<abstract xmlns="http://www.w3.org/1999/xhtml">

<sec id="j_jvetres-2020-0021_s_005">
<h3>Introduction</h3>
<p>The aim of this study was to present two outbreaks of bovine abortion due to <italic>Leptospira</italic> infection in cattle herds located in the northern part of Sicily (Italy). The animals were positive for <italic>Leptospira interrogans</italic> serogroup Sejroe serovar Hardjo in a microscopic agglutination test (MAT).</p></sec>
<sec id="j_jvetres-2020-0021_s_006">
<h3>Material and Methods</h3>
<p>A total of 23 Charolaise cows (farm A) and 75 Limousine bulls and Cinisara and Modicana cows (farm B) were enrolled in this study. The blood samples were collected from all subjects at the following time points: before a cycle of intramuscular treatment with oxytetracycline dihydrate (T0), after 5–6 weeks from the treatment (T1), and every 10 weeks until seronegativisation (T2 in Farm A and T3 in Farm B). A serological test (MAT) was used for the diagnosis of leptospirosis.</p></sec>
<sec id="j_jvetres-2020-0021_s_007">
<h3>Results</h3>
<p>Two samples from farm A (2/23) and 29 samples from farm B (29/75) were positive to <italic>Leptospira interrogans</italic>, serogroup Sejroe, serovar Hardjo in the MAT. <italic>Leptospira</italic> spp. DNA was detected by real-time PCR in the urine sample of one positive cow on farm A, and in placenta and brain samples belonging to one aborted foetus on farm B.</p></sec>
<sec id="j_jvetres-2020-0021_s_008">
<h3>Conclusion</h3>
<p>It is important to use serological and molecular diagnostic techniques complementarily to identify infected individuals.</p></sec></abstract>

Introduction
The aim of this study was to present two outbreaks of bovine abortion due to Leptospira infection in cattle herds located in the northern part of Sicily (Italy). The animals were positive for Leptospira interrogans serogroup Sejroe serovar Hardjo in a microscopic agglutination test (MAT).

Material and Methods
A total of 23 Charolaise cows (farm A) and 75 Limousine bulls and Cinisara and Modicana cows (farm B) were enrolled in this study. The blood samples were collected from all subjects at the following time points: before a cycle of intramuscular treatment with oxytetracycline dihydrate (T0), after 5–6 weeks from the treatment (T1), and every 10 weeks until seronegativisation (T2 in Farm A and T3 in Farm B). A serological test (MAT) was used for the diagnosis of leptospirosis.

Results
Two samples from farm A (2/23) and 29 samples from farm B (29/75) were positive to Leptospira interrogans, serogroup Sejroe, serovar Hardjo in the MAT. Leptospira spp. DNA was detected by real-time PCR in the urine sample of one positive cow on farm A, and in placenta and brain samples belonging to one aborted foetus on farm B.

Conclusion
It is important to use serological and molecular diagnostic techniques complementarily to identify infected individuals.

<italic>Leptospira interrogans</italic> serogroup Sejroe serovar Hardjo in aborting cows: two herd cases in Sicily (Italy)

Journal of Veterinary Research

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

 serogroup Sejroe serovar Hardjo in aborting cows: two herd cases in Sicily (Italy)

Introduction
The aim of this study was to present two outbreaks of bovine abortion due to Leptospira infection in cattle herds located in the northern part of...

Leptospira interrogans serogroup Sejroe serovar Hardjo in aborting cows: two herd cases in Sicily (Italy)

Published Online: Mar 24, 2020

Page range: 73 - 78

Received: Jul 04, 2019

Accepted: Mar 02, 2020

DOI: https://doi.org/10.2478/jvetres-2020-0021

Keywords
cattle, leptospirosis, microscopic agglutination test, PCR, zoonosis

© 2020 F. Grippi et al. published by Sciendo

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Leptospira interrogans serogroup Sejroe serovar Hardjo in aborting cows: two herd cases in Sicily (Italy)

Published Online: Mar 24, 2020

Page range: 73 - 78

Received: Jul 04, 2019

Accepted: Mar 02, 2020

DOI: https://doi.org/10.2478/jvetres-2020-0021

Keywordscattle, leptospirosis, microscopic agglutination test, PCR, zoonosis

© 2020 F. Grippi et al. published by Sciendo

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Keywords
cattle, leptospirosis, microscopic agglutination test, PCR, zoonosis