIN
g·kg−1% N-totg·kg−1% N-totg·kg−1% N-tot
0.8003.430.0950.400.8953.84

The nitrogen organic compounds in separate fractions by acid hydrolysis in the investigated spent mushroom substrate

NOSNOEHNODHNONH
g·kg−1% N-totg·kg−1% N-totg·kg−1% N-totg·kg−1% N-tot
2.8112.04.2918.44.1517.811.547.8
eISSN:
2353-8589
Language:
English
Publication timeframe:
4 times per year
Journal Subjects:
Life Sciences, Ecology