<abstract xmlns="http://www.w3.org/1999/xhtml"><p>The anticoagulant rodenticides are the most commonly used toxicants to control rodents nowadays. Therefore, developing resistance to them is an issue of great importance for pest control. The aim of this study was to investigate the sensitivity of synanthropic rodents in the Stara Zagora region, Bulgaria to some of the most significant first (warfarin and coumatetralyl) and second (bromadiolone and brodifacoum) generation anticoagulants. Resistance tests were carried out by a standard protocol using lethal feeding period tests and blood clotting response tests according to the European and Mediterranean Plant Protection Organization (Paris, France) standard. Studies were performed on 278 wild synanthropic rodents – 67 house mice (<italic>Mus musculus</italic>), 153 roof rats <italic>(Rattus rattus)</italic> and 58 brown rats <italic>(Rattus norvegicus)</italic>. The rodents belonged to 11 populations inhabiting 9 animal farms in the region of Stara Zagora, Southern Bulgaria. High-level resistance to warfarinwas established in 100% of surveyed house mice and 92.1% of roof rats. Resistance to coumatetralyl was registered in 62.5% of the tested roof rats. Low-level resistance to bromadiolone was found in 38.5% of the surveyed roof rats and 23.1% of house mice. There was no resistance registered in brown rats. The sensitivity of all three rodent species to the strategic anticoagulant brodifacoum was high, and there were no signs of resistance. The results proved the resistance among synanthropic rodents and led to the conclusion that the resistance in house mice and roof rats to warfarin and coumatetralyl tends to be the main issue in pest control.</p></abstract>

The anticoagulant rodenticides are the most commonly used toxicants to control rodents nowadays. Therefore, developing resistance to them is an issue of great importance for pest control. The aim of this study was to investigate the sensitivity of synanthropic rodents in the Stara Zagora region, Bulgaria to some of the most significant first (warfarin and coumatetralyl) and second (bromadiolone and brodifacoum) generation anticoagulants. Resistance tests were carried out by a standard protocol using lethal feeding period tests and blood clotting response tests according to the European and Mediterranean Plant Protection Organization (Paris, France) standard. Studies were performed on 278 wild synanthropic rodents – 67 house mice (Mus musculus), 153 roof rats (Rattus rattus) and 58 brown rats (Rattus norvegicus). The rodents belonged to 11 populations inhabiting 9 animal farms in the region of Stara Zagora, Southern Bulgaria. High-level resistance to warfarinwas established in 100% of surveyed house mice and 92.1% of roof rats. Resistance to coumatetralyl was registered in 62.5% of the tested roof rats. Low-level resistance to bromadiolone was found in 38.5% of the surveyed roof rats and 23.1% of house mice. There was no resistance registered in brown rats. The sensitivity of all three rodent species to the strategic anticoagulant brodifacoum was high, and there were no signs of resistance. The results proved the resistance among synanthropic rodents and led to the conclusion that the resistance in house mice and roof rats to warfarin and coumatetralyl tends to be the main issue in pest control.

Anticoagulant Resistance in Synanthropic Rodents in the Stara Zagora Region, Bulgaria

Department of Epidemiology, Infectious Diseases and Preventive Medicine, Faculty of Veterinary Medicine

Macedonian Veterinary Review

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 License.

The anticoagulant rodenticides are the most commonly used toxicants to control rodents nowadays. Therefore, developing resistance to them is an issue of great...

Anticoagulant Resistance in Synanthropic Rodents in the Stara Zagora Region, Bulgaria

Published Online: Mar 28, 2019

Page range: 61 - 70

Received: Mar 02, 2018

Accepted: Nov 29, 2018

DOI: https://doi.org/10.2478/macvetrev-2019-0010

Keywords
resistance, anticoagulant rodenticides, synantropic rodents

© 2019 Georgi G. Zhelev et al., published by Sciendo

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 License.

Anticoagulant Resistance in Synanthropic Rodents in the Stara Zagora Region, Bulgaria

Published Online: Mar 28, 2019

Page range: 61 - 70

Received: Mar 02, 2018

Accepted: Nov 29, 2018

DOI: https://doi.org/10.2478/macvetrev-2019-0010

Keywordsresistance, anticoagulant rodenticides, synantropic rodents

© 2019 Georgi G. Zhelev et al., published by Sciendo

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 License.

Keywords
resistance, anticoagulant rodenticides, synantropic rodents