Open Access

Molecular characterisation and genetic diversity of canine parvovirus type 2 prevalent in Central China

Wen Hu
Wen Hu
, 
Xin Xu
Xin Xu
, 
Qiang Liu
Qiang Liu
, 
Jun Ji
Jun Ji
, 
Yunchao Kan
Yunchao Kan
, 
Lunguang Yao
Lunguang Yao
, 
Yingzuo Bi
Yingzuo Bi
 and 
Qingmei Xie
Qingmei Xie
   | Sep 16, 2020
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Published Online: Sep 16, 2020
Page range: 347 - 354
Received: Feb 07, 2020
Accepted: Aug 13, 2020
DOI: https://doi.org/10.2478/jvetres-2020-0056
Keywords
© 2020 W. Hu et al. published by Sciendo
This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Fig. 1

Detection of CPV-VP2 strains by electrophoresis. M – 2000 Maker; 1-9 – CN/HN1601-1607, CN/HN1708, and CN/HN1709; N – negative control
Detection of CPV-VP2 strains by electrophoresis. M – 2000 Maker; 1-9 – CN/HN1601-1607, CN/HN1708, and CN/HN1709; N – negative control

Fig. 2

Geographical distribution and genotype of CPV isolates obtained in the study
Geographical distribution and genotype of CPV isolates obtained in the study

Fig. 3

Tertiary structural model of canine parvovirus (CPV) capsid protein (VP2) and the distribution of the main amino acid mutation residues. (a) – conserved structure of VP2 and (b) – structure of the mutant VP2 protein
Tertiary structural model of canine parvovirus (CPV) capsid protein (VP2) and the distribution of the main amino acid mutation residues. (a) – conserved structure of VP2 and (b) – structure of the mutant VP2 protein

Fig. 4

Phylogenetic tree based on VP2 gene sequences of canine parvovirus type 2 (CPV-2). Strains isolated from four central Chinese provinces in this study are marked with coloured triangles. The strains isolated from Hubei Province are indicated by black triangles; those from Anhui blue; those from Henan by orange; and those from Jiangsu green
Phylogenetic tree based on VP2 gene sequences of canine parvovirus type 2 (CPV-2). Strains isolated from four central Chinese provinces in this study are marked with coloured triangles. The strains isolated from Hubei Province are indicated by black triangles; those from Anhui blue; those from Henan by orange; and those from Jiangsu green

Information about the reference strains in our study

Strain Accession no. Genotype Place of isolation Submission date
nn171025 MK332005 CPV-2a Guangxi 2017
nn17101 MK332003 CPV-2a Guangxi 2017
nn1693 MK332002 CPV-2b Guangxi 2016
nn1681 MK331996 CPV-2b Guangxi 2016
nn171105 MK332007 CPV-2c Guangxi 2017
nn171024 MK332004 CPV-2c Guangxi 2017
CPV-411b.us.9 EU659121 CPV-2b the USA 1998
CPV-13.us.81 EU659118 CPV-2a the USA 1981
CPV-6.us.80 EU659117 CPV-2 the USA 1980
Raccoon/ WI/ 37/ 10 JN867618 CPV-2a the USA 2010
110/ 07-27 FJ005236 CPV-2c the USA 2007
08-B GU362934 CPV-2a Italy 2008
260-00 MF177231 CPV-2a Italy 2000
140/ 05 FJ005265 CPV-2b Italy 2005
CPV /IZSSI /25835/ 09 KU508407 CPV-2c Italy 2009
56/00 FJ222821 CPV-2c Italy 2000
CPV/dog/HCM/20/2013 LC216910 CPV-2c Indonesia 2013
Pome EF599098 CPV2c(a) South Korea 2007
DH326 EF599097 CPV-2b South Korea 2007
DH426 EF599096 CPV-2a South Korea 2007
16M130 MH643886 CPV-2 South Korea 2016
2670/CPV-2c/2010/Ind KX425920 CPV-2c India 2010
CU267 MH711901 CPV-2c Thailand 2017
TH011401 KT364589 CPV-2c Thailand 2014
T37 CPU72698 CPV-2a Taiwan 1996
T10 CPU72696 CPV-2b Taiwan 1996
2017090801 MH127909 CPV-2c Taiwan 2017
Protein(VP2) KU244254 CPV-2c Taiwan 2015
PV/PL/HeN02/08 EU441280 CPV-2a Henan 2008
Henan42 KJ438805 CPV-2a Henan 2013
CPV-HN1617 MF467229 CPV-2c Henan 2016
CPV-zj18 KM386948 CPV-2b Zhejiang 2014
CPV-zj7 KM386937 CPV-2a Zhejiang 2014
Beijing HQ883267 CPV-2a Beijing 2010
BJ-1 MN101726 CPV-2a Beijing 2018
2011-BJ-B43 KF803527 CPV-2b Beijing 2011
2011-BJ-B6 KF803606 CPV-2b Beijing 2011
CPV-SH1516 MG013488 CPV-2c Shanghai 2017
Shanghai/04g/2016 KY937646 CPV-2a Shanghai 2016
ShangHai/3-2/2016 KY937640 CPV2a Shanghai 2016
Shanghai/03g/2016 KY937637 CPV-2c Shanghai 2016
CPVpf/2007(vaccine) FJ197847 CPV-2 South Korea 2007
29/97(vaccine) FJ222823 CPV-2b N.I.

no information

 2008
CPV-GX1581 MF467242 CPV-2c Guangxi 2015
CPV-HN1506 MF467240 New-2a Henan 2017
RVC57 KNA 2016 KY399053 New-2a Saint Kitts and Nevis 2016
SY40 KY625992 New-2a China 2016
PU4 KC429669 New-2a India 2011
BJ15-15 KT162046 New-2a Beijing 2015
SY38 KY625998 New-2b China 2016
BJ15-11 KT162026 New-2b Beijing 2015
VP2 KR058183 New-2b China 2013
CPV-HN1506 MF467239 New-2b Henan 2015

Epidemiological data of the 56 strains in the study and the reference strains for genotyping

Strains Accession nos. Origin Vaccination record Age CPV type Substitution of amino acid residues in VP2
5 267 297 324 370 426 440
CPV-6.us.80 EU659117 the USA, 1980 N.I.

no information

 N.I. CPV-2 A F S Y Q N T
CPV-13.us.81 EU659118 the USA, 1981 N.I. N.I. CPV-2a A F S Y Q N A
CPV-411b.us.98 EU659121 the USA, 1998 N.I. N.I. CPV-2b A F A Y Q N A
CPV-HN1506 MF467240 Henan, 2017 N.I. N.I. New-2a A Y A I Q N A
RVC57 KNA KY399053 SNA, 2016 N.I. N.I. New-2a A F A I Q N A
BJ15-15 KT162046 Beijing, 2015 N.I. N.I. New-2a A Y A I Q N A
BJ15-11 KT162026 Beijing, 2015 N.I. N.I. New-2b A Y A I Q D A
VP2 KR058183 China, 2013 N.I. N.I. New-2b A Y A I Q D A
CPV-HN1506 MF467239 Henan, 2015 N.I. N.I. New-2b A Y A I Q D A
CPV dog HCM LC216910 Indonesia,2013 N.I. N.I. CPV-2c G Y A I R E T
CPV-HN1617 MF467229 Henan, 2016 N.I. N.I. CPV-2c G Y A I R E T
CPV-SH1516 MG013488 Shanghai, 2017 N.I. N.I. CPV-2c G Y A I R E T
CN/HN1601 MK517966 Henan, 2016 N.I. 3 m CPV-2c A Y A I R E T
CN/HN1602 MK517967 Henan, 2016 1 dose 2 m CPV-2c A Y A I R E T
CN/HN1603 MK517968 Henan, 2016 2 doses 5 m CPV-2c G Y A I R E T
CN/HN1604 MK517969 Henan, 2016 1 dose 2 m CPV-2c A Y A I R E T
CN/HN1605 MK517970 Henan, 2016 1 dose 2 m CPV-2c G Y A I R E T
CN/HN1606 MK517971 Henan, 2016 2 doses 7 m New-2a A Y A I Q N A
CN/HN1607 MK517972 Henan, 2016 1 dose 2 m CPV-2c G Y A I R E T
CN/HN1708 MK517973 Henan, 2017 N.I. 3 m CPV-2c A Y A I R E T
CN/HN1709 MK517974 Henan, 2017 N.I. 4 m New-2a A Y A I Q N A
CN/HN1710 MK517975 Henan, 2017 1 dose 3 m New -2a A Y A I Q N A
CN/HN1711 MK517976 Henan, 2017 1 dose 2 m CPV-2c A Y A I R E T
CN/HN1712 MK517977 Henan, 2017 N.I. 5 m New-2a A Y A I Q N A
CN/HN1713 MK517978 Henan, 2017 1 dose 42 d CPV-2c G Y A L R E T
CN/HN1714 MK517979 Henan, 2017 1 dose 2 m CPV-2c A Y A Y R E T
CN/HN1715 MK517980 Henan, 2017 1 dose 5 m New-2a A Y A I Q N A
CN/HN1716 MK517981 Henan, 2017 2 doses 4 m CPV-2c G Y A I R E T
CN/HN1717 MK517982 Henan, 2017 N.I. 2 m New-2a G Y A I Q N A
CN/HN1718 MK517983 Henan, 2017 1 dose 37 d New-2b A Y A I Q D A
CN/HN1719 MK517984 Henan, 2017 1 dose 2 m New-2b A Y A I Q D A
CN/HN1720 MK517985 Henan, 2017 2 doses 4 m New-2a G Y A I Q N A
CN/HN1721 MK517986 Henan, 2017 1 dose 2 m CPV-2c G Y A I R E T
CN/HN1722 MK517987 Henan, 2017 N.I. 6 m CPV-2c A Y A I R E T
CN/HN1723 MK517988 Henan, 2017 1 dose 5 m CPV-2c A Y A I R E T
CN/HB1601 MK517989 Hubei, 2016 N.I. 40 d CPV-2c G Y A I R E T
CN/HB1602 MK517990 Hubei, 2016 1 dose 5 m CPV-2c G Y A I R E T
CN/HB1603 MK517991 Hubei, 2016 1 dose 3 m CPV-2c G Y A I R E T
CN/HB1704 MK517992 Hubei, 2017 1 dose 3 m CPV-2c G Y A I R E A
CN/HB1705 MK517993 Hubei, 2017 2 doses 4 m CPV-2c A Y A I R E T
CN/HB1706 MK517994 Hubei, 2017 1 dose 2 m CPV-2c A Y A I R E T
CN/HB1707 MK517995 Hubei, 2017 N.I. 3 m New-2a A Y A I Q N A
CN/HB1708 MK517996 Hubei, 2017 1 dose 33 d CPV-2c A Y A I R E T
CN/HB1709 MK517997 Hubei, 2017 1 dose 2 m CPV-2c G Y A I R E T
CN/HB1710 MK517998 Hubei, 2017 2 doses 4 m CPV-2c G Y A I R E T
CN/HB1711 MK517999 Hubei, 2017 N.I. 6 m CPV-2c A Y A I R E T
CN/HB1712 MK518000 Hubei, 2017 1 dose 39 d New-2a A Y A I Q N A
CN/HB1713 MK518001 Hubei, 2017 1 dose 3 m CPV-2c G Y A L R E T
CN/HB1714 MK518002 Hubei, 2017 N.I. 5 m CPV-2a A Y S Y Q N T
CN/HB1715 MK518003 Hubei, 2017 1 dose 2 m New-2a G Y A I R E T
CN/AH1601 MK518004 Anhui, 2016 2 doses 6 m CPV-2c G Y A I R E T
CN/AH1602 MK518005 Anhui, 2016 N.I. 4 m CPV-2c G Y A I R E T
CN/AH1603 MK518006 Anhui, 2016 1 dose 3 m CPV-2c G Y A I R E T
CN/AH1604 MK518007 Anhui, 2016 1 dose 5 m New-2b A Y A I R D T
CN/AH1705 MK518008 Anhui, 2017 N.I. 8 m CPV-2c G Y A I R E T
CN/AH1706 MK518009 Anhui, 2017 1 dose 3 m New-2b A Y A I Q D A
CN/AH1707 MK518010 Anhui, 2017 N.I. 2 m CPV-2c A Y A I R E T
CN/AH1708 MK518011 Anhui, 2017 N.I. 3 m New-2b G Y A I Q D T
CN/AH1709 MK518012 Anhui, 2017 2 doses 4 m CPV-2c A Y A I R E T
CN/AH1710 MK518013 Anhui, 2017 1 dose 5 m New -2a G Y A I Q N T
CN/JS1601 MK518014 Jiangsu, 2016 N.I. 3 m CPV-2c A Y A I R E T
CN/JS1602 MK518015 Jiangsu, 2016 1 dose 2 m CPV-2c G Y A I R E T
CN/JS1603 MK518016 Jiangsu, 2016 N.I. 41 d New-2a A Y A I Q N A
CN/JS1704 MK518017 Jiangsu, 2017 N.I. 4 m New-2a G Y A I Q N A
CN/JS1705 MK518018 Jiangsu, 2017 1 dose 4 m CPV-2c A Y A I R E T
CN/JS1706 MK518019 Jiangsu, 2017 N.I. 2 m CPV-2c A Y A I R E T
CN/JS1707 MK518020 Jiangsu, 2017 2 doses 5 m CPV-2c G Y A I R E T
CN/JS1708 MK518021 Jiangsu, 2017 1 dose 3 m CPV-2c A Y A I R E T
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