<abstract xmlns="http://www.w3.org/1999/xhtml"><p>Reduced glutathione (GSH) and homologous ram seminal plasma (HSP), used as additives in cryopreserving (CP) media prior to freezing, showed conflicting results in retaining structural integrity and progressive motility in post-thawed ram spermatozoa. The aims of this research were: (1) to assess the effect of GSH and/or HSP supplementation via soybean-lecithin CP extender on cryopreserved ram spermatozoa viability, morphology and motility pattern; and (2) to assess the effect of incubation in the context of the previous aim. Quantitatively and qualitatively, homogenized and pooled ram ejaculates (N=10) were extended with one of the following extenders: control (C) – tris-based, GSH and HSP-free, experimental-1 (E1) – C + GSH 5 mM, experimental-2 (E2) – C + HSP 20 % and experimental-3 (E3) - GSH 5 mM + HSP 20 %. Following thawing, samples were taken at 0- and 3-hours from each group (n=10) and were assessed for spermatozoa viability, morphology, and motility pattern. C-0h samples yielded a spermatozoa population with low viability, altered head morphology and highly deviated motility pattern. E3-3h samples yielded spermatozoa with unaffected viability, head morphology and high progressive motility. In conclusion, E3 extender added to cryopreserved-thawed ram spermatozoa is most efficient in obtaining high viability, unaltered head morphology, and progressive motility.</p></abstract>

Reduced glutathione (GSH) and homologous ram seminal plasma (HSP), used as additives in cryopreserving (CP) media prior to freezing, showed conflicting results in retaining structural integrity and progressive motility in post-thawed ram spermatozoa. The aims of this research were: (1) to assess the effect of GSH and/or HSP supplementation via soybean-lecithin CP extender on cryopreserved ram spermatozoa viability, morphology and motility pattern; and (2) to assess the effect of incubation in the context of the previous aim. Quantitatively and qualitatively, homogenized and pooled ram ejaculates (N=10) were extended with one of the following extenders: control (C) – tris-based, GSH and HSP-free, experimental-1 (E1) – C + GSH 5 mM, experimental-2 (E2) – C + HSP 20 % and experimental-3 (E3) - GSH 5 mM + HSP 20 %. Following thawing, samples were taken at 0- and 3-hours from each group (n=10) and were assessed for spermatozoa viability, morphology, and motility pattern. C-0h samples yielded a spermatozoa population with low viability, altered head morphology and highly deviated motility pattern. E3-3h samples yielded spermatozoa with unaffected viability, head morphology and high progressive motility. In conclusion, E3 extender added to cryopreserved-thawed ram spermatozoa is most efficient in obtaining high viability, unaltered head morphology, and progressive motility.

Homologous Seminal Plasma and Glutathione Promote Pre-Capacitation Motility and Structural Stability of Cryopreserved Ram Spermatozoa

Ss. Cyril and Methodius University in Skopje

Faculty of Veterinary Medicine – Belgrade

Macedonian Veterinary Review

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 License.

Reduced glutathione (GSH) and homologous ram seminal plasma (HSP), used as additives in cryopreserving (CP) media prior to freezing, showed conflicting results...

Homologous Seminal Plasma and Glutathione Promote Pre-Capacitation Motility and Structural Stability of Cryopreserved Ram Spermatozoa

Published Online: Oct 15, 2019

Page range: 169 - 179

Received: Feb 01, 2019

Accepted: Aug 13, 2019

DOI: https://doi.org/10.2478/macvetrev-2019-0022

Keywords
ram, ejaculates, cryopreservation, homologous seminal plasma, reduced glutathione

© 2019 Martin Nikolovski et al., published by Sciendo

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 License.

Homologous Seminal Plasma and Glutathione Promote Pre-Capacitation Motility and Structural Stability of Cryopreserved Ram Spermatozoa

Published Online: Oct 15, 2019

Page range: 169 - 179

Received: Feb 01, 2019

Accepted: Aug 13, 2019

DOI: https://doi.org/10.2478/macvetrev-2019-0022

Keywordsram, ejaculates, cryopreservation, homologous seminal plasma, reduced glutathione

© 2019 Martin Nikolovski et al., published by Sciendo

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 License.

Keywords
ram, ejaculates, cryopreservation, homologous seminal plasma, reduced glutathione