Genetic characterisation and local genotypes of canine parvovirus strains collected from pet dogs in central and eastern China during 2018–2019

Wen Hu; Liangyan Zheng; Xin Xu; Qiang Liu; Jun Ji; Lunguang Yao; Yunchao Kan; Yingzuo Bi

Open Access

Genetic characterisation and local genotypes of canine parvovirus strains collected from pet dogs in central and eastern China during 2018–2019

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| Nov 19, 2020

Journal of Veterinary Research

Volume 64 (2020): Issue 4 (December 2020)

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Cite

Page range: 477 - 486

Received: Jun 11, 2020

Accepted: Oct 26, 2020

DOI: https://doi.org/10.2478/jvetres-2020-0076

Keywords
amino acid mutation site, canine parvovirus, phylogenetic tree, VP2 identity

© 2020 W. Hu et al. published by Sciendo

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Genotypes and geographical distribution of the canine parvovirus (CPV) strains tested in this study

Evolutionary tree of canine parvovirus type-2 (CPV-2). Blue areas represent the 16 strains collected from Anhui Province, pink areas represent the 29 strains collected from Henan Province, and yellow areas represent the 16 strains collected from Zhejiang Province. Yellow stars denote the CPV-2a reference strain; purple stars denote the CPV-2b reference strain; and red stars denote the CPV-2c reference strain. Red strips indicate the CPV-2a strain; orange strips indicate the CPV-2b strain; and green strips indicate the CPV-2c strain

Antigenic epitope prediction and amino acid entropy rates of the VP2 capsid protein of canine parvovirus type 2. A: Antigen epitope prediction for CPV-VP2; B: Amino acid entropy rates for CPV-VP2

Reference strains used in the study

Strain	Accession number	Genotype	Place of isolation	Year of isolation
nn171025	MK332005	CPV-2a	Guangxi	2017
nn17101	MK332003	CPV-2a	Guangxi	2017
nn1693	MK332002	CPV-2b	Guangxi	2016
nn1681	MK331996	CPV-2b	Guangxi	2016
nn171105	MK332007	CPV-2c	Guangxi	2017
nn171024	MK332004	CPV-2c	Guangxi	2017
CPV-411b.us.9	EU659121	CPV-2b	The USA	1998
CPV-13.us.81	EU659118	CPV-2a	The USA	1981
CPV-6.us.80	EU659117	CPV-2	The USA	1980
Raccoon/ WI/ 37/ 10	JN867618	CPV-2a	The USA	2010
110/ 07-27	FJ005236	CPV-2c	The USA	2007
08-B	GU362934	CPV-2a	Italy	2008
260-00	MF177231	CPV-2a	Italy	2000
140/ 05	FJ005265	CPV-2b	Italy	2005
CPV /IZSSI /25835/ 09	KU508407	CPV-2c	Italy	2009
56/00	FJ222821	CPV-2c	Italy	2000
CPV/dog/HCM/20/2013	LC216910	CPV-2c	Indonesia	2013
Pome	EF599098	CPV-2c(a)	South Korea	2007
DH326	EF599097	CPV-2b	South Korea	2007
DH426	EF599096	CPV-2a	South Korea	2007
16M130	MH643886	CPV-2	South Korea	2016
2670/CPV-2c/2010/Ind	KX425920	CPV-2c	India	2010
CU267	MH711901	CPV-2c	Thailand	2017
TH011401	KT364589	CPV-2c	Thailand	2014
T37	CPU72698	CPV-2a	Taiwan	1996
T10	CPU72696	CPV-2b	Taiwan	1996
2017090801	MH127909	CPV-2c	Taiwan	2017
Protein (VP2)	KU244254	CPV-2c	Taiwan	2015
PV/PL/HeN02/08	EU441280	CPV-2a	Henan	2008
Henan42	KJ438805	CPV-2a	Henan	2013
CPV-HN1617	MF467229	CPV-2c	Henan	2016
CPV-zj18	KM386948	CPV-2b	Zhejiang	2014
CPV-zj7	KM386937	CPV-2a	Zhejiang	2014
Beijing	HQ883267	CPV-2a	Beijing	2010
BJ-1	MN101726	CPV-2a	Beijing	2018
2011-BJ-B43	KF803527	CPV-2b	Beijing	2011
2011-BJ-B6	KF803606	CPV-2b	Beijing	2011
CPV-SH1516	MG013488	CPV-2c	Shanghai	2017
Shanghai/04g/2016	KY937646	CPV-2a	Shanghai	2016
ShangHai/3-2/2016	KY937640	CPV-2a	Shanghai	2016
Shanghai/03g/2016	KY937637	CPV-2c	Shanghai	2016
CPVpf/2007(vaccine)	FJ197847	CPV-2	South Korea	2007
29/97(vaccine)	FJ222823	CPV-2b	N.I. no information	2008
CPV-GX1581	MF467242	CPV-2c	Guangxi	2015

Statistics of the main amino acid mutation sites in the VP2 capsid protein of canine parvovirus type 2 in Chinese and reference strains

Strains/GenBank Accession number	Mutation sites: amino acid residue

	5	30	130	370	426	440
EU659117-2/the USA/1980	A	G	V	Q	N	T
FJ197847-2/South Korea/2007/Vaccine	\	\	\	\	\	\
MH643886-2/South Korea/2016	\	\	\	\	\	\
EU659118-2a/the USA/1981	\	\	\	\	\	\
MF177231-2a/Italy/2000	\	\	\	\	\	\
GU362934-2a/Italy/2008	\	\	\	\	\	\
EU441280-2a/Henan/2008	\	\	\	\	\	\
HQ883267-2a/Beijing/2010	\	\	\	\	\	A
KJ438805-2a/Henan/2013	\	\	\	\	\	A
KY937646-2a/Shanghai/2016	\	\	\	\	\	A
MK332005-2a/Guangxi/2017	\	\	\	\	\	A
MN101726-2a/Beijing/2018	\	\	\	\	\	A
CPU72696-2b/Taiwan/1996	\	\	\	\	D	\
EU659121-2b/the USA/1998	\	\	\	\	D	\
FJ005265-2b/Italy/2005	\	\	\	\	D	\
KF803606-2b/Beijing/2011	\	\	\	\	D	\
MK332002-2b/Guangxi/2016	\	\	\	\	D	A
MK331996-2b/Guangxi/2016	\	\	\	\	D	A
KU508407-2c/Italy/2009	\	\	\	\	E	\
KX425920-2c/India/2010	\	\	\	\	E	\
KU244254-2c/Taiwan/2015	\	\	\	\	E	\
LC216910-2c/Indonesia/2013	G	\	\	R	E	\
MF467242-2c/Vaccine/2015	G	\	\	R	E	\
MF467229-2c/Henan/2016	G	\	\	R	E	\
MGo13488-2c/Shanghai/2017	G	\	\	R	E	\
CH-AH-D1	G	\	\	\	D	A
CH-AH-D2	\	\	\	R	E	\
CH-AH-D3	\	W	\	R	E	\
CH-AH-D4	G	\	\	R	E	\
CH-AH-D5	\	\	\	\	D	A
CH-AH-D6	G	\	\	R	E	\
CH-AH-D7	\	\	\	R	E	\
CH-AH-D8	\	\	\	R	E	\
CH-AH-D9	G	\	\	R	E	\
CH-AH-D10	G	\	\	R	E	\
CH-AH-D11	G	\	\	R	E	\
CH-AH-D12	G	\	\	R	E	\
CH-AH-D13	G	\	\	R	E	\
CH-AH-D14	\	\	\	\	\	A
CH-AH-D15	\	\	\	\	\	A
CH-AH-D16	\	\	\	\	D	A
CH-HN-D1	G	\	\	R	E	\
CH-HN-D2	G	\	\	R	E	\
CH-HN-D3	G	\	\	R	E	\
CH-HN-D4	G	\	\	R	E	\
CH-HN-D5	\	\	\	\	D	A
CH-HN-D6	G	\	\	R	E	\
CH-HN-D7	\	\	A	\	\	A
CH-HN-D8	\	\	\	R	E	\
CH-HN-D9	G	\	\	R	E	\
CH-HN-D10	G	\	\	R	E	\
CH-HN-D11	G	\	\	R	E	\
CH-HN-D12	G	\	\	R	E	\
CH-HN-D13	G	\	\	R	E	\
CH-HN-D14	\	\	\	\	\	A
CH-HN-D15	\	\	\	\	\	A
CH-HN-D16	\	\	\	\	D	A
CH-HN-D17	G	\	\	R	E	\
CH-HN-D18	\	\	\	\	D	A
CH-HN-D19	G	\	\	R	E	\
CH-HN-D20	\	\	\	\	D	A
CH-HN-D21	G	\	\	R	E	\
CH-HN-D22	G	\	\	R	E	\
CH-HN-D23	G	\	\	R	E	\
CH-HN-D24	G	\	\	R	E	\
CH-HN-D25	G	\	\	R	E	\
CH-HN-D26	G	\	\	R	E	\
CH-HN-D27	G	\	\	R	E	\
CH-HN-D28	G	\	\	R	E	\
CH-HN-D29	G	\	\	R	E	\
CH-ZJ-D1	G	\	\	R	E	\
CH-ZJ-D2	G	\	\	R	E	\
CH-ZJ-D3	G	\	\	R	E	\
CH-ZJ-D4	G	\	\	R	E	\
CH-ZJ-D5	G	\	\	R	E	\
CH-ZJ-D6	G	\	\	R	E	\
CH-ZJ-D7	G	\	\	R	E	\
CH-ZJ-D8	G	\	\	R	E	\
CH-ZJ-D9	G	\	\	R	E	\
CH-ZJ-D10	G	\	\	R	E	\
CH-ZJ-D11	G	\	\	R	E	\
CH-ZJ-D12	G	\	\	R	E	\
CH-ZJ-D13	G	\	\	R	E	\
CH-ZJ-D14	G	\	\	R	E	\
CH-ZJ-D15	G	\	\	R	E	\
CH-ZJ-D16	G	\	\	R	E	\

Clinical information on sources of Chinese canine parvovirus type 2 (CPV-2) obtained in this study

Strain	Genotype	Site	Date of sampling	Age	Breed	Sex	Vaccinated status	Accession number
CH-AH-D1	CPV-2b	Hefei	Oct. 7, 2018	5 months	Poodle	Female	Unvaccinated	MN119560
CH-AH-D2	CPV-2c	Anqing	Oct. 7, 2018	2 years	Poodle	Male	1 dose	MN119561
CH-AH-D3	CPV-2c	Suzhou	Oct. 8, 2018	1 year	Mixed	Female	2 doses	MN119562
CH-AH-D4	CPV-2c	Suzhou	Oct. 11, 2018	3 months	Poodle	Male	Unvaccinated	MN119563
CH-AH-D5	CPV-2b	Wuhu	Nov. 9, 2018	1 year	Mixed	Female	2 doses	MN119564
CH-AH-D6	CPV-2c	Anqing	Nov. 11, 2018	4 months	Poodle	Female	1 dose	MN119565
CH-AH-D7	CPV-2c	Suzhou	Nov. 13, 2018	45 days	Schnauzer	Male	Unvaccinated	MN119566
CH-AH-D8	CPV-2c	Hefei	Nov. 30, 2018	7 months	Mixed	Male	1 dose	MN119567
CH-AH-D9	CPV-2c	Wuhu	Dec. 1, 2018	6 months	Retriever	Male	Unvaccinated	MN119568
CH-AH-D10	CPV-2c	Suzhou	Dec. 3, 2018	3 months	Schnauzer	Female	Unvaccinated	MN119569
CH-AH-D11	CPV-2c	Anqing	Jan. 7, 2019	8 months	Mixed	Male	2 doses	MN119570
CH-AH-D12	CPV-2c	Suzhou	Feb. 7, 2019	4 months	Mixed	Female	Unvaccinated	MN119571
CH-AH-D13	CPV-2c	Wuhu	Mar. 11, 2019	2 months	Poodle	Female	1 dose	MN119572
CH-AH-D14	CPV-2a	Hefei	Mar. 16, 2019	52 days	Mixed	Female	Unvaccinated	MN119573
CH-AH-D15	CPV-2a	Anqing	Apr. 2, 2019	4 months	Retriever	Male	1 dose	MN119574
CH-AH-D16	CPV-2b	Suzhou	Apr. 3, 2019	3 months	Poodle	Female	Unvaccinated	MN119575
CH-HN-D1	CPV-2c	Zhengzhou	Oct. 3, 2018	40 days	Mixed	Male	Unvaccinated	MN119576
CH-HN-D2	CPV-2c	Hebi	Oct. 4, 2018	4 months	Mixed	Female	1 dose	MN119577
CH-HN-D3	CPV-2c	Nanyang	Oct. 8, 2018	10 months	Mixed	Male	1 dose	MN119578
CH-HN-D4	CPV-2c	Nanyang	Nov. 6, 2018	5 months	Retriever	Female	1 dose	MN119579
CH-HN-D5	CPV-2b	Luoyang	Nov. 7, 2018	4 months	Mixed	Male	Unvaccinated	MN119580
CH-HN-D6	CPV-2c	Xinxiang	Nov. 12, 2018	5 months	Schnauzer	Male	Unvaccinated	MN119581
CH-HN-D7	CPV-2a	Xinyang	Nov. 14, 2018	4 months	Mixed	Female	1 dose	MN119582
CH-HN-D8	CPV-2c	Luoyang	Nov. 16, 2018	2 months	Mixed	Female	1 dose	MN119583
CH-HN-D9	CPV-2c	Zhengzhou	Nov. 28, 2018	2 months	Schnauzer	Male	Unvaccinated	MN119584
CH-HN-D10	CPV-2c	Anyang	Nov. 29, 2018	4 months	Mixed	Female	1 dose	MN119585
CH-HN-D11	CPV-2c	Xinyang	Dec. 3, 2018	50 days	Mixed	Female	1 dose	MN119586
CH-HN-D12	CPV-2c	Nanyang	Jan. 1, 2019	3 months	Retriever	Male	Unvaccinated	MN119587
CH-HN-D13	CPV-2c	Shangqiu	Jan. 3, 2019	3 months	Mixed	Female	1 dose	MN119588
CH-HN-D14	CPV-2a	Anyang	Jan. 4, 2019	4 months	Mixed	Female	1 dose	MN119589
CH-HN-D15	CPV-2a	Zhengzhou	Jan. 5, 2019	1 month	Mixed	Male	Unvaccinated	MN119590
CH-HN-D16	CPV-2b	Anyang	Jan. 5, 2019	2 months	Poodle	Male	Unvaccinated	MN119591
CH-HN-D17	CPV-2c	Zhengzhou	Feb. 3, 2019	1 year	Mixed	Female	2 doses	MN119592
CH-HN-D18	CPV-2b	Nanyang	Feb. 4, 2019	4 months	Poodle	Male	Unvaccinated	MN119593
CH-HN-D19	CPV-2c	Xinxiang	Feb. 5, 2019	5 months	Mixed	Female	1 dose	MN119594
CH-HN-D20	CPV-2b	Hebi	Mar. 8, 2019	2 months	Poodle	Female	Unvaccinated	MN119595
CH-HN-D21	CPV-2c	Xinxiang	Mar. 9, 2019	5 months	Mixed	Male	1 dose	MN119596
CH-HN-D22	CPV-2c	Nanyang	Mar. 10, 2019	8 months	Retriever	Male	2 doses	MN119597
CH-HN-D23	CPV-2c	Hebi	Mar. 12, 2019	3 months	Mixed	Male	1 dose	MN119598
CH-HN-D24	CPV-2c	Luoyang	Mar. 15, 2019	2 months	Mixed	Female	1 dose	MN119599
CH-HN-D25	CPV-2c	Anyang	Mar. 29, 2019	2 months	Schnauzer	Female	Unvaccinated	MN119600
CH-HN-D26	CPV-2c	Zhengzhou	Mar. 31, 2019	4 months	Mixed	Male	Unvaccinated	MN119601
CH-HN-D27	CPV-2c	Luoyang	Apr. 1, 2019	1 year	Mixed	Female	1 dose	MN119602
CH-HN-D28	CPV-2c	Nanyang	Apr. 4, 2019	9 months	Mixed	Female	1 dose	MN119603
CH-HN-D29	CPV-2c	Zhengzhou	Apr. 5, 2019	4 months	Poodle	Male	Unvaccinated	MN119604
CH-ZJ-D1	CPV-2c	Huzhou	Oct. 2, 2018	1 month	Mixed	Female	1 dose	MN119605
CH-ZJ-D2	CPV-2c	Hangzhou	Oct. 3, 2018	3 months	Mixed	Male	Unvaccinated	MN119606
CH-ZJ-D3	CPV-2c	Jinhua	Oct. 4, 2018	2 months	Mixed	Female	1 dose	MN119607
CH-ZJ-D4	CPV-2c	Ningbo	Oct. 8, 2018	4 months	Retriever	Male	1 dose	MN119608
CH-ZJ-D5	CPV-2c	Hangzhou	Nov. 10, 2018	3 months	Mixed	Male	1 dose	MN119609
CH-ZJ-D6	CPV-2c	Ningbo	Nov. 11, 2018	6 months	Mixed	Female	2 doses	MN119610
CH-ZJ-D7	CPV-2c	Hangzhou	Nov. 17, 2018	2 months	Mixed	Female	Unvaccinated	MN119611
CH-ZJ-D8	CPV-2c	Jinhua	Dec. 1, 2018	5 months	Mixed	Male	1 dose	MN119612
CH-ZJ-D9	CPV-2c	Huzhou	Dec. 1, 2018	3 months	Mixed	Male	1 dose	MN119613
CH-ZJ-D10	CPV-2c	Shaoxing	Dec. 3, 2018	4 months	Retriever	Female	1 dose	MN119614
CH-ZJ-D11	CPV-2c	Jinhua	Jan. 4, 2019	7 months	Mixed	Male	2 doses	MN119615
CH-ZJ-D12	CPV-2c	Hangzhou	Feb. 6, 2019	2 months	Mixed	Male	1 dose	MN119616
CH-ZJ-D13	CPV-2c	Jinhua	Mar. 14, 2019	3 months	Mixed	Male	1 dose	MN119617
CH-ZJ-D14	CPV-2c	Shaoxing	Mar. 14, 2019	1 year	Mixed	Female	1 dose	MN119618
CH-ZJ-D15	CPV-2c	Hangzhou	Apr. 4, 2019	8 months	Mixed	Male	2 doses	MN119619
CH-ZJ-D16	CPV-2c	Jinhua	Apr. 4, 2019	9 months	Retriever	Female	2 doses	MN119620

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Genetic characterisation and local genotypes of canine parvovirus strains collected from pet dogs in central and eastern China during 2018–2019

Published Online: Nov 19, 2020

Page range: 477 - 486

Received: Jun 11, 2020

Accepted: Oct 26, 2020

DOI: https://doi.org/10.2478/jvetres-2020-0076

Keywords
amino acid mutation site, canine parvovirus, phylogenetic tree, VP2 identity

© 2020 W. Hu et al. published by Sciendo

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Fig. 1

Fig. 2

Fig. 3

Reference strains used in the study

Statistics of the main amino acid mutation sites in the VP2 capsid protein of canine parvovirus type 2 in Chinese and reference strains

Clinical information on sources of Chinese canine parvovirus type 2 (CPV-2) obtained in this study

Genetic characterisation and local genotypes of canine parvovirus strains collected from pet dogs in central and eastern China during 2018–2019

Published Online: Nov 19, 2020

Page range: 477 - 486

Received: Jun 11, 2020

Accepted: Oct 26, 2020

DOI: https://doi.org/10.2478/jvetres-2020-0076

Keywordsamino acid mutation site, canine parvovirus, phylogenetic tree, VP2 identity

© 2020 W. Hu et al. published by Sciendo

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Fig. 1

Fig. 2

Fig. 3

Reference strains used in the study

Statistics of the main amino acid mutation sites in the VP2 capsid protein of canine parvovirus type 2 in Chinese and reference strains

Clinical information on sources of Chinese canine parvovirus type 2 (CPV-2) obtained in this study

Keywords
amino acid mutation site, canine parvovirus, phylogenetic tree, VP2 identity