<abstract xmlns="http://www.w3.org/1999/xhtml">

<sec id="j_jvetres-2020-0059_s_005">
<h3>Introduction</h3>
<p>This study determined the presence of nitric oxide synthesis isoforms (nNOS, iNOS, and eNOS) in thoracic spinal cord segments and nodose ganglia of rats with gamma-irradiated livers.</p></sec>
<sec id="j_jvetres-2020-0059_s_006">
<h3>Material and Methods</h3>
<p>Male rats (n = 32) were divided into equal groups A, B, C, and D. In group A, the controls, no radiation was applied, while groups B, C, and D received 10 Gy of ionising gamma radiation. The rats of group B were euthanized at the end of the first day (d1), those of group C on the second day (d2), and those of group D on the third day (d3). The liver, spinal cord segments, and nodose ganglion tissues were dissected and fixed, and the liver sections were examined histopathologically. The other tissues were observed through a light microscope.</p></sec>
<sec id="j_jvetres-2020-0059_s_007">
<h3>Results</h3>
<p>Regeneration occurred at the end of d3 in hepatocytes which were radiation-damaged at the end of d1 and d2. On d1, some nNOS-positive staining was found in the neuronal cells of laminae I–III of the spinal cord and in neurons of the nodose ganglion, and on d3, some staining was observed in lamina X of the spinal cord, while none of note was in the nodose ganglion. Dense iNOS-positive staining was seen on d1 in the ependymal cells of the spinal cord and in the glial cells of the nodose ganglion, and on d3, there was still considerable iNOS staining in both tissues. There was clear eNOS-positive staining in the capillary endothelial cells of the spinal cord and light diffuse cytoplasmic staining in the neurons of the nodose ganglion on d1, and on d3, intense eNOS-positive staining was visible in several endothelial cells of the spinal cord, while light nuclear staining was recognised in the neurons of the nodose ganglion.</p></sec>
<sec id="j_jvetres-2020-0059_s_008">
<h3>Conclusion</h3>
<p>The nNOS, iNOS, and eNOS isoforms are activated in the spinal cord and nodose ganglion of rats after ionising radiation insult to the liver.</p></sec></abstract>

Introduction
This study determined the presence of nitric oxide synthesis isoforms (nNOS, iNOS, and eNOS) in thoracic spinal cord segments and nodose ganglia of rats with gamma-irradiated livers.

Material and Methods
Male rats (n = 32) were divided into equal groups A, B, C, and D. In group A, the controls, no radiation was applied, while groups B, C, and D received 10 Gy of ionising gamma radiation. The rats of group B were euthanized at the end of the first day (d1), those of group C on the second day (d2), and those of group D on the third day (d3). The liver, spinal cord segments, and nodose ganglion tissues were dissected and fixed, and the liver sections were examined histopathologically. The other tissues were observed through a light microscope.

Results
Regeneration occurred at the end of d3 in hepatocytes which were radiation-damaged at the end of d1 and d2. On d1, some nNOS-positive staining was found in the neuronal cells of laminae I–III of the spinal cord and in neurons of the nodose ganglion, and on d3, some staining was observed in lamina X of the spinal cord, while none of note was in the nodose ganglion. Dense iNOS-positive staining was seen on d1 in the ependymal cells of the spinal cord and in the glial cells of the nodose ganglion, and on d3, there was still considerable iNOS staining in both tissues. There was clear eNOS-positive staining in the capillary endothelial cells of the spinal cord and light diffuse cytoplasmic staining in the neurons of the nodose ganglion on d1, and on d3, intense eNOS-positive staining was visible in several endothelial cells of the spinal cord, while light nuclear staining was recognised in the neurons of the nodose ganglion.

Conclusion
The nNOS, iNOS, and eNOS isoforms are activated in the spinal cord and nodose ganglion of rats after ionising radiation insult to the liver.

An immunohistochemical study on the presence of nitric oxide synthase isoforms (nNOS, iNOS, eNOS) in the spinal cord and nodose ganglion of rats receiving ionising gamma radiation to their liver

Journal of Veterinary Research

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Introduction
This study determined the presence of nitric oxide synthesis isoforms (nNOS, iNOS, and eNOS) in thoracic spinal cord segments and nodose ganglia...

An immunohistochemical study on the presence of nitric oxide synthase isoforms (nNOS, iNOS, eNOS) in the spinal cord and nodose ganglion of rats receiving ionising gamma radiation to their liver

Published Online: Sep 16, 2020

Page range: 445 - 453

Received: Mar 06, 2020

Accepted: Aug 25, 2020

DOI: https://doi.org/10.2478/jvetres-2020-0059

Keywords
rats, ionising radiation, nitric oxide synthesis isoforms, nodose ganglion, spinal cord

© 2020 O. Yılmaz et al. published by Sciendo

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Fig. 1

Fig. 2

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An immunohistochemical study on the presence of nitric oxide synthase isoforms (nNOS, iNOS, eNOS) in the spinal cord and nodose ganglion of rats receiving ionising gamma radiation to their liver

Published Online: Sep 16, 2020

Page range: 445 - 453

Received: Mar 06, 2020

Accepted: Aug 25, 2020

DOI: https://doi.org/10.2478/jvetres-2020-0059

Keywordsrats, ionising radiation, nitric oxide synthesis isoforms, nodose ganglion, spinal cord

© 2020 O. Yılmaz et al. published by Sciendo

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Fig. 1

Fig. 2

Fig. 3

Fig. 4

Keywords
rats, ionising radiation, nitric oxide synthesis isoforms, nodose ganglion, spinal cord