<abstract xmlns="http://www.w3.org/1999/xhtml"><sec id="j_jvetres-2020-0043_s_005_w2aab3b7c83b1b6b1aab1c14b1Aa"><h3>Introduction</h3><p>Although peripheral blood analysis has become increasingly automated, microscopy is the only available method for the diagnosis of anisocytosis and poikilocytosis. The aims of the study were to compare RBC volume data obtained with two different analysers and by manual assessment of smears and to compare this data between dogs in various stages of heart failure secondary to degenerative mitral valvular (DMV) disease. The impact of diuretic administration on RBC morphology was also assessed.</p></sec><sec id="j_jvetres-2020-0043_s_006_w2aab3b7c83b1b6b1aab1c14b2Aa"><h3>Material and Methods</h3><p>Sixty-eight dogs, 56 in different stages of DMV disease and 12 as healthy controls, were studied. Impedance and flow cytometry haematological analyses were performed for each animal. Additionally, two smears were prepared for manual analysis. RBC structure, staining, and size differences were recorded.</p></sec><sec id="j_jvetres-2020-0043_s_007_w2aab3b7c83b1b6b1aab1c14b3Aa"><h3>Results</h3><p>There were no significant differences between the blood morphological parameters assessed using haematological analysers nor between dogs receiving diuretic treatment and those not treated. Based on the manual smear, significantly higher erythrocyte anisocytosis was observed in the dogs with symptomatic DMV disease than in the control group.</p></sec><sec id="j_jvetres-2020-0043_s_008_w2aab3b7c83b1b6b1aab1c14b4Aa"><h3>Conclusion</h3><p>Haematological analysers based on impedance and flow cytometry provide reliable and comparable morphological results in dogs with heart failure. However, microscopic assessment of blood smears is a more reliable tool to detect erythrocyte anisocytosis.</p></sec></abstract>

IntroductionAlthough peripheral blood analysis has become increasingly automated, microscopy is the only available method for the diagnosis of anisocytosis and poikilocytosis. The aims of the study were to compare RBC volume data obtained with two different analysers and by manual assessment of smears and to compare this data between dogs in various stages of heart failure secondary to degenerative mitral valvular (DMV) disease. The impact of diuretic administration on RBC morphology was also assessed.Material and MethodsSixty-eight dogs, 56 in different stages of DMV disease and 12 as healthy controls, were studied. Impedance and flow cytometry haematological analyses were performed for each animal. Additionally, two smears were prepared for manual analysis. RBC structure, staining, and size differences were recorded.ResultsThere were no significant differences between the blood morphological parameters assessed using haematological analysers nor between dogs receiving diuretic treatment and those not treated. Based on the manual smear, significantly higher erythrocyte anisocytosis was observed in the dogs with symptomatic DMV disease than in the control group.ConclusionHaematological analysers based on impedance and flow cytometry provide reliable and comparable morphological results in dogs with heart failure. However, microscopic assessment of blood smears is a more reliable tool to detect erythrocyte anisocytosis.

Analysis of red blood cell parameters in dogs with various stages of degenerative mitral valve disease

Journal of Veterinary Research

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

IntroductionAlthough peripheral blood analysis has become increasingly automated, microscopy is the only available method for the diagnosis of anisocytosis and...

Analysis of red blood cell parameters in dogs with various stages of degenerative mitral valve disease

Published Online: Jun 16, 2020

Page range: 325 - 332

Received: Oct 28, 2019

Accepted: Jun 02, 2020

DOI: https://doi.org/10.2478/jvetres-2020-0043

Keywords
dog, anisocytosis, mitral valve disease, haematology

© 2020 E. Kumiega et al. published by Sciendo

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Analysis of red blood cell parameters in dogs with various stages of degenerative mitral valve disease

Published Online: Jun 16, 2020

Page range: 325 - 332

Received: Oct 28, 2019

Accepted: Jun 02, 2020

DOI: https://doi.org/10.2478/jvetres-2020-0043

Keywordsdog, anisocytosis, mitral valve disease, haematology

© 2020 E. Kumiega et al. published by Sciendo

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Keywords
dog, anisocytosis, mitral valve disease, haematology