<abstract xmlns="http://www.w3.org/1999/xhtml">

<sec id="j_jvetres-2020-0003_s_005">
<h3>Introduction</h3>
<p>Since 2009, Poland has been recognised as a country officially free of bovine tuberculosis (bTB), although in each year of the last five there were from 8 to 18 outbreaks of the disease. In 2008–2016, the largest number of cattle infected with bovine mycobacteria were eliminated in the Masovian Province (the central region of Poland) and the largest number of outbreaks of this zoonosis were recorded in this area. The close proximity of farms where bTB was found led to the suspicion that tuberculosis could have been transmitted between the affected herds. The aim of the study was the molecular characterisation of the pertinent <italic>M. bovis</italic>/<italic>caprae</italic> strains and determination of the epidemiological relationship of various bTB outbreaks.</p></sec>
<sec id="j_jvetres-2020-0003_s_006">
<h3>Material and Methods</h3>
<p>The material for microbiological tests came from 119 cattle (<italic>Bos taurus</italic>) from nine herds located in five provinces, neighbouring the Masovian Province.</p></sec>
<sec id="j_jvetres-2020-0003_s_007">
<h3>Results</h3>
<p>Laboratory tests of tissue material gave results confirming tuberculosis in 54 (45%) animals. All strains belonged to the <italic>Mycobacterium bovis</italic> species. A two-step analysis of genetic affinity allowed 50 strains to be identified as phylogenetically closely related and separated between three genetic clusters consisting of 2 to 27 strains.</p></sec>
<sec id="j_jvetres-2020-0003_s_008">
<h3>Conclusion</h3>
<p>Based on the results of genotyping, bTB outbreaks were found in three herds, and three transmission chains were identified among these herds.</p></sec>
</abstract>

Introduction
Since 2009, Poland has been recognised as a country officially free of bovine tuberculosis (bTB), although in each year of the last five there were from 8 to 18 outbreaks of the disease. In 2008–2016, the largest number of cattle infected with bovine mycobacteria were eliminated in the Masovian Province (the central region of Poland) and the largest number of outbreaks of this zoonosis were recorded in this area. The close proximity of farms where bTB was found led to the suspicion that tuberculosis could have been transmitted between the affected herds. The aim of the study was the molecular characterisation of the pertinent M. bovis/caprae strains and determination of the epidemiological relationship of various bTB outbreaks.

Material and Methods
The material for microbiological tests came from 119 cattle (Bos taurus) from nine herds located in five provinces, neighbouring the Masovian Province.

Results
Laboratory tests of tissue material gave results confirming tuberculosis in 54 (45%) animals. All strains belonged to the Mycobacterium bovis species. A two-step analysis of genetic affinity allowed 50 strains to be identified as phylogenetically closely related and separated between three genetic clusters consisting of 2 to 27 strains.

Conclusion
Based on the results of genotyping, bTB outbreaks were found in three herds, and three transmission chains were identified among these herds.

Molecular characterisation of the <italic>Mycobacterium bovis</italic> causing bovine tuberculosis outbreaks in Poland

Journal of Veterinary Research

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Molecular characterisation of the  causing bovine tuberculosis outbreaks in Poland

Introduction
Since 2009, Poland has been recognised as a country officially free of bovine tuberculosis (bTB), although in each year of the last five there...

No	Spoligotype	MIRU-VNTR type	LOCUS

			4	10	16	26	31	40	424	577	2165	2401	3690	4156	2163	1955	4052
1	BOVIS 595	424722356422327	4	2	4	7	2	2	3	5	6	4	2	2	3	2	7
2	BOVIS 595	424722357422325	4	2	4	7	2	2	3	5	7	4	2	2	3	2	5
3	BOVIS 595	425532355422434	4	2	5	5	3	2	3	5	5	4	2	2	4	3	4
4	BOVIS 595	424721374422525	4	2	4	7	2	1	3	7	4	4	2	2	5	2	5
5	BOVIS 595	424732357422325	4	2	4	7	3	2	3	5	7	4	2	2	3	2	5
6	BOVIS 595	424731357432215	4	2	4	7	3	1	3	5	7	4	3	2	2	1	5
7	BOVIS 595	424832354422437	4	2	4	8	3	2	3	5	4	4	2	2	4	3	7
8	BOVIS 595	424731374452525	4	2	4	7	3	1	3	7	4	4	5	2	5	2	5
9	BOVIS 595	424731374422525	4	2	4	7	3	1	3	7	4	4	2	2	5	2	5

No	Spoligotype	MIRU-VNTR (number of strains)	Number of strains in the cluster	Number of herds with strains in the cluster
I	BOVIS 595¹ SB2220²	425532355422434 (21)	21	2

II	BOVIS 595¹ SB2220²	424722357422325 (18) 424732357422325 (9)	27	2

III	Not registered	424731374452525 (1) 424731374422525 (1)	2	2

Molecular characterisation of the Mycobacterium bovis causing bovine tuberculosis outbreaks in Poland

Published Online: Jan 20, 2020

Page range: 45 - 50

Received: May 23, 2019

Accepted: Jan 13, 2020

DOI: https://doi.org/10.2478/jvetres-2020-0003

Keywords
cattle, bovine tuberculosis, Poland

© 2020 M. Krajewska-Wędzina et al. published by Sciendo

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Fig. 1

Nine MIRU-VNTR patterns identified among 54 strains

Molecular clusters identified among 50 strains by spoligotypes and MIRU-VNTR patterns

Molecular characterisation of the Mycobacterium bovis causing bovine tuberculosis outbreaks in Poland

Published Online: Jan 20, 2020

Page range: 45 - 50

Received: May 23, 2019

Accepted: Jan 13, 2020

DOI: https://doi.org/10.2478/jvetres-2020-0003

Keywordscattle, bovine tuberculosis, Poland

© 2020 M. Krajewska-Wędzina et al. published by Sciendo

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Fig. 1

Nine MIRU-VNTR patterns identified among 54 strains

Molecular clusters identified among 50 strains by spoligotypes and MIRU-VNTR patterns

Keywords
cattle, bovine tuberculosis, Poland