<abstract xmlns="http://www.w3.org/1999/xhtml"><p>Ergosan contains 1% alginic acid extracted from two brown sea weeds. Little is known about the target organs and anatomical distribution of Ergosan (alginic acid) in fish. Therefore, feasibility of developing alginic acid nanoparticles to detect target organ in rainbow trout is interesting. To make nanoparticles, Ergosan extract (alginic acid) was irradiated at 30 kGy in a cobalt-60 irradiator and characterized by transmission electron microscopy (TEM) and Fourier transform infrared spectroscopy (FTIR). Results from TEM images showed that particle sizes of irradiated alginic acid ranged from 30 to 70 nm. The FTIR results indicated that gamma irradiation had no significant influence on the basic structure of alginic acid. Later, alginic acid nanoparticles were successively labelled with 67Ga-gallium chloride. The biodistribution of irradiated Ergosan in normal rainbow trout showed highest uptake in intestine and kidney and then in liver and kidney at 4- and 24-h post injection, respectively. Single-photon emission computed tomography (SPECT) images also demonstrated target specific binding of the tracer at 4- and 24-h post injection. In conclusion, the feed supplemented with alginic acid nanoparticles enhanced SPECT images of gastrointestinal morphology and immunity system in normal rainbow trout.</p></abstract>

Ergosan contains 1% alginic acid extracted from two brown sea weeds. Little is known about the target organs and anatomical distribution of Ergosan (alginic acid) in fish. Therefore, feasibility of developing alginic acid nanoparticles to detect target organ in rainbow trout is interesting. To make nanoparticles, Ergosan extract (alginic acid) was irradiated at 30 kGy in a cobalt-60 irradiator and characterized by transmission electron microscopy (TEM) and Fourier transform infrared spectroscopy (FTIR). Results from TEM images showed that particle sizes of irradiated alginic acid ranged from 30 to 70 nm. The FTIR results indicated that gamma irradiation had no significant influence on the basic structure of alginic acid. Later, alginic acid nanoparticles were successively labelled with 67Ga-gallium chloride. The biodistribution of irradiated Ergosan in normal rainbow trout showed highest uptake in intestine and kidney and then in liver and kidney at 4- and 24-h post injection, respectively. Single-photon emission computed tomography (SPECT) images also demonstrated target specific binding of the tracer at 4- and 24-h post injection. In conclusion, the feed supplemented with alginic acid nanoparticles enhanced SPECT images of gastrointestinal morphology and immunity system in normal rainbow trout.

Preparation and anatomical distribution study of <sup>67</sup>Ga-alginic acid nanoparticles for SPECT purposes in rainbow trout (Oncorhynchus mykiss)

Nuclear Science and Technology Research Institute, Karaj, Iran, Tel.: +9826 3441 1101,

Department of Fisheries and Environment Science, Faculty of Natural Resources, University of Tehran, Tehran, Iran

Animal Production and Health Laboratory, International Atomic Energy Agency (IAEA), Vienna, Austria

Nuclear Science and Technology Research Institute, Karaj, Iran, Tel.: +9826 3441 1101

Department of Food Hygiene and Aquatic Animals, Faculty of Veterinary Medicine, University of Tabriz, Tabriz, Iran

Islamic Azad University, Branch of Kazeroon, Kazeroon, Iran

Nukleonika

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Preparation and anatomical distribution study of Ga-alginic acid nanoparticles for SPECT purposes in rainbow trout (Oncorhynchus mykiss)

Ergosan contains 1% alginic acid extracted from two brown sea weeds. Little is known about the target organs and anatomical distribution of Ergosan (alginic...

Preparation and anatomical distribution study of ⁶⁷Ga-alginic acid nanoparticles for SPECT purposes in rainbow trout (Oncorhynchus mykiss)

Published Online: Dec 30, 2014

Page range: 153 - 159

Received: Jan 08, 2014

Accepted: Sep 08, 2014

DOI: https://doi.org/10.2478/nuka-2014-0019

© by Marzieh Heidarieh

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Preparation and anatomical distribution study of 67Ga-alginic acid nanoparticles for SPECT purposes in rainbow trout (Oncorhynchus mykiss)

Published Online: Dec 30, 2014

Page range: 153 - 159

Received: Jan 08, 2014

Accepted: Sep 08, 2014

DOI: https://doi.org/10.2478/nuka-2014-0019

© by Marzieh Heidarieh

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Preparation and anatomical distribution study of ⁶⁷Ga-alginic acid nanoparticles for SPECT purposes in rainbow trout (Oncorhynchus mykiss)