<abstract xmlns="http://www.w3.org/1999/xhtml">

<sec id="j_jvetres-2020-0024_s_005">
<h3>Introduction</h3>
<p>Porcine epidemic diarrhoea virus (PEDV) infection causes watery diarrhoea, vomiting, anorexia, and weight loss, especially among neonatal piglets, inflicting on them morbidity and mortality potentially reaching 90%–100%. Despite it being known that certain mammalian cell phases are arrested by PEDV, the mechanisms have not been elucidated, and PEDV pathogenesis is poorly understood. This study determined the effect of an epidemic PEDV strain on cell cycle progression.</p>
</sec>
<sec id="j_jvetres-2020-0024_s_006">
<h3>Material and Methods</h3>
<p>We observed the effect of the PEDV SHpd/2012 strain on an infected Vero cell cycle through flow cytometry and Western blot, investigating the interrelationships of cell-cycle arrest, the DNA damage–signalling pathway caused by PEDV and the phosphorylation levels of the key molecules Chk.2 and H2A.X involved upstream and downstream in this pathway.</p>
</sec>
<sec id="j_jvetres-2020-0024_s_007">
<h3>Results</h3>
<p>PEDV induced Vero cell-cycle arrest at the G1/G0 phase. The phosphorylation levels of Chk.2 and H2A.X increased with the prolongation of PEDV infection, and no significant cell-cycle arrest was observed after treatment with ATM or Chk.2 inhibitors. The proliferation of PEDV was also inhibited by treatment with ATM or Chk.2 inhibitors.</p>
</sec>
<sec id="j_jvetres-2020-0024_s_008">
<h3>Conclusion</h3>
<p>PEDV-induced cell-cycle arrest is associated with activation of DNA damage–signalling pathways. Our findings elucidate the molecular basis of PEDV replication and provide evidence to support further evaluation of PEDV pathogenesis.</p>
</sec>

</abstract>

Introduction
Porcine epidemic diarrhoea virus (PEDV) infection causes watery diarrhoea, vomiting, anorexia, and weight loss, especially among neonatal piglets, inflicting on them morbidity and mortality potentially reaching 90%–100%. Despite it being known that certain mammalian cell phases are arrested by PEDV, the mechanisms have not been elucidated, and PEDV pathogenesis is poorly understood. This study determined the effect of an epidemic PEDV strain on cell cycle progression.


Material and Methods
We observed the effect of the PEDV SHpd/2012 strain on an infected Vero cell cycle through flow cytometry and Western blot, investigating the interrelationships of cell-cycle arrest, the DNA damage–signalling pathway caused by PEDV and the phosphorylation levels of the key molecules Chk.2 and H2A.X involved upstream and downstream in this pathway.


Results
PEDV induced Vero cell-cycle arrest at the G1/G0 phase. The phosphorylation levels of Chk.2 and H2A.X increased with the prolongation of PEDV infection, and no significant cell-cycle arrest was observed after treatment with ATM or Chk.2 inhibitors. The proliferation of PEDV was also inhibited by treatment with ATM or Chk.2 inhibitors.


Conclusion
PEDV-induced cell-cycle arrest is associated with activation of DNA damage–signalling pathways. Our findings elucidate the molecular basis of PEDV replication and provide evidence to support further evaluation of PEDV pathogenesis.

Porcine epidemic diarrhoea virus induces cell-cycle arrest through the DNA damage-signalling pathway

Journal of Veterinary Research

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Introduction
Porcine epidemic diarrhoea virus (PEDV) infection causes watery diarrhoea, vomiting, anorexia, and weight loss, especially among neonatal piglets,...

Porcine epidemic diarrhoea virus induces cell-cycle arrest through the DNA damage-signalling pathway

Published Online: Mar 24, 2020

Page range: 25 - 32

Received: Jun 07, 2019

Accepted: Mar 09, 2020

DOI: https://doi.org/10.2478/jvetres-2020-0024

Keywords
porcine epidemic diarrhoea virus, cell cycle, virus proliferation, DNA damage, signalling pathways

© 2020 Y.R. Luo et al. published by Sciendo

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Porcine epidemic diarrhoea virus induces cell-cycle arrest through the DNA damage-signalling pathway

Published Online: Mar 24, 2020

Page range: 25 - 32

Received: Jun 07, 2019

Accepted: Mar 09, 2020

DOI: https://doi.org/10.2478/jvetres-2020-0024

Keywordsporcine epidemic diarrhoea virus, cell cycle, virus proliferation, DNA damage, signalling pathways

© 2020 Y.R. Luo et al. published by Sciendo

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Keywords
porcine epidemic diarrhoea virus, cell cycle, virus proliferation, DNA damage, signalling pathways