<abstract xmlns="http://www.w3.org/1999/xhtml">This study provides insight into the development and validation of the simple, rapid and sensitive method of Liquid Chromatography-Tandem Mass Spectrometry for determining the residues of dexamethasone in bovine milk. The maximum residue limit for dexamethasone in bovine milk is 0.3 ng/ml−1. Chromatographic separation was achieved on an Agilent Proshell 120 SB:C18 (2.7µm 100x3.0 mm) column. The bovine milk was extracted with ethyl acetate, and the evaporated elution was dissolved with hexane, water and methanol, and analyzed by the LC-MS/MS method. The mobile phase A was a solution of 5 Mm ammonium formate in water, while the mobile phase B was 0.1% formic acid in acetonitrile. The calibration graphics were prepared within the range of 0.15–0.6 ng/ml−1, and a successful linearity was achieved (r ≥0.999). The limit of detection was 0.016 ng/ml−1. The decision limit (CCα) and detection capability (CCβ) were 0.34 and 0.38 ng/ml−1, respectively.</abstract>

This study provides insight into the development and validation of the simple, rapid and sensitive method of Liquid Chromatography-Tandem Mass Spectrometry for determining the residues of dexamethasone in bovine milk. The maximum residue limit for dexamethasone in bovine milk is 0.3 ng/ml−1. Chromatographic separation was achieved on an Agilent Proshell 120 SB:C18 (2.7µm 100x3.0 mm) column. The bovine milk was extracted with ethyl acetate, and the evaporated elution was dissolved with hexane, water and methanol, and analyzed by the LC-MS/MS method. The mobile phase A was a solution of 5 Mm ammonium formate in water, while the mobile phase B was 0.1% formic acid in acetonitrile. The calibration graphics were prepared within the range of 0.15–0.6 ng/ml−1, and a successful linearity was achieved (r ≥0.999). The limit of detection was 0.016 ng/ml−1. The decision limit (CCα) and detection capability (CCβ) were 0.34 and 0.38 ng/ml−1, respectively.

A Simple and Rapid LC-MS/MS Method for Determination of Dexamethasone in Bovine Milk

Department of Pharmacology, Pendik Veterinary Control Institute

Macedonian Veterinary Review

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 License.

This study provides insight into the development and validation of the simple, rapid and sensitive method of Liquid Chromatography-Tandem Mass Spectrometry for...

A Simple and Rapid LC-MS/MS Method for Determination of Dexamethasone in Bovine Milk

Published Online: Mar 15, 2020

Page range: 69 - 73

Received: Sep 25, 2019

Accepted: Jan 27, 2020

DOI: https://doi.org/10.2478/macvetrev-2020-0014

Keywords
dexamethasone, LC-MS/MS, milk

© 2020 Mehmet Nihat Ural et al., published by Sciendo

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 License.

A Simple and Rapid LC-MS/MS Method for Determination of Dexamethasone in Bovine Milk

Published Online: Mar 15, 2020

Page range: 69 - 73

Received: Sep 25, 2019

Accepted: Jan 27, 2020

DOI: https://doi.org/10.2478/macvetrev-2020-0014

Keywordsdexamethasone, LC-MS/MS, milk

© 2020 Mehmet Nihat Ural et al., published by Sciendo

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 License.

Keywords
dexamethasone, LC-MS/MS, milk